| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,173,299 | G→A | 11.3% | intergenic (‑292/+40) | fadD‑I ← / ← fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,173,299 | 0 | G | A | 11.3% | 132.1 / 7.9 | 53 | intergenic (‑292/+40) | fadD‑I/fadD‑II | long‑chain‑fatty acid‑‑CoA ligase/long‑chain‑fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base G (20/27); new base A (2/4); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
CAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTTGGCC > NC_002947/5173150‑5173447 | cAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAg > 1:874408/1‑150 (MQ=255) aaaCAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAgtg < 1:128412/150‑1 (MQ=255) aaCAGGCATCATAGGCACAAAAATGACTCAATGAACAATACAGGTCATTTTTCCAGTTCCCGAACCGAGA‑GTCTCGTATAAGGCTATATAGATCATGTTAGCGCCGTTTAAGGCATTCGCGGGTAATCCCGCTCCTACATTGGGCAgtgt < 1:1606702/150‑1 (MQ=255) cagtctttATGTACACAAAAATGACTCACTGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAg < 2:267648/144‑1 (MQ=255) ggCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGa < 2:1541022/150‑1 (MQ=255) gCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAg > 1:96272/1‑150 (MQ=255) ccTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACACTGCCCAATGTAGGAGc < 1:865437/150‑1 (MQ=39) cTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACACTGCCCAATGTAGGAGCg < 1:1405351/150‑1 (MQ=39) ttATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACACTGCCCAATGTAGGAGCgg < 1:1079046/150‑1 (MQ=38) ttATAGACATAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCgg < 2:935902/150‑1 (MQ=255) tATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCggg < 2:1553903/150‑1 (MQ=255) tATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCggg < 2:72327/150‑1 (MQ=255) aTGGTCACGAAAATGACTTAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGt < 2:1613189/150‑1 (MQ=255) aCTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGaagaag < 2:96272/150‑1 (MQ=255) cTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGc < 2:874408/150‑1 (MQ=255) tGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTCTACGGATTCGCGGGTAAACCCGCTCATCAACTGGCCAGTGTACTTCGGACATTACCCTCGAAGAAGCCATcc > 1:283492/1‑150 (MQ=255) aaCAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAgtgt < 1:1017347/117‑1 (MQ=255) aaCAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAgtgt > 2:1017347/1‑117 (MQ=255) tACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCggg > 2:839942/1‑121 (MQ=255) tACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCggg < 1:839942/121‑1 (MQ=255) tACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATc > 1:293352/1‑150 (MQ=255) gTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCt > 1:990029/1‑150 (MQ=255) gTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCt < 1:1258518/150‑1 (MQ=255) tCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCta < 1:1279649/150‑1 (MQ=255) ttttAGAG‑TACTTATCCAAACTGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATGCGCAGGTAAACCCCCTCTTACATTGGGCAGTGTAGGAGCCGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATc < 2:115735/150‑1 (MQ=255) aTTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTCACCCGCGACGAAGCCATCCCGGTATCACGCTATATCGCGCAACTc‑c > 2:425817/1‑150 (MQ=255) ttATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCaa > 1:953571/1‑145 (MQ=255) ttATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCaa < 2:953571/145‑1 (MQ=255) cAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCgg > 1:525660/1‑94 (MQ=255) cAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCgg < 2:525660/94‑1 (MQ=255) aGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGcc > 2:719637/1‑150 (MQ=255) aGAAAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCGGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGcc < 1:122790/150‑1 (MQ=255) gatctcTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATgg < 1:1419443/145‑1 (MQ=255) agagCCTGTTAGAGCGGTTTAAGCCATTCGCGGGTAAACCCGCTCCTCCACTTGGCAGTGTAGGAGCGGGTTTACCGGCGAAGAAGCCATCCTGGTATCACGCTATATCGCGCAACTCGCTGCCG‑AAAATCTTGCCCACCGGCGTCATgg < 1:42201/150‑1 (MQ=38) atccTGTTAGAGCGTTTTAAGGCATTCGCAGGTAAACCCGCTCCTACATTGGTCGGTGTAGTAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGc < 2:283492/148‑1 (MQ=39) tGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCAGCGAAGAAGCCATCCCGGTATCACGCTAGAGCGCTCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATgggagatc > 2:1419443/1‑145 (MQ=39) aGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGa < 2:990029/150‑1 (MQ=255) gcgGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTg < 1:719637/150‑1 (MQ=255) gTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTa > 2:516935/1‑150 (MQ=255) acacGCTCCTACATTGGGCAGTGTAGGAGCTGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACttt > 2:515936/4‑150 (MQ=255) ccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCgcagca < 1:340469/129‑1 (MQ=255) ccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCgcagca > 2:340469/1‑129 (MQ=255) ccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCtt > 1:1523520/1‑139 (MQ=255) ccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCtt < 2:1523520/139‑1 (MQ=255) ccGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGt > 2:894752/1‑150 (MQ=255) ccGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAATAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGAtgtg < 1:472116/136‑1 (MQ=38) ccGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAATAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGAtgtg > 2:472116/1‑136 (MQ=38) ccGCTCCTACACTGCCCAATGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGt > 1:1459962/1‑150 (MQ=38) cGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTa < 1:894752/150‑1 (MQ=255) gCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATgg < 1:1443600/109‑1 (MQ=255) gCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATgg > 2:1443600/1‑109 (MQ=255) aTTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTg < 2:1613733/139‑1 (MQ=255) aTTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTg > 1:1613733/1‑139 (MQ=255) ttGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTc > 1:1482031/1‑110 (MQ=255) ttGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTc < 2:1482031/110‑1 (MQ=255) ttGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAg > 2:1230816/1‑150 (MQ=255) gCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTTgg > 2:302625/1‑150 (MQ=255) aGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTTGGcc < 1:350993/150‑1 (MQ=255) | CAAAACAGGCCTTATGGTCACAAAAATGACTCAATGAACAATACAGGTCATTTTTAGAG‑TAATTATCCAAAATGTCGCAGATAAGGCTGTATAGAGCCTGTTAGAGCGGTTTAAGGCATTCGCGGGTAAACCCGCTCCTACATTGGGCAGTGTAGGAGCGGGTTTACCCGCGAAGAAGCCATCCCGGTATCACGCTATATCGCGCAACTC‑CCGCCGCAAAATCTTGCCCACCGGCGTCATGGGCAACGATTCGCGCAGCACGATGTGCTTGGGTACTTTGTAGCCGGTGAAGTTGGCC > NC_002947/5173150‑5173447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |