Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,839,266 C→G 9.8% intergenic (+43/‑137) ccoP‑I → / → PP_4254 cbb3‑type cytochrome c oxidase subunit/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,839,2660CG 9.8% 120.2 / 5.8 41intergenic (+43/‑137)ccoP‑I/PP_4254cbb3‑type cytochrome c oxidase subunit/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (16/21);  new base G (2/2);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

ATGACAAGGTGCACCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCGCCGCCCCCTTCCCTTGCACCCCCACCGAACACAACTAAGCTTGTTGCCTCAGT  >  NC_002947/4839144‑4839404
                                                                                                                          |                                                                                                                                          
aTGACAAGGTGCACCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCCCCCGCGCGGGGCGCGATATCCGCCccacc                                                                                                                 >  2:380220/1‑150 (MQ=255)
 tGACAAGGTGCACCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCccacca                                                                                                                >  1:47022/1‑150 (MQ=255)
 tGACAAGGTGCACCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCccacca                                                                                                                >  2:885754/1‑150 (MQ=255)
   aCAAGGTGCACCTGCTGTCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCgcggc                                                                                                                                    >  2:694315/1‑128 (MQ=255)
   aCAAGGTGCACCTGCTGTCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCgcggc                                                                                                                                    <  1:694315/128‑1 (MQ=255)
     aaGGTGCACCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                              <  2:1263118/132‑1 (MQ=255)
     aaGGTGCACCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                              >  1:1263118/1‑132 (MQ=255)
            aCCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCCCGCCGATCTGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCaaa                                                                                                     <  1:100749/150‑1 (MQ=38)
                  tgGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTac                                                                                               >  2:154203/1‑150 (MQ=255)
                    gcggcTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGCAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATAACCGCCCCCCCCTCACCCTCAAACCtttccc                                                                                             >  2:708418/1‑146 (MQ=38)
                      ggcTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTCTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACAccc                                                                                           >  2:639405/1‑150 (MQ=255)
                          tACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGAATTTGCCGCCGGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGc                                                                                       <  2:874965/150‑1 (MQ=255)
                                     ccTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                              >  1:1024748/1‑100 (MQ=255)
                                     ccTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                              <  2:1024748/100‑1 (MQ=255)
                                     ccTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCccac                                                                                                                  >  1:483517/1‑112 (MQ=255)
                                     ccTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCccac                                                                                                                  <  2:483517/112‑1 (MQ=255)
                                     ccTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAAc                                                                                                            <  2:1195731/118‑1 (MQ=255)
                                     ccTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAAc                                                                                                            >  1:1195731/1‑118 (MQ=255)
                                                 tGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCaaa                                                                                                     >  1:65997/1‑113 (MQ=255)
                                                 tGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCaaa                                                                                                     <  2:65997/113‑1 (MQ=255)
                                                    ttCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATcccc                                                                           >  2:1238246/1‑136 (MQ=255)
                                                    ttCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATcccc                                                                           <  1:1238246/136‑1 (MQ=255)
                                                      cGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATAccccc                                                           <  1:127401/150‑1 (MQ=255)
                                                              gCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTc                                                                        >  2:1270146/1‑129 (MQ=255)
                                                              gCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCGTACACCCCCGCCCCACCATCCCCCTc                                                                        <  1:1270146/129‑1 (MQ=255)
                                                               cTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCgccgcc                                                  <  1:154203/150‑1 (MQ=255)
                                                                      ggCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAAc                                                                                                    <  1:628527/93‑1 (MQ=255)
                                                                      ggCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAAc                                                                                                    >  2:628527/1‑93 (MQ=255)
                                                                        ccAAGTGAGTTCGCCGAAAGGCATCTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCGCCGCCcccttccct                                         <  1:652273/150‑1 (MQ=255)
                                                                          aaGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATcccc                                                                           <  2:979270/114‑1 (MQ=255)
                                                                          aaGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATcccc                                                                           >  1:979270/1‑114 (MQ=255)
                                                                             tGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCGCCGCCCCCTTCCCTTGCAc                                    <  2:1103962/150‑1 (MQ=255)
                                                                                    gCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCTGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCGCCGCCCCCTTCCCTTGCACCCCCAcc                             <  2:878901/150‑1 (MQ=255)
                                                                                      cGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTa                                                                                                >  2:538859/1‑81 (MQ=255)
                                                                                      cGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTa                                                                                                <  1:538859/81‑1 (MQ=255)
                                                                                      cGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCccac                                                                                  <  2:67969/95‑1 (MQ=255)
                                                                                      cGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCccac                                                                                  >  1:67969/1‑95 (MQ=255)
                                                                                                 cgccgccTGTGAGATCGAGCGCCGCCCGGGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCTCCGCCGCCCCCTTCCCTTGCACCCCCACCGAACACAACTAAg                <  1:380220/149‑1 (MQ=255)
                                                                                                    cgccTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAGACCATACCCCCACCGCCGCCCCCTTCCCTTGCACCCCCACCGAACACAACTAAGCtt             <  1:708418/150‑1 (MQ=255)
                                                                                                       cTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCGCCGCCCCCTTCCCTTGCACCCCCACCGAACACAACTAAGCttgtt          <  2:47022/150‑1 (MQ=255)
                                                                                                               tCGCGCCCCCCCCCCGCGTCTCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCGCCGCCCCCTTCCCTTGCACCCCCACCGAACACAACTAAGCTTGTTGCCTCAGt  <  2:1117129/150‑1 (MQ=255)
                                                                                                                          |                                                                                                                                          
ATGACAAGGTGCACCTGCTGGCGGCTTACGTGTATAGCCTGTCGCAGGATGGTTCGGCTGAGGCTGTGACGGCCAAGTGAGTTCGCCGAAAGGCATTTGCCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATATCCGCCCCACCATAACCATCAAACCTTACACCCCCGCCCCACCATCCCCCTCAAACCATACCCCCACCGCCGCCCCCTTCCCTTGCACCCCCACCGAACACAACTAAGCTTGTTGCCTCAGT  >  NC_002947/4839144‑4839404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑