breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsPputida_HGL_35_S359_L002_R1_001.good.fq1,648,479216,216,720100.0%131.2 bases150 bases94.6%
errorsPputida_HGL_35_S359_L002_R2_001.good.fq1,648,479216,216,720100.0%131.2 bases150 bases93.0%
total3,296,958432,433,440100.0%131.2 bases150 bases93.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0029476,181,92965.42.4100.0%Pseudomonas putida KT2440 chromosome, complete genome.
total6,181,929100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000062736
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000787
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.039

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0029470.79569

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input19:45:50 26 Mar 202019:46:42 26 Mar 202052 seconds
Read alignment to reference genome19:46:42 26 Mar 202019:54:36 26 Mar 20207 minutes 54 seconds
Preprocessing alignments for candidate junction identification19:54:36 26 Mar 202019:55:20 26 Mar 202044 seconds
Preliminary analysis of coverage distribution19:55:20 26 Mar 202019:57:06 26 Mar 20201 minute 46 seconds
Identifying junction candidates19:57:06 26 Mar 202020:06:23 26 Mar 20209 minutes 17 seconds
Re-alignment to junction candidates20:06:24 26 Mar 202020:08:22 26 Mar 20201 minute 58 seconds
Resolving best read alignments20:08:22 26 Mar 202020:09:48 26 Mar 20201 minute 26 seconds
Creating BAM files20:09:48 26 Mar 202020:11:23 26 Mar 20201 minute 35 seconds
Tabulating error counts20:11:23 26 Mar 202020:11:57 26 Mar 202034 seconds
Re-calibrating base error rates20:11:57 26 Mar 202020:11:59 26 Mar 20202 seconds
Examining read alignment evidence20:11:59 26 Mar 202020:31:44 26 Mar 202019 minutes 45 seconds
Polymorphism statistics20:31:44 26 Mar 202020:31:46 26 Mar 20202 seconds
Output20:31:46 26 Mar 202020:34:22 26 Mar 20202 minutes 36 seconds
Total 48 minutes 31 seconds