Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,381,316 T→A 5.4% intergenic (+33/+32) bamE → / ← PP_4732 outer membrane protein assembly factor BamE/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,381,3160TA 5.4% 226.7 / 4.1 75intergenic (+33/+32)bamE/PP_4732outer membrane protein assembly factor BamE/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/34);  new base A (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

CGCAACCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAA  >  NC_002947/5381168‑5381458
                                                                                                                                                    |                                                                                                                                              
cgcAACCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTATGCGGGAACATCAGATGCAAAAAAGCCCGGACTTg                                                                                                                                               <  1:386282/150‑1 (MQ=255)
   aaCCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTc                                                                                                                                            <  2:903913/150‑1 (MQ=255)
       tGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCggg                                                                                                                                        >  1:397126/1‑150 (MQ=255)
        gaagaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGc                                                                                                                                       >  2:771294/1‑150 (MQ=255)
          agaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCtt                                                                                                                                     <  1:771294/150‑1 (MQ=255)
           gaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCGGGGCttt                                                                                                                                    <  2:1495807/150‑1 (MQ=255)
           gaaAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCGGGTCCGGGCttt                                                                                                                                    >  1:745550/1‑150 (MQ=255)
               aaCCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTtttg                                                                                                                                <  1:1050021/150‑1 (MQ=255)
                 ccGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTtttgtt                                                                                                                              >  2:575015/1‑150 (MQ=255)
                             ggTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGTACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATAc                                                                                                                  <  1:1205933/150‑1 (MQ=255)
                              gTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTc                                                                                                                                            <  1:1460385/123‑1 (MQ=255)
                              gTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTc                                                                                                                                            >  2:1460385/1‑123 (MQ=255)
                              gTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACg                                                                                                                 >  1:1214337/1‑150 (MQ=255)
                                 cGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCa                                                                                                              <  1:585688/150‑1 (MQ=255)
                                  gACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAg                                                                                                             <  1:1603991/150‑1 (MQ=255)
                                       aaGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCAtt                                                                                                        >  2:1041497/1‑150 (MQ=255)
                                       aaGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCAtt                                                                                                        <  1:1041497/150‑1 (MQ=255)
                                       aaGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGCACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCAtt                                                                                                        >  2:503374/1‑150 (MQ=255)
                                           gTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAt                                                                                                    >  1:375506/1‑150 (MQ=255)
                                           gTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAAt                                                                                                    >  2:346683/1‑150 (MQ=255)
                                               atccaGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCTTTtttgcat                                                                                                                             <  2:1100258/121‑4 (MQ=38)
                                               atccaGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCTTTtttgcat                                                                                                                             >  1:1100258/1‑118 (MQ=38)
                                                        gagaTCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCt                                                                                       <  1:575015/150‑1 (MQ=255)
                                                            tCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCCGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCa                                                                                   >  2:485461/1‑150 (MQ=255)
                                                               acacCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTta                                                                                                                     <  2:65492/113‑1 (MQ=255)
                                                               acacCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTta                                                                                                                     >  1:65492/1‑113 (MQ=255)
                                                                acccATCGAGACTCCCCCGGTCCCGACCCCGGCACCGCTGGAAACCGGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAAc                                                                               <  2:20057/148‑1 (MQ=255)
                                                                    aTCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCg                                                                                                                                          <  2:907352/87‑1 (MQ=255)
                                                                    aTCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCg                                                                                                                                          >  1:907352/1‑87 (MQ=255)
                                                                     tCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGTCAc                                                                                          <  1:1565417/134‑1 (MQ=255)
                                                                     tCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATc                                                                          >  1:1332793/1‑150 (MQ=255)
                                                                     tCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCAGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGTCAc                                                                                          >  2:1565417/1‑134 (MQ=255)
                                                                       gagaCTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGa                                                                        >  1:461210/1‑150 (MQ=255)
                                                                        agaCTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGAc                                                                       <  1:503374/150‑1 (MQ=255)
                                                                        agaCTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGAc                                                                       >  2:741506/1‑150 (MQ=255)
                                                                         gaCTACCCCGGTCCCGACCCCGGCACCGCTAGATACCCGCTAAGCGGCACCCTCAGATGTAAAAAGCGTCACGCTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGAcc                                                                      <  1:1264084/150‑1 (MQ=255)
                                                                            tACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGc                                                                   <  2:397126/150‑1 (MQ=255)
                                                                              ccccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGt                                                                 >  1:525382/1‑150 (MQ=255)
                                                                               cccGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTa                                                                >  1:1142156/1‑150 (MQ=255)
                                                                                 cGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCa                                                                                           <  2:393689/121‑1 (MQ=255)
                                                                                 cGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCa                                                                                           >  1:393689/1‑121 (MQ=255)
                                                                                      ccGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCttt                                                                                                                                    >  1:1331477/1‑75 (MQ=37)
                                                                                      ccGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACCAAGTCCGGGCttt                                                                                                                                    <  2:1331477/75‑1 (MQ=37)
                                                                                       cGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTcgccgc                                                        >  1:1406634/1‑150 (MQ=255)
                                                                                           cccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCCATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGa                                                    <  2:1214337/150‑1 (MQ=255)
                                                                                            ccGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGAt                                                   <  2:525382/150‑1 (MQ=255)
                                                                                             cGGCACCGCTGGAAACCCGCTAAGCGGGAACATCCGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGCCAGGCATTCAATTGCTTGGCCCGCTGGCAGAACACATCTACCAGCGTATTCggcgccgcttt                                                  >  1:1510202/1‑145 (MQ=255)
                                                                                                             ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGaa                                               >  1:135581/1‑137 (MQ=255)
                                                                                                             ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGaa                                               <  2:135581/137‑1 (MQ=255)
                                                                                                             ccGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAAc                                  <  1:485461/150‑1 (MQ=255)
                                                                                                               gCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGt                                >  2:1387550/1‑150 (MQ=255)
                                                                                                               gCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGt                                >  2:384578/1‑150 (MQ=255)
                                                                                                                cTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTg                               <  2:1332793/150‑1 (MQ=255)
                                                                                                                    gCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCggg                                        <  2:1433263/137‑1 (MQ=255)
                                                                                                                    gCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCATTATACGTCAGGCATTCAATGGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCggg                                        >  1:1433263/1‑137 (MQ=255)
                                                                                                                     cGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGgcgcg                          <  2:461210/150‑1 (MQ=255)
                                                                                                                          aCATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCg                                          <  1:803407/129‑1 (MQ=255)
                                                                                                                          aCATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCg                                          >  2:803407/1‑129 (MQ=255)
                                                                                                                               aGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTc                                                             >  2:855234/1‑105 (MQ=255)
                                                                                                                               aGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTc                                                             <  1:855234/105‑1 (MQ=255)
                                                                                                                                  tGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCg             >  1:425816/1‑150 (MQ=255)
                                                                                                                                   gCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATAc                                                                                                                  <  2:1613184/48‑1 (MQ=255)
                                                                                                                                   gCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATAc                                                                                                                  >  1:1613184/1‑48 (MQ=255)
                                                                                                                                     aaaaaaGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCg          >  2:1029396/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCg          >  1:1357795/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCg          >  1:1274701/1‑150 (MQ=255)
                                                                                                                                          aGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGccc              >  2:1386389/1‑141 (MQ=255)
                                                                                                                                          aGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGccc              <  1:1386389/141‑1 (MQ=255)
                                                                                                                                           gCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGaa                                                                                <  2:381303/74‑1 (MQ=255)
                                                                                                                                           gCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGaa                                                                                >  1:381303/1‑74 (MQ=255)
                                                                                                                                             ccGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGAc                                                                       <  1:716454/81‑1 (MQ=255)
                                                                                                                                             ccGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGAc                                                                       >  2:716454/1‑81 (MQ=255)
                                                                                                                                             ccGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGaa  <  2:1274701/150‑1 (MQ=255)
                                                                                                                                               ggACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATa                                             >  1:958267/1‑105 (MQ=255)
                                                                                                                                               ggACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATa                                             <  2:958267/105‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
CGCAACCTGAAGAAAAACCGGCCAAGCCTGGTTCGACCGAAGAGTCCATCCAGCGCGAGATCGACACCATCGAGACTACCCCGGTCCCGACCCCGGCACCGCTGGAAACCCGCTAAGCGGGAACATCAGATGCAAAAAAGCCCGGACTTGGTCCGGGCTTTTTTGTTTGCGCCTTATACGTCAGGCATTCAATTGCTTGGCACGCTGGCAGAACGCATCGACCAGCGTATTCGCCGCCTGATTGAATAGCGGGCCCAACGTGGCGCGCACAATCGGCCCGGCGTAATCGAA  >  NC_002947/5381168‑5381458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑