| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,531,058 | A→C | 7.4% | R421R (CGT→CGG) | braE ← | high‑affinity branched‑chain amino acid ABC transporter permease BraE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,531,058 | 0 | A | C | 7.4% | 191.8 / 5.3 | 67 | R421R (CGT→CGG) | braE | high‑affinity branched‑chain amino acid ABC transporter permease BraE |
| Reads supporting (aligned to +/- strand): ref base A (31/31); new base C (2/3); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GGCAAATATCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACACCCAC > NC_002947/5530909‑5531192 | ggCAAATATCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATa > 1:1161584/1‑150 (MQ=255)ggCAAATATCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCAATCCTTTACGTGGGGTCACACCGGTACGGCTGATa > 1:769592/1‑150 (MQ=255) aaaTATCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACgg > 2:884775/1‑150 (MQ=255) aaaTATCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACgg > 2:884611/1‑150 (MQ=255) atatCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGAt < 2:1408597/150‑1 (MQ=255) tatCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATc < 1:884611/150‑1 (MQ=255) tatCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATc < 1:884775/150‑1 (MQ=255) gcgcCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATCCGTATCAGccgtacc < 1:1362946/150‑6 (MQ=255) cgcCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCAcgcg < 1:1007899/150‑1 (MQ=255) gcCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGTTACGGCTGATACGGATCAGCCCACGCgg > 1:771824/1‑150 (MQ=255) gACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGc < 2:1161584/150‑1 (MQ=255) aGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTc < 2:634016/141‑1 (MQ=255) aGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTc > 1:634016/1‑141 (MQ=255) gCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGatcatc > 1:1332462/1‑150 (MQ=255) cTGACGGCGGTGAGCGCCTTGATGCCACCGAACGTCATCATCAGGTTGTCGACCGAGAGAATGATATCTTCGCGCATGGCGCCACTCCTTTACGTCGTGTCACACCGGTACTGCTGATACGGATCAGCCCACGCGGTCGCCAGatcatca < 2:534179/150‑1 (MQ=255) gTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGTCCACGCGGTCGCCAGatc < 2:238512/141‑1 (MQ=255) gTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGTCCACGCGGTCGCCAGatc > 1:238512/1‑141 (MQ=255) gTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCAcgc > 2:554353/1‑129 (MQ=255) gTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCAcgc < 1:554353/129‑1 (MQ=255) cgcCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATcatcagcaccatcagc < 1:1469604/150‑1 (MQ=255) gcCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATcatcagcaccatcagca > 2:617902/1‑150 (MQ=255) cTTGATGCCACCGAACTGCATATTCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGccc > 2:1089933/1‑113 (MQ=255) cTTGATGCCACCGAACTGCATACTCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGccc < 1:1089933/113‑1 (MQ=255) tGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCCCTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATcatcagcaccatcagc < 1:112177/142‑1 (MQ=255) tGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGACACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATcatcagcaccatcagc > 2:112177/1‑142 (MQ=255) aCCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGccc > 1:256338/1‑104 (MQ=255) aCCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGccc < 2:256338/104‑1 (MQ=255) cGAACTGCATCATCAGGTTGTCGACCGAGAAAGCGATGTCGTCGCTGGTTGCGGCACTCCTTTACGTGGGCTCAAACAGGTCGTACTGAGACAGTGGATCCCACGCGGTCGCCAGATCATCATCAGCACCATCACCACGCCGAAcagcag > 1:372851/1‑150 (MQ=255) aaCTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAgcagca < 2:1332462/150‑1 (MQ=255) cTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCAcc > 1:353022/1‑150 (MQ=255) catcatCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCgg > 1:872511/1‑150 (MQ=255) tcaGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTc < 2:771824/150‑1 (MQ=255) ggTTTTCGACCCAGATAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGga < 2:769592/150‑1 (MQ=255) gTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGc > 2:1479990/1‑124 (MQ=255) gTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGc < 1:1479990/124‑1 (MQ=255) aCCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGaa > 2:152952/1‑121 (MQ=255) aCCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGaa < 1:152952/121‑1 (MQ=255) aaTGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATCCgt > 1:1566337/1‑62 (MQ=255) aaTGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATCCgt < 2:1566337/63‑2 (MQ=255) tatCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCgg > 1:230273/1‑150 (MQ=255) aTGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCa < 2:372851/150‑1 (MQ=255) cgcCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCAc < 1:1421964/150‑1 (MQ=255) cccTCCTTTACGTGGGGTCCCCACGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGaa < 2:353022/148‑1 (MQ=255) aCTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCAc > 2:1066412/1‑146 (MQ=255) aCTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCAc < 1:1066412/146‑1 (MQ=255) tCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAAcgc > 1:1034159/1‑150 (MQ=255) cTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCgcagc > 1:625432/1‑119 (MQ=255) cTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCgcagc < 2:625432/119‑1 (MQ=255) ggggTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGAt > 1:1555556/1‑146 (MQ=255) ggggTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGAt < 2:1555556/146‑1 (MQ=255) gTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACGATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACcaca > 2:1345247/1‑150 (MQ=255) acacCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAg < 1:605416/96‑1 (MQ=255) acacCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAg > 2:605416/1‑96 (MQ=255) acacCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACAccc > 1:126865/1‑150 (MQ=255) acacCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACAccc > 1:119078/1‑150 (MQ=255) acCGGTACGGCTGATACGGATCAGTCCACGCGGTCGCCAGATCATcatcagcaccat > 2:213194/1‑57 (MQ=255) acCGGTACGGCTGATACGGATCAGTCCACGCGGTCGCCAGATCATcatcagcaccat < 1:213194/57‑1 (MQ=255) acCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAGCGCCGCGATGACCACACCCAc > 1:1536843/1‑150 (MQ=255) acCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACACCCAc < 2:309435/150‑1 (MQ=255) acCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACACCCAc < 2:1034159/150‑1 (MQ=255) acCGGTACAGCTGATACGGATCAGCCCACGCGGACGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCAGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACACCCAc > 2:321592/1‑150 (MQ=255) gTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGAc > 2:812856/1‑137 (MQ=255) gTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGAc < 1:812856/137‑1 (MQ=255) tACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGa > 2:452887/1‑135 (MQ=255) tACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGa < 1:452887/135‑1 (MQ=255) gCTGATCCGTATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCgca < 2:459254/92‑1 (MQ=255) gCTGATCCGTATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCgca > 1:459254/1‑92 (MQ=255) tGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAAca < 2:376100/60‑1 (MQ=255) tGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAAca > 1:376100/1‑60 (MQ=255) | GGCAAATATCTGGTTGCGCCGGACCTTCAGGCTGACGTCGCTGAGCGCCTTGATGCCACCGAACTGCATCATCAGGTTGTCGACCGAGAGAATGATATCGTCGCTCATGGCGCCACTCCTTTACGTGGGGTCACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACACCCAC > NC_002947/5530909‑5531192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |