| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,721,680 | G→T | 13.4% | intergenic (‑39/+25) | PP_4178 ← / ← htpG | dienelactone hydrolase family protein/chaperone protein HtpG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,721,680 | 0 | G | T | 13.4% | 167.4 / 10.8 | 60 | intergenic (‑39/+25) | PP_4178/htpG | dienelactone hydrolase family protein/chaperone protein HtpG |
| Reads supporting (aligned to +/- strand): ref base G (24/27); new base T (3/5); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CCATCACCAGCCCTGGGCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGATGTGCGACAGCTCGGC > NC_002947/4721533‑4721821 | ccATCACCAGCCCTGGGCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGaa < 1:1441998/150‑1 (MQ=255) gtgcgcTGGGCCGCCCAGGGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCt > 1:316803/6‑150 (MQ=255) aGCCCTGGGCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCtt > 1:1412153/1‑150 (MQ=255) ccTGGGCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGc > 1:1318169/1‑150 (MQ=255) cTGGGCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCt < 2:1318169/150‑1 (MQ=255) ggggCGGCTCAGCGCCCCTGGCTCATAGACCAAACCGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGCCGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAACGCCCGCCGAAGCGGGCTTTGCtg < 1:435205/149‑1 (MQ=255) gggCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCtgt < 2:1451633/150‑1 (MQ=255) gggCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCtgt < 2:1521990/150‑1 (MQ=255) tgttcgatcaggcggccctggcggccggtgacagcctgaaggacccggcggcttacgttcgtcgcctgaacaagctactggtcgagctgtctgcttgagttgatgcacagaAAAGCCCGCCGAAGCGGGCTTTg < 1:1229815‑M1/23‑1 (MQ=255) aCCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGc > 2:40577/1‑150 (MQ=255) cccGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCa < 1:1320916/150‑1 (MQ=255) gCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACa < 2:1388892/150‑1 (MQ=255) tCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGc > 2:607749/1‑150 (MQ=255) cATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCt < 1:373946/150‑1 (MQ=255) aTAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTg > 1:261203/1‑123 (MQ=255) aTAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTg < 2:261203/123‑1 (MQ=255) aaCGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCaa > 1:737441/1‑131 (MQ=255) aaCGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCaa < 2:737441/131‑1 (MQ=255) tttCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGt > 2:568339/1‑150 (MQ=255) gTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGcga > 1:961486/1‑150 (MQ=255) tCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGcgac > 2:1523293/1‑150 (MQ=255) gCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAAc < 1:607749/150‑1 (MQ=255) gCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAAc < 1:1054153/150‑1 (MQ=255) cAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCCAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAACTCGACCAGTAGCTTGTTCAGGCGACGAACGt > 1:1336222/1‑150 (MQ=255) aGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTa < 1:40577/150‑1 (MQ=255) gACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTaa < 2:316803/150‑1 (MQ=255) aGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCgg < 1:738557/83‑1 (MQ=255) aGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCgg > 2:738557/1‑83 (MQ=255) gATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGc > 2:319322/1‑103 (MQ=255) gATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGc < 1:319322/103‑1 (MQ=255) aaCCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTc > 1:287500/1‑150 (MQ=255) gCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGCCACAATGAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACACCGCGATCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCa < 1:298480/150‑1 (MQ=255) acgaTTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTcc > 2:110758/1‑138 (MQ=255) acgaTTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTcc < 1:110758/138‑1 (MQ=255) acgaTTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACGCAACCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTACGCCGCCGCGGCCCTCAGGCtctcc > 2:95509/1‑149 (MQ=255) tttGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGgccgc > 1:1574136/1‑150 (MQ=255) cTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTCCTGTGCATCAACTAAAGCAGCCAGCTCGACCAGTAGCTTGTTCACGCGCCGATCGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAgg < 2:1421066/150‑1 (MQ=255) tcaAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACg < 1:1098811/107‑1 (MQ=255) tcaAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACg > 2:1098811/1‑107 (MQ=255) cTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGgcc < 2:50473/133‑1 (MQ=255) cTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGgcc > 1:50473/1‑133 (MQ=255) tCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCg > 2:968042/1‑150 (MQ=255) ttgagttgatgcacagcAAAGCCCGCTTCGGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGgccgc < 1:916317‑M1/122‑1 (MQ=255) ttgagttgatgcacagcAAAGCCCGCTTCGGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGgccgc > 2:916317‑M1/18‑139 (MQ=255) aCTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGa > 1:123366/1‑138 (MQ=255) aCTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGa < 2:123366/138‑1 (MQ=255) aCTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGAtg < 2:961486/150‑1 (MQ=255) tCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGAt > 2:426594/1‑144 (MQ=255) tCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGAt < 1:426594/144‑1 (MQ=255) tCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGATGTGCGa > 2:418010/1‑150 (MQ=255) acagcAAAGCCCGCTTCGGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGATGTGCGACa < 1:826095‑M1/145‑1 (MQ=255) cagcAAAGCCCGCTTCGGCGGGCTTTTCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCAcc < 1:1449951‑M1/107‑1 (MQ=255) cagcAAAGCCCGCTTCGGCGGGCTTTTCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCAcc > 2:1449951‑M1/5‑111 (MQ=255) agcAAAGCCCGCTTCGGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTg < 1:557725‑M1/63‑1 (MQ=255) agcAAAGCCCGCTTCGGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTg > 2:557725‑M1/4‑66 (MQ=255) aaaaaGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGATGTGCGACAGCt < 1:568339/150‑1 (MQ=255) cAAAGCCCGCTTCGGCGGGCTTTTCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACa < 2:1229815‑M1/133‑1 (MQ=255) AGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGATGTGCGACAGCTCGGc > 2:287500‑M1/1‑150 (MQ=255) GCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCgg < 2:399306‑M1/86‑1 (MQ=255) GCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCgg > 1:399306‑M1/1‑86 (MQ=255) cgAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTc > 2:450756/1‑87 (MQ=255) cgAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTc < 1:450756/87‑1 (MQ=255) | CCATCACCAGCCCTGGGCGGCCCAGCGCACCCGGCTCATAGACCAAACGGCTTTCGTAGGTCTTGCCAGACAGGTGATAAACCAGCGATTCGACGATTACTTTGCTCATCAAGCACTCCTTTGGCACTCGTCATAAAAAAGCCCGCCGAAGCGGGCTTTGCTGTGCATCAACTCAAGCAGACAGCTCGACCAGTAGCTTGTTCAGGCGACGAACGTAAGCCGCCGGGTCCTTCAGGCTGTCACCGGCCGCCAGGGCCGCCTGATCGAACAGGATGTGCGACAGCTCGGC > NC_002947/4721533‑4721821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |