Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,741,2770CT70.7% 53.8 / 23.1 41intergenic (+468/+284)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (4/8);  new base T (15/14);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTC‑C‑GCCAAC‑T‑GCATTCGTGAA‑CCAAGC‑AACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACC  >  NC_002947/4741155‑4741405
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gTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑CTGCGACT‑T‑GCATTCGTGaatccag                                                                                                          >  1:811231/1‑145 (MQ=255)
          cATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGccc                                                                                              <  1:918568/150‑1 (MQ=255)
                       gATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGtcctc                                                                                 >  2:93493/1‑150 (MQ=255)
                         tGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑C‑TGCGACTT‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTcctcct                                                                               >  1:68148/1‑150 (MQ=255)
                          ggTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGATCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTCGT‑GGCTGG‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTg                                                                              <  1:1023288/150‑1 (MQ=255)
                            tCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCa                                                                            <  1:1393985/150‑1 (MQ=255)
                                   gtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTc                                                                     <  2:68148/150‑1 (MQ=255)
                                           cgtaaTCACGCGGCGTCACCGGCCATGGCGTATTGAAATGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑CTGCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTgg                                                             <  1:542648/147‑1 (MQ=255)
                                                    gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGATCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑CCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCCCCTCGACCTTTGCCAgcggcc                                                    >  2:477507/1‑149 (MQ=255)
                                                    gccgcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggcg                                                    >  1:1498669/4‑150 (MQ=255)
                                                          tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCtt                                                               >  1:1331616/1‑133 (MQ=255)
                                                          tCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCtt                                                               <  2:1331616/133‑1 (MQ=255)
                                                           cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                             <  1:1490661/150‑1 (MQ=255)
                                                           cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                             <  2:811231/150‑1 (MQ=255)
                                                               ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTc                                                                     <  1:861880/122‑1 (MQ=255)
                                                               ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTc                                                                     >  2:861880/1‑122 (MQ=255)
                                                                   aTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCa                                                          <  2:85065/129‑1 (MQ=255)
                                                                   aTGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCa                                                          >  1:85065/1‑129 (MQ=255)
                                                                           ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑C‑TGCGACTT‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                             <  2:1473424/150‑1 (MQ=255)
                                                                              aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                          >  1:1174807/1‑150 (MQ=255)
                                                                                cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑C‑TGCGAC‑TAGCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                        <  2:1168611/150‑1 (MQ=255)
                                                                                 tGGGTATAGGTCCAGCTCTGTTCACCTTCGTGCTTGAG‑CA‑GCTT‑C‑TGCGACTT‑GCATTCGTGAA‑ACTCGC‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGTGCCTTGATCACGGTCACACCGCGATAGGcc                       <  1:93493/150‑1 (MQ=255)
                                                                                 tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                       >  1:973100/1‑150 (MQ=255)
                                                                                 tGGGTATAGGTCCACCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑C‑TGCGAG‑T‑TGATCCCGGGA‑TTTGGCGTTCAGGGCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                       <  1:1089932/150‑1 (MQ=255)
                                                                                   ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                     <  1:477507/150‑1 (MQ=255)
                                                                                        aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACt                >  1:1329559/1‑150 (MQ=255)
                                                                                        aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACt                <  2:1498669/150‑1 (MQ=255)
                                                                                           tCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑CTGCGACT‑T‑GCATTCGTGaat                                                                                                              <  1:1297462/55‑2 (MQ=255)
                                                                                              cgCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAcc          >  1:804859/2‑150 (MQ=255)
                                                                                              aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                              >  2:538488/1‑114 (MQ=255)
                                                                                              aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                              <  1:538488/114‑1 (MQ=255)
                                                                                              aGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑C‑TGCGACTT‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAcc          >  2:685855/1‑150 (MQ=255)
                                                                                                ctctGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCg        >  2:1061482/1‑150 (MQ=255)
                                                                                                 tctGGTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAcaagc       >  2:1287567/1‑146 (MQ=255)
                                                                                                 tctGGTCACCTTCGTGCTTGAG‑CA‑GCTT‑C‑TGCGACTT‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCgg       >  2:1193226/1‑150 (MQ=255)
                                                                                                    ggTCACCTTCGTGTTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa    <  1:685855/150‑1 (MQ=255)
                                                                                                     gTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc   >  1:1013246/1‑150 (MQ=255)
                                                                                                     gTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                              >  2:1320424/1‑107 (MQ=255)
                                                                                                     gTCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                              <  1:1320424/107‑1 (MQ=255)
                                                                                                      tCACCTTCGTGCTTGAGCAG‑CTTC‑T‑GCGACT‑T‑GCATTCGTGAATCCAGGC‑‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAcc  <  2:973100/150‑1 (MQ=255)
                                                                                                           ttCGTGCTTGAG‑CA‑GCTT‑C‑TGCGACTT‑GCATTCGTGAA‑TCCAGG‑CACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGAc                                                                  <  2:1111401/81‑1 (MQ=255)
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GTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTC‑C‑GCCAAC‑T‑GCATTCGTGAA‑CCAAGC‑AACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACC  >  NC_002947/4741155‑4741405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑