Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,013,331	T→C	12.1%	V35A (GTG→GCG)	PP_5678 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,013,331	0	T	C	12.1%	169.1 / 11.1	66	V35A (GTG→GCG)	PP_5678	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base C (4/4);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

ACCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGAC  >  NC_002947/5013187‑5013473                                                                                                                                                 |                                                                                                                                               aCCCGAGCAGTGCAACAAGCCTCAGAGTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                           >  1:387911/1‑150 (MQ=255) aCCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                           >  1:387716/1‑150 (MQ=255) aCCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGa                                                                                                                                           <  1:1155172/150‑1 (MQ=255)     gAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGGCGTGCTgtggat                                                                                                                                       <  1:1483043/150‑6 (MQ=255)       gCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCgcgg                                                                                                                                     >  2:1437601/1‑150 (MQ=255)              aacaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGaa                                                                                                                              >  2:788598/1‑150 (MQ=255)               acaaGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgctgt                                                                                                                             <  1:750293/150‑5 (MQ=255)                     aacAGAGTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGACATCCACAGAACGACGTGCTGAGCGCGGGGTGGAAATGAGGc                                                                                                                       >  1:645246/3‑150 (MQ=255)                      tCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCt                                                                                                                      <  1:788598/150‑1 (MQ=255)                                gggAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCaaaa                                                                                                            >  1:513700/1‑150 (MQ=255)                                gggAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCaaaa                                                                                                            >  2:213802/1‑150 (MQ=255)                                gggAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCacggcg                                                                                                                                   <  1:881382/127‑1 (MQ=255)                                gggAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCacggcg                                                                                                                                   >  2:881382/1‑127 (MQ=255)                                       ccAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                     >  1:77601/1‑150 (MQ=255)                                            gatgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTa                                                                                                >  2:642446/1‑150 (MQ=255)                                            gatgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgctgt                                                                                                                             <  1:104014/121‑5 (MQ=255)                                            gatgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgctgt                                                                                                                             >  2:104014/1‑117 (MQ=255)                                            gatgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgctgt                                                                                                                             <  1:518281/121‑5 (MQ=255)                                            gatgatCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGCGCTCAGCACGGCGTgctgt                                                                                                                             >  2:518281/1‑117 (MQ=255)                                                 tCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTg                                                                                                                                 >  2:599918/1‑112 (MQ=255)                                                 tCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTg                                                                                                                                 <  1:599918/112‑1 (MQ=255)                                                 tCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAAc                                                                                           >  1:458811/1‑150 (MQ=255)                                                   aaaGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTc                                                                                         <  2:77601/150‑1 (MQ=255)                                                     agagTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCa                                                                                       >  1:724760/1‑150 (MQ=255)                                                       agTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATc                                                                                     <  1:1437601/150‑1 (MQ=255)                                                        gTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCAGCCACAGCACGCCGTGCTACCCTTTGCATTGGAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCt                                                                                    >  1:774461/1‑150 (MQ=255)                                                           gACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTGGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTg                                                                                 >  2:305805/1‑150 (MQ=255)                                                            aCCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGc                                                                                <  2:513700/150‑1 (MQ=255)                                                              ccTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCga                                                                              <  1:1556934/150‑1 (MQ=255)                                                                tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgcag                                                                                                                 >  1:304151/1‑113 (MQ=255)                                                                tCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCgcag                                                                                                                 <  2:304151/113‑1 (MQ=255)                                                                    aGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTg                                                                                                                                 <  2:1243369/93‑1 (MQ=255)                                                                    aGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTg                                                                                                                                 >  1:1243369/1‑93 (MQ=255)                                                                        gTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGcttc                                                                    >  1:448277/1‑150 (MQ=255)                                                                             ggTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGc                                                               <  1:642446/150‑1 (MQ=255)                                                                               tCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                     <  1:83788/110‑1 (MQ=255)                                                                               tCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGaa                                                                                                     >  2:83788/1‑110 (MQ=255)                                                                                                 gTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGc                                           <  1:213802/150‑1 (MQ=255)                                                                                                  ttGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCTGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCg                                          <  2:387911/150‑1 (MQ=255)                                                                                                  ttGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCGGAGCGCTGCGTGGAATTCAGGCGCGCAGGAAAACCCCGAAGACTATGAACTCCATCTTTGCGAGAGCTGGTTCTTTGCCACGCTTTCAACGATAAAGCg                                          <  2:387716/150‑1 (MQ=255)                                                                                                   tGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGt                                         <  2:448277/150‑1 (MQ=255)                                                                                                      ggCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCGTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGaa                                      >  1:177891/1‑150 (MQ=255)                                                                                                        ccAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGcttctt                                                                  >  1:1091019/1‑120 (MQ=255)                                                                                                        ccAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGcttctt                                                                  <  2:1091019/120‑1 (MQ=255)                                                                                                         cAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                   >  1:1286993/1‑150 (MQ=255)                                                                                                         cAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                   <  2:458811/150‑1 (MQ=255)                                                                                                         cAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGc                                   >  1:1390348/1‑150 (MQ=255)                                                                                                                 tCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAg                                            <  1:633142/133‑1 (MQ=255)                                                                                                                 tCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAg                                            >  2:633142/1‑133 (MQ=255)                                                                                                                    ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATaaa                                             >  2:1492466/1‑129 (MQ=255)                                                                                                                    ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATaaa                                             <  1:1492466/129‑1 (MQ=255)                                                                                                                    ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTggg                               <  2:1589664/143‑1 (MQ=255)                                                                                                                    ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTggg                               >  1:1589664/1‑143 (MQ=255)                                                                                                                    ttCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAAtt                        >  2:1033689/1‑150 (MQ=255)                                                                                                                        gatgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATg                    >  1:208316/1‑150 (MQ=255)                                                                                                                         atgaCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGt                   >  2:1115862/1‑150 (MQ=255)                                                                                                                            aCGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTtct                <  2:774461/150‑1 (MQ=255)                                                                                                                                ccATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTGGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGa            <  1:305805/150‑1 (MQ=255)                                                                                                                                ccATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGa            <  1:1115862/150‑1 (MQ=255)                                                                                                                                     cacaGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAg       >  2:1461069/1‑150 (MQ=255)                                                                                                                                       caGCACGCCGTGCTGAGCGCGTCGTGGAAATCAGGCGCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGGGAACGCTGGGCCCAATTGCTGTTCTCTGAGGAAGCt     <  2:839730/150‑1 (MQ=255)                                                                                                                                       caGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCt     <  2:1390348/150‑1 (MQ=255)                                                                                                                                       caGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCt     <  1:1461069/150‑1 (MQ=255)                                                                                                                                       caGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCt     <  1:1033689/150‑1 (MQ=255)                                                                                                                                         gCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGa   >  1:1200010/1‑150 (MQ=255)                                                                                                                                          cACGCCGCGCTCAGCACGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGAc  >  1:1571032/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                               ACCCGAGCAGTGCAACAAGCCTCAGACTTAGGGGAGAGCCAATGATGATCAAAGAGTCGACCCTCGCAGCCGTTGCGGTCAGCGTAGTTTGCGACCGTTGCGGCCAGACTTGTCCTTCTGATGACGGCCATCCACAGCACGCCGTGCTGAGCGCGGCGTGGAAATCAGGCTCGCAGCAAAACCCCGAAGCCTATGAACTCCATCTTTGCGAGAGCTGCTTCTTTGCCACGCTTTCAACGATAAAGCGTGAACGCTGGGCCCAATTGATGTTCTCTGAGGAAGCTGAC  >  NC_002947/5013187‑5013473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑