Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 407,407 G→C 8.1% P201A (CCG→GCG)  aceF ← AceF‑S‑acetyldihydrolipoate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947407,4070GC 8.1% 143.5 / 5.5 50P201A (CCG→GCG) aceFAceF‑S‑acetyldihydrolipoate
Reads supporting (aligned to +/- strand):  ref base G (19/26);  new base C (2/2);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

CCTGCGTGAACCTTGGCATTGCTGCCAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTGTCGGACTCCAGGGTA  >  NC_002947/407268‑407555
                                                                                                                                           |                                                                                                                                                    
ccTGCGTGAACCTTGGCATTGCTGCCAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGcc                                                                                                                                            >  1:633961/1‑150 (MQ=255)
          ccTTGGCATTGCTGCCAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCGCAGCCGCGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCAc                                                                                                                                  >  2:1315296/1‑150 (MQ=255)
            ttGGCATTGCTGCCAGCGGCGGGTGCGGTAGCAGGCGGTACAGGGACAGCAGCGGGGTTGGTAGCGGAAGGTGCGGCAGCCGGAGCATCCTCTGCCTTGACTGGTGCGGGAGCAGCAGACCTTGCCGGCGTCGCAACCTcggcggtaacc                                                                                                                                <  2:768537/150‑6 (MQ=255)
            ttGGCATTGCTGCCAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCAccc                                                                                                                                >  2:1058902/1‑150 (MQ=255)
                    gctgcCAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGNGCAGCANCCGGTGCCGGCGNGGCAGCCGGGGCGGCACCCGCCACttt                                                                                                                        <  1:1310727/150‑1 (MQ=255)
                         cAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGggc                                                                                                                                       >  2:973293/1‑130 (MQ=255)
                         cAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGggc                                                                                                                                       <  1:973293/130‑1 (MQ=255)
                          aGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCTTCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCtt                                                                                                                  >  1:834552/1‑150 (MQ=255)
                                      ggTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTc                                                                                                      <  1:642201/150‑1 (MQ=255)
                                        tAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGAAGAATCAGCCTTGGGTCTGGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGc                                                                                                    >  1:609142/1‑150 (MQ=255)
                                        tAGCAGCCGGTGCAGGAGCTGGAGCCGGGGCTGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTCCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGc                                                                                                    <  1:1318947/150‑1 (MQ=255)
                                                     aGGGGCAGCAGCCGGGGCGGCAGCGGAAGGTACGGTAGCCCGACCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTc                                                                                       <  1:364976/150‑1 (MQ=255)
                                                       gggCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAgg                                                                                                        >  1:145561/1‑131 (MQ=255)
                                                       gggCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAgg                                                                                                        <  2:145561/131‑1 (MQ=255)
                                                       gggCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGAc                                                                                          >  2:1298312/1‑145 (MQ=255)
                                                       gggCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGAc                                                                                          <  1:1298312/145‑1 (MQ=255)
                                                       gggCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGt                                                                                     >  1:1269497/1‑150 (MQ=255)
                                                              agcCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAg                                                                              >  2:168751/1‑150 (MQ=255)
                                                                 cGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCtt                                                                           <  2:1615696/150‑1 (MQ=255)
                                                                  ggggCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTcc                                                                                <  1:883340/144‑1 (MQ=255)
                                                                  ggggCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTcc                                                                                >  2:883340/1‑144 (MQ=255)
                                                                          agcggcagGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCAc                                                                  >  2:1402542/1‑150 (MQ=255)
                                                                           gcggcagGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGCCCCGGCTGCCgcgcc                                                                                                                                       >  1:527597/1‑76 (MQ=38)
                                                                           gcggcagGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGCCCCGGCTGCCgcgcc                                                                                                                                       <  2:527597/80‑5 (MQ=38)
                                                                                  gTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGa                                                          <  2:1530012/150‑1 (MQ=255)
                                                                                  gTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGa                                                          >  1:1356169/1‑150 (MQ=255)
                                                                                      ggCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCAGGGCCGTGGCCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCAc                                                      <  1:920295/150‑1 (MQ=255)
                                                                                            cGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATc                                                                                                           >  1:838141/1‑91 (MQ=255)
                                                                                            cGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATc                                                                                                           <  2:838141/91‑1 (MQ=255)
                                                                                                  agcctcagcctTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCgg                                          <  1:1058902/150‑1 (MQ=255)
                                                                                                      tcagcctTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGAc                                      >  2:512145/1‑150 (MQ=255)
                                                                                                          cctTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGa                                  >  1:864076/1‑150 (MQ=255)
                                                                                                          cctTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGa                                  >  2:815585/1‑150 (MQ=255)
                                                                                                          cctTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGa                                  <  1:642082/150‑1 (MQ=255)
                                                                                                           ctTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGAt                                 >  1:1254630/1‑150 (MQ=255)
                                                                                                                cTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCa                            <  2:385698/150‑1 (MQ=255)
                                                                                                                 tGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCAt                           <  2:1269497/150‑1 (MQ=255)
                                                                                                                     gCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAg                       <  2:609142/150‑1 (MQ=255)
                                                                                                                       gggAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCTCCTGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGTCTTCGTTTTCCAGCTTGCCCAGGACTTCTTGGACCACGGCGGCAGCCGGCGACGGGATTTCCATGGAGGc                     <  2:1356169/149‑1 (MQ=255)
                                                                                                                         gAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCt                   >  2:1646036/1‑150 (MQ=255)
                                                                                                                           gcagcagcCGGTGCCGGCGCGGCAGCGGAGTCGTCATCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGCTTTCCATGGAGGCCTTg                 <  2:834552/150‑1 (MQ=255)
                                                                                                                           gcagcagcCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTg                 <  1:1596604/150‑1 (MQ=255)
                                                                                                                             agcatcCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGAt                                                                                                            >  1:463127/1‑57 (MQ=255)
                                                                                                                             agcagcCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGAt                                                                                                            <  2:463127/57‑1 (MQ=255)
                                                                                                                             agcagcCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTGTc               <  1:512145/150‑1 (MQ=255)
                                                                                                                                agcCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGAACACCACTGTTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTGTCGGa            <  1:199885/150‑1 (MQ=255)
                                                                                                                                   gggTGCCGCCCCGGCTGCCGCGCCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAg                       >  2:807723/2‑136 (MQ=255)
                                                                                                                                   gggTGCCGCCCCGGCTGCCGCGCCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAg                       <  1:807723/135‑1 (MQ=255)
                                                                                                                                   cGGTGCTGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTGTCGGACTc         <  2:633961/150‑1 (MQ=255)
                                                                                                                                       gCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTGTCGGACTCCAgg     <  1:168751/150‑1 (MQ=255)
                                                                                                                                          ggCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTGTCGGACTCCAGGGTa  <  1:815585/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
CCTGCGTGAACCTTGGCATTGCTGCCAGCGGCCGGTGCGGTAGCAGCCGGTGCAGGGGCAGCAGCCGGGGCGGCAGCGGCAGGTGCGGCAGCCGGAGCAGCCTCAGCCTTGGCTGGTGCAGGAGCAGCAGCCGGTGCCGGCGCGGCAGCCGGGGCGGCACCCGCCACTTTCAGCTTGAAGATCAGGTCGCCAGTGCCGACTTCGTCTTCCAGCTTGCACAGCACTTCTTCGACCACGCCGGCAGCCGGCGACGGGATTTCCATGGAGGCCTTGTCGGACTCCAGGGTA  >  NC_002947/407268‑407555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑