| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,222,953 | G→C | 61.5% | intergenic (‑316/+63) | PP_4602 ← / ← PP_4603 | AraC family transcriptional regulator/transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,222,953 | 0 | G | C | 61.5% | 3.1 / 12.2 | 13 | intergenic (‑316/+63) | PP_4602/PP_4603 | AraC family transcriptional regulator/transposase |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base C (5/3); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01 | |||||||||||
TTTCCCGCGCATTGGGAACCATAGCAAAGTATTCGGCGGCTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGG > NC_002947/5222821‑5223092 | gagttcgaagccagaagccaagaccggcaggtccgggctttgcttcgcaaagcccggaaactggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCCCAGCCCGGAAGGGCTgg > 2:1131223‑M1/132‑150 (MQ=255) agttcgaagccagaagccaagaccggcaggtccgggctttgcttcgcaaagcccggaaactggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCCCAGCCCGGAAGGGCTggg > 2:1537833‑M1/131‑150 (MQ=255) agaagccaagaccggcaggtccgggctttgcttcgcaaagcccggaaactggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgctgtgcgacagCGCAGCCCGGAAGGGCTGGGTGATCTCtcat < 1:146898‑M1/31‑1 (MQ=255) gaagccaagaccggcaggtccgggctttgcttcgcaaagcccggaaactggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCCCAGCCCGGAAg < 1:1469375‑M1/13‑1 (MQ=255) gaagccaagaccggcaggtccgggctttgcttcgcaaagcccggaaactggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCCCAGCCCGGAAg > 2:1469375‑M1/119‑131 (MQ=255) ccaagaccggcaggtccgggctttgcttcgcaaagcccggaaactggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgctgtgcgacagCGCAGCCCGGAAGGGCTGGGTGATCTCTCATcaaac > 2:146898‑M1/115‑150 (MQ=255) cgggcttggcgaagcaaagcccggaaactggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCGCAGCCCGGAAGGGCTGGGTGATCTCtcatc > 2:714785‑M1/99‑130 (MQ=255) ggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCGCAGCCCGGAAGGGCTGGGTGAtc < 1:20353‑M1/25‑1 (MQ=255) ggggtatcaactggtggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCGCAGCCCGGAAGGGCTGGGTGAtc > 2:20353‑M1/70‑94 (MQ=255) ggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCCCAGCCCGGAAGGGCTGGGt < 1:1070503‑M1/21‑1 (MQ=255) ggccgcttgagtgcttagaaaacaatgggttgcattttgtttgatgagagatcaCCCAGCCCGGAAGGGCTGGGt > 2:1070503‑M1/55‑75 (MQ=255) gatgagagatcaCCCAGCCCTTCCGGGCTGCGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCTTCGCAAAGCCCg > 1:714785‑M1/13‑130 (MQ=255) gagagatcaCCCAGCCCTTCCGGGCTGCGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTgg < 1:275861‑M1/141‑1 (MQ=255) | TTTCCCGCGCATTGGGAACCATAGCAAAGTATTCGGCGGCTGACCTACCCGGATCAGTCGGCGAATGCTTTTCTAGATCTCGATGTCAGTGCAGATCTGACGTCATGCGCATCCTGTTTGCTGTGCGACAGCGCAGCCCGGAAGGGCTGGGTGATCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGG > NC_002947/5222821‑5223092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |