Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,821,852 C→A 5.1% G3128V (GGC→GTC)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,821,8520CA 5.1% 248.4 / 3.0 78G3128V (GGC→GTC) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (41/33);  new base A (2/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

AGCGCAGTTGCAGAGCACCGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGATCGAACCGGCC  >  NC_002947/4821709‑4822001
                                                                                                                                               |                                                                                                                                                     
aGCGCAGTTGCAGAGCACCGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCTCGATTTGGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGt                                                                                                                                                 >  1:100014/1‑150 (MQ=255)
         gCAGAGCACCGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAgg                                                                                                                                        >  2:1303922/1‑150 (MQ=255)
           agagCACCGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTg                                                                                                                                      >  2:1427725/1‑150 (MQ=255)
           agagCACCGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTg                                                                                                                                      >  1:168406/1‑150 (MQ=255)
            gagCACCGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGa                                                                                                                                     >  2:301760/1‑150 (MQ=255)
                  cGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATgcgcg                                                                                                                               >  1:459362/1‑150 (MQ=255)
                      aTACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTg                                                                                                                           >  1:1150665/1‑150 (MQ=255)
                       tACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGc                                                                                                                          <  1:969816/150‑1 (MQ=255)
                            cTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGCACTGGCCCAGGTAGTTGAAgg                                                                                                                                        >  1:1320394/1‑131 (MQ=255)
                            cTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAgg                                                                                                                                        <  2:1320394/131‑1 (MQ=255)
                                          tCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCt                                                                                                       >  2:448798/1‑150 (MQ=255)
                                          tCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCt                                                                                                       <  2:1248410/150‑1 (MQ=255)
                                             aCCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTcgc                                                                                                    >  1:914898/1‑150 (MQ=255)
                                              ccAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGgc                                                                                                                  <  2:825888/135‑1 (MQ=255)
                                              ccAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGgc                                                                                                                  >  1:825888/1‑135 (MQ=255)
                                                  ttGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGTGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGATAGTTGAAGGTGATGCGCGCCTGCTGCAAGGCCGCCATCTGCTCGCGCCcg                                                                                               <  1:1025230/150‑1 (MQ=255)
                                                    gggCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATc                                                                                                           >  2:628023/1‑136 (MQ=255)
                                                    gggCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATc                                                                                                           >  2:628002/1‑136 (MQ=255)
                                                    gggCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATc                                                                                                           <  1:628023/136‑1 (MQ=255)
                                                    gggCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATc                                                                                                           <  1:628002/136‑1 (MQ=255)
                                                        aaCGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCa                                                                                         <  2:459362/150‑1 (MQ=255)
                                                           ggCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATcc                                                                                      >  2:732273/1‑150 (MQ=255)
                                                           ggCCCATCGAGTTCATGCGCCAACCCGGCCGAGGCCTCAAGAGGCCTGAACAAGTGCGCACTGTCGAACTGCTGATCTAACTTGCCCAGTTAGTTGAAGGGTATGCGCGCCTGCGGCAATACCGCCATCTGCTCGCGCCCGGCGGCATcc                                                                                      <  1:108875/150‑1 (MQ=255)
                                                              gcATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGcc                                                                                   >  2:1485917/1‑150 (MQ=255)
                                                                   gAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTa                                                                              >  2:534397/1‑150 (MQ=255)
                                                                         aTGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGt                                                                                                                                                 <  2:872187/77‑1 (MQ=255)
                                                                         aTGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGt                                                                                                                                                 >  1:872187/1‑77 (MQ=255)
                                                                         aTGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCa                                                                                  >  1:695857/1‑140 (MQ=255)
                                                                         aTGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCa                                                                                  <  2:695857/140‑1 (MQ=255)
                                                                             gcCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACg                                                                    >  2:382716/1‑150 (MQ=255)
                                                                               cAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGcc                                                                  >  2:53905/1‑150 (MQ=255)
                                                                                  cccGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTa                                                               >  1:1285542/1‑150 (MQ=255)
                                                                                  cccGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTa                                                               <  1:448798/150‑1 (MQ=255)
                                                                                   ccGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAGCTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAg                                                              >  2:1647735/1‑150 (MQ=255)
                                                                                   ccGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAg                                                              >  2:1393333/1‑150 (MQ=255)
                                                                                    cGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGc                                                             <  1:1119820/150‑1 (MQ=255)
                                                                                     ggCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGcc                                                            <  1:597364/150‑1 (MQ=255)
                                                                                      gCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGccc                                                           >  1:916712/1‑150 (MQ=255)
                                                                                          aGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGc                                                       <  1:53905/150‑1 (MQ=255)
                                                                                              cTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCtt                                                   <  1:534397/150‑1 (MQ=255)
                                                                                               tCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTtg                                                  >  1:769746/1‑150 (MQ=255)
                                                                                                ccAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTtgt                                                 <  2:1285542/150‑1 (MQ=255)
                                                                                                ccAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTtgt                                                 <  2:914898/150‑1 (MQ=255)
                                                                                                        tGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACa                                         >  1:1586983/1‑150 (MQ=255)
                                                                                                          gAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAg                                                              >  2:660203/1‑127 (MQ=255)
                                                                                                          gAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAg                                                              <  1:660203/127‑1 (MQ=255)
                                                                                                           aaCAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATgg                                      <  1:301760/150‑1 (MQ=255)
                                                                                                            aCAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAg                                                                        <  1:1469056/115‑1 (MQ=255)
                                                                                                            aCAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAg                                                                        >  2:1469056/1‑115 (MQ=255)
                                                                                                                 gCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGCTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCGgt                                >  2:347991/1‑150 (MQ=255)
                                                                                                                  ccGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAgtt                               <  2:916712/150‑1 (MQ=255)
                                                                                                                         gTCGAACTGCTGGGCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCtt                        <  2:258942/150‑1 (MQ=255)
                                                                                                                            gAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCa                                  >  2:6670/1‑137 (MQ=255)
                                                                                                                            gAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCa                                  <  1:6670/137‑1 (MQ=255)
                                                                                                                            gAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGAt                     >  1:1001118/1‑150 (MQ=255)
                                                                                                                             aaCTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGgccgc                                                                                                               <  2:523640/59‑1 (MQ=255)
                                                                                                                             aaCTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGgccgc                                                                                                               >  1:523640/1‑59 (MQ=255)
                                                                                                                                tgctgGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTa                                                               <  2:882471/104‑1 (MQ=255)
                                                                                                                                tgctgGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTa                                                               >  1:882471/1‑104 (MQ=255)
                                                                                                                                  ctgGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCtt               >  1:1013532/1‑150 (MQ=255)
                                                                                                                                  ctgGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAATACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCtt               >  1:575222/1‑150 (MQ=255)
                                                                                                                                    ggTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCTGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGa             <  2:1586983/150‑1 (MQ=255)
                                                                                                                                    ggTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGa             >  2:278762/1‑150 (MQ=255)
                                                                                                                                    ggTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGa             <  2:1150665/150‑1 (MQ=255)
                                                                                                                                     gTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGTAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGAt            >  2:1053572/1‑150 (MQ=255)
                                                                                                                                      tCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTcc                          <  2:1595403/135‑1 (MQ=255)
                                                                                                                                      tCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTcc                          >  1:1595403/1‑135 (MQ=255)
                                                                                                                                        gAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCt                                                                                                          <  2:118805/53‑1 (MQ=255)
                                                                                                                                        gAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCt                                                                                                          >  1:118805/1‑53 (MQ=255)
                                                                                                                                         atcTGGACCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGcc                                                                                   >  2:243514/3‑75 (MQ=255)
                                                                                                                                         atcTGGACCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGcc                                                                                   <  1:243535/73‑1 (MQ=255)
                                                                                                                                         atcTGGACCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGcc                                                                                   <  1:243514/73‑1 (MQ=255)
                                                                                                                                         atcTGGACCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGcc                                                                                   >  2:243535/3‑75 (MQ=255)
                                                                                                                                         aaCTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGATCGaa        <  2:1001118/150‑1 (MQ=255)
                                                                                                                                          aCTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGATCGAAc       >  1:145001/1‑150 (MQ=255)
                                                                                                                                            tGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCa                                                                                  >  2:449695/1‑73 (MQ=255)
                                                                                                                                            tGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCa                                                                                  <  1:449695/73‑1 (MQ=255)
                                                                                                                                             ggCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGATCGAACCgg    <  1:382716/150‑1 (MQ=255)
                                                                                                                                               cccAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGATCGAACCGGcc  >  1:847419/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
AGCGCAGTTGCAGAGCACCGCCATACACCTGGCCATCGACGCTCAACCAGTTGGGCAACGGCGCATCGAGGTCATGCGCCAACCCGGCCGAGGCCTCCAGGGGCTGGAACAAGGCCGCACTGTCGAACTGCTGGTCGAACTGGCCCAGGTAGTTGAAGGTGATGCGCGCCTGCGGCAAGGCCGCCATCTGCTCGCGCCCGGCGGCATCCGCCAGGTAGCGCAGTACGCCGTAGCCCAGGCCCTTGTGCGGTACATGGCGCAGTTGTTCCTTGATGCGCTTGATCGAACCGGCC  >  NC_002947/4821709‑4822001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑