| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,549,653 | T→A | 6.3% | intergenic (+18/‑154) | PP_t47 → / → PP_4881 | tRNA‑Leu/iron ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,549,653 | 0 | T | A | 6.3% | 187.9 / 2.2 | 59 | intergenic (+18/‑154) | PP_t47/PP_4881 | tRNA‑Leu/iron ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (28/27); new base A (2/2); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAGGAAGT > NC_002947/5549510‑5549800 | gCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACACAATAGCTTGCttt > 2:1350599/1‑148 (MQ=255) cGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGt > 2:1146080/1‑150 (MQ=255) gAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTg > 2:3193/1‑150 (MQ=255) gAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCAAGCTAGTg < 1:277493/150‑1 (MQ=255) aaCGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTgg < 2:1244611/150‑1 (MQ=255) tCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTc > 2:1566304/1‑150 (MQ=255) gCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCtt < 1:616870/150‑1 (MQ=255) cAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGa > 1:1607510/1‑150 (MQ=255) tgatgTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTa < 1:1462599/150‑1 (MQ=255) gatgTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTAt < 2:469004/150‑1 (MQ=255) tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTggg < 2:832317/124‑1 (MQ=255) tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTggg > 1:832317/1‑124 (MQ=255) tAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGc > 2:1195454/1‑150 (MQ=255) aaaGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAAGCGCCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGcc > 1:935854/1‑150 (MQ=255) aaGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGccc < 2:848043/150‑1 (MQ=255) aaGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGccc > 1:806254/1‑150 (MQ=255) aaGCTTTGCCCAGGTGGCGGAATTCACACTGAAGTTGATTTAAGGTAGGAGTGACCGCAAGGGCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGccc > 2:837513/1‑150 (MQ=25) cccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTccccc > 2:878126/1‑150 (MQ=255) ccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGt < 2:778188/110‑1 (MQ=255) ccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGt > 1:778188/1‑110 (MQ=255) ccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGAttt > 2:1063425/1‑133 (MQ=255) ccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGAttt < 1:1063425/133‑1 (MQ=255) ccAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTcccccc < 1:3193/150‑1 (MQ=255) gAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCAt < 2:1607510/150‑1 (MQ=255) gAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCAt > 1:1454336/1‑150 (MQ=255) aaTTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATg < 2:1454336/150‑1 (MQ=255) gTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTgg > 1:489598/1‑96 (MQ=255) gTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTgg < 2:489598/96‑1 (MQ=255) aCGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTAGAGTCTTCTCCGGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGTCCa > 1:778820/1‑150 (MQ=255) cgcgCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCAt < 2:1000364/150‑1 (MQ=255) gTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTACAGTCAATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTtc < 1:1350599/150‑1 (MQ=37) tttCAGGCATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTtct < 2:871724/150‑1 (MQ=255) gTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTcac > 2:538598/1‑150 (MQ=255) tCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCt < 2:616906/65‑1 (MQ=255) tCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCt > 1:616906/1‑65 (MQ=255) cAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACgg < 2:806254/150‑1 (MQ=255) cGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTg > 2:599068/1‑150 (MQ=255) gCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCAACTAGAATGCTAGTGCGTTTTTCCATGCCTGGGATTGATTGGCCCCCTCCCTTCCTTGTACCTTGGTCTGACCATGAATTTCTCGTCACACGGCCACTTga > 2:1419218/1‑150 (MQ=37) ggTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTACACCATGGTCTGCCTGTAAATTTCTCGTCACCCCGCCACTTGAGaaa > 1:259558/1‑150 (MQ=255) gTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCtt > 1:1039029/1‑70 (MQ=255) gTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCtt < 2:1039029/70‑1 (MQ=255) tCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACa < 1:1146080/150‑1 (MQ=255) ggAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAAttt > 1:762769/1‑150 (MQ=255) ttCGAGTCTTCTCCTGGGCACCATACAAAAACCAACTAGCTTGCTAGTGGGTTTTTTCTTACCTGCGATTTATGTGCTCCCTCCCACCCTTGCTCCATGTTCTGAACATGAATTTCTCGTCACACGGCCACTTGAGAAAAAATTTCGttt > 2:117353/1‑150 (MQ=255) tcttctCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCAtt > 1:687796/1‑150 (MQ=255) tcCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAAttt > 1:1217661/1‑134 (MQ=255) tcCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAAttt < 2:1217661/134‑1 (MQ=255) gggCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTcaca < 1:1373145/109‑1 (MQ=255) gggCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTcaca > 2:1373145/1‑109 (MQ=255) gggCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCaaa < 1:995827/150‑1 (MQ=255) cATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTa < 1:1566304/150‑1 (MQ=255) tACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTCCCAAATATGTAGc < 1:1195454/150‑1 (MQ=255) aaaCCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGcc > 2:1557729/1‑50 (MQ=255) aaaCCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGcc < 1:1557729/50‑1 (MQ=255) aCCCACTAGCAAGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGa < 2:498716/75‑1 (MQ=38) aCCCACTAGCAAGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGa > 1:498716/1‑75 (MQ=38) cccACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCg > 1:1460601/1‑150 (MQ=255) ccACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCACTTCACACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGa < 1:837513/150‑1 (MQ=255) gCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTcccc < 2:722958/48‑1 (MQ=255) gCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTcccc > 1:722958/1‑48 (MQ=255) gCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAGGAAGt < 1:878126/150‑1 (MQ=255) | GCGAACGATAGTCGCCTTCTGCAGATGATGTAAAGCTTTGCCCAGGTGGCGGAATTGGTAGACGCGCTGGTTTCAGGTATCAGTGACCGCAAGGTCGTGGAAGTTCGAGTCTTCTCCTGGGCACCATACAAAAACCCACTAGCTTGCTAGTGGGTTTTTTCTTGCCTGCGATTTATGTGCCCCCTCCCCCCCTTGCACCATGGTCTGACCATGAATTTCTCGTCACACGGCCACTTGAGAAACAATTTCGTTTACCATTGTTTCCAAATATGTAGCCGTAAGCGAGGAAGT > NC_002947/5549510‑5549800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |