BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,994,811	Δ1 bp	5.7%	coding (1482/1599 nt)	PP_5251 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,994,811	0	C	.	5.7%	336.7 / 14.6	105	coding (1482/1599 nt)	PP_5251	transporter
Reads supporting (aligned to +/- strand): ref base C (50/49); new base . (3/3); total (53/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 111 total aligned reads displayed.

CTTCCAGCAGGCGGCGCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAAC  >  NC_002947/5994664‑5994960

      gcagGCGGCGCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGAc                                                                                                                                                >  2:1879337/1‑150 (MQ=255)

       cagGCGGCGCGGGAGCTGGAAGCGATGCGCAAGCGCAAGGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGAcc                                                                                                                                               >  2:575283/1‑150 (MQ=255)

         ggcggcGCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCC‑gg                                                                                                                                           >  2:1307807/1‑149 (MQ=255)

           cggcgCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCCTCTGCGACATCACCACCGGGTGACCC‑gtg                                                                                                                                          <  1:1817238/150‑1 (MQ=255)

              cgcgGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCCGgtggtg                                                                                                                                       <  1:242738/150‑1 (MQ=255)

               gcgGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGa                                                                                                                                      >  1:54759/1‑150 (MQ=255)

                cgGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGaa                                                                                                                                     <  1:843507/150‑1 (MQ=255)

                cgGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCCGGTGGTGat                                                                                                                                     >  2:1001889/1‑149 (MQ=255)

                 gggAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCCGGTGGTGatg                                                                                                                                    <  1:1001889/150‑3 (MQ=255)

                  ggAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACt                                                                                                                                   >  2:1836442/1‑150 (MQ=255)

                   gAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTg                                                                                                                                  >  2:1430029/1‑150 (MQ=255)

                    ggCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGCGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGa                                                                                                                                 <  2:863485/149‑1 (MQ=255)

                     gCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCCGGTGGTGatgtcgc                                                                                                                                <  1:1307807/150‑7 (MQ=255)

                       tGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAAc                                                                                                                                    >  2:685413/1‑144 (MQ=255)

                       tGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAAc                                                                                                                                    <  1:685413/144‑1 (MQ=255)

                       tGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAAc                                                                                                                                    <  1:685549/144‑1 (MQ=255)

                       tGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAAc                                                                                                                                    >  2:685549/1‑144 (MQ=255)

                        ggAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGAt                                                                                                                             >  1:554415/1‑150 (MQ=255)

                        ggAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGAt                                                                                                                             >  2:322518/1‑150 (MQ=255)

                         gAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATc                                                                                                                            <  1:1817937/150‑1 (MQ=255)

                         gAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATc                                                                                                                            <  2:829151/150‑1 (MQ=255)

                         gAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GAGGTGAACTGAAGATc                                                                                                                            >  2:1626278/1‑150 (MQ=255)

                         gAAGCGATGCGCAAGCGCAATGGCGCATTGTCGGGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATc                                                                                                                            >  1:1273667/1‑150 (MQ=255)

                               aTGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGgcg                                                                                                                      >  1:1629086/1‑150 (MQ=255)

                                tGCGCAAGCGCAATGGCGCATTGGCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGgcgc                                                                                                                     >  2:574638/1‑150 (MQ=255)

                                  cgcaagcgcaaTGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCgg                                                                                                                   <  1:967953/150‑1 (MQ=255)

                                   gcaagcgcaaTGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCggg                                                                                                                  >  1:1021950/1‑150 (MQ=255)

                                     aagcgcaaTGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTc                                                                                                                >  2:2026939/1‑150 (MQ=255)

                                         gcaaTGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGa                                                                                                            >  1:1441551/1‑150 (MQ=255)

                                            atcgcggATTGCCGAGAGGTTTGATACGGCACAGTCCCTTTCCCTAGTCGTGGCTTTTGTTCTGCTTCGATGGGTCGTGGCGGGAGGGTCTGCGACATCACCACCGGGTGACCG‑GGGGTGAACTGAAGATCGAGGCGCGGGTCAGGAtgc                                                                                                         <  2:389133/147‑1 (MQ=255)

                                              ggCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGAtgctg                                                                                                       <  2:1710406/150‑1 (MQ=255)

                                                   gatctCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGTAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGTTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGAc                                                                                                  <  1:294215/146‑1 (MQ=255)

                                                         gTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATgg                                                                                            >  2:445331/1‑150 (MQ=255)

                                                              ggTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTg                                                                                       >  2:1830575/1‑150 (MQ=255)

                                                               gTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGa                                                                                      <  1:1463697/150‑1 (MQ=255)

                                                                ttGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAg                                                                                     <  1:376543/150‑1 (MQ=255)

                                                                  gATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCAAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACAGAGGGGGTTGAGGt                                                                                   >  2:2287565/1‑150 (MQ=255)

                                                                   aTGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTg                                                                                  >  2:1474455/1‑150 (MQ=255)

                                                                    tGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAAc                                                                                                                                    >  1:2048025/1‑99 (MQ=255)

                                                                    tGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAAc                                                                                                                                    <  2:2048025/99‑1 (MQ=255)

                                                                        ggACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGAt                                                                             >  1:1915782/1‑150 (MQ=255)

                                                                        ggACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGAt                                                                             >  1:363434/1‑150 (MQ=255)

                                                                        ggACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGAt                                                                             >  1:1196639/1‑150 (MQ=255)

                                                                          aCAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTc                                                                           <  1:1836442/150‑1 (MQ=255)

                                                                          aCAGTGCTGATCCGACGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTCGCTTTTACAGCAGCAACAGTGTTACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTc                                                                           <  1:2026939/150‑1 (MQ=255)

                                                                             gTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGCGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGc                                                                        <  1:641167/150‑1 (MQ=255)

                                                                               gCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGATCGATGGGGTTGAGGTGCGGATTCGGCAt                                                                      >  1:1717577/1‑150 (MQ=255)

                                                                               gCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCAt                                                                      <  1:2296010/150‑1 (MQ=255)

                                                                                cTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATc                                                                     >  1:1704788/1‑150 (MQ=255)

                                                                                cTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATc                                                                     >  2:1320777/1‑150 (MQ=255)

                                                                                cTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATc                                                                     <  2:638172/150‑1 (MQ=255)

                                                                                cTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATc                                                                     >  2:1100062/1‑150 (MQ=255)

                                                                                   aTCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTAt                                                                  <  1:1320777/150‑1 (MQ=255)

                                                                                    tCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTAtt                                                                 <  1:1879337/150‑1 (MQ=255)

                                                                                     ccGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTg                                                                <  1:1290859/150‑1 (MQ=255)

                                                                                      cGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTgg                                                               >  1:1721146/1‑150 (MQ=255)

                                                                                       ggCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGc                                                              <  1:322518/150‑1 (MQ=255)

                                                                                       ggCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGc                                                              <  2:1991174/150‑1 (MQ=255)

                                                                                        gCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑gtg                                                                                                                                          >  2:71947/1‑73 (MQ=255)

                                                                                        gCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑gtg                                                                                                                                          <  1:71947/73‑1 (MQ=255)

                                                                                         cGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCtg                                                            <  2:1196639/150‑1 (MQ=255)

                                                                                              tgGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTggg                                                       <  1:559429/150‑1 (MQ=255)

                                                                                              tgGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTggg                                                       <  2:1795262/150‑1 (MQ=255)

                                                                                                gCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGa                                                     >  2:2173940/1‑150 (MQ=255)

                                                                                                    gAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCa                                                                                                     <  2:1269650/98‑1 (MQ=255)

                                                                                                    gAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCa                                                                                                     >  1:1269650/1‑98 (MQ=255)

                                                                                                    gAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCAt                                                 <  1:445331/150‑1 (MQ=255)

                                                                                                     aGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATa                                                <  1:1474455/150‑1 (MQ=255)

                                                                                                        tcttcttcGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAgg                                             <  1:1626278/150‑1 (MQ=255)

                                                                                                          ttcttcGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGca                                           <  2:54759/150‑1 (MQ=255)

                                                                                                            cttcGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCa                                                                                                               >  2:1646134/1‑80 (MQ=255)

                                                                                                            cttcGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCa                                                                                                               <  1:1646134/80‑1 (MQ=255)

                                                                                                            cttcGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCgg                                                                         >  1:317914/1‑118 (MQ=255)

                                                                                                            cttcGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCgg                                                                         <  2:317914/118‑1 (MQ=255)

                                                                                                             ttcGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCacac                                        <  1:1430029/150‑1 (MQ=255)

                                                                                                                  ggAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATaa                                               >  1:1582159/1‑138 (MQ=255)

                                                                                                                  ggAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATaa                                               <  2:1582159/138‑1 (MQ=255)

                                                                                                                    aaTCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCAt                                                                      <  2:1943007/113‑1 (MQ=255)

                                                                                                                    aaTCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGAt                                 <  2:1721146/150‑1 (MQ=255)

                                                                                                                    aaTCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGAt                                 <  2:2170144/150‑1 (MQ=255)

                                                                                                                         tGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAgg                                             >  2:259119/1‑133 (MQ=255)

                                                                                                                         tGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAgg                                             <  1:259119/133‑1 (MQ=255)

                                                                                                                            cTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCgg                                                                         >  2:1794004/1‑102 (MQ=255)

                                                                                                                            cTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCgg                                                                         <  1:1794004/102‑1 (MQ=255)

                                                                                                                              ggAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGACCGCGGCGAGGATCAGCCTGCTGCAGTCCGAGCTGGTTTAGGGTGTGTTTGTGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCtt                       >  2:2017232/1‑150 (MQ=255)

                                                                                                                               gAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCTCGGGTCAGGATGCTGCAGACCTATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTa                      <  2:554415/150‑1 (MQ=255)

                                                                                                                               gAGCATCTGCGACATAACCACCAGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTa                      >  2:207915/1‑150 (MQ=255)

                                                                                                                                    tCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGa                                                                                      <  2:1690530/81‑1 (MQ=255)

                                                                                                                                    tCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTAATGCCCGCCCTTACggac                 >  2:943752/1‑148 (MQ=255)

                                                                                                                                      tGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCa               >  2:763682/1‑150 (MQ=255)

                                                                                                                                        cGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCAtt             >  1:708682/1‑150 (MQ=255)

                                                                                                                                         gACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTc            >  1:1198775/1‑150 (MQ=255)

                                                                                                                                         gACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTc            >  1:1083325/1‑150 (MQ=255)

                                                                                                                                           gttCACCA‑CGGGTCACCCGGTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTcgc          >  2:1357878/3‑150 (MQ=255)

                                                                                                                                            aTCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTcgcg         >  1:1997819/1‑150 (MQ=255)

                                                                                                                                              caccacCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCggg       >  1:2264962/1‑150 (MQ=255)

                                                                                                                                              caccacCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCggg       >  1:1598989/1‑150 (MQ=255)

                                                                                                                                                 caccGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGa    >  2:809563/1‑150 (MQ=255)

                                                                                                                                                   ccGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAAc  <  1:207915/150‑1 (MQ=255)

                                                                                                                                                   ccGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTAAGGCAGCCGTTTCCTGATGTTGTTGAGGTGCGGCTTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAAc  <  1:2273311/150‑1 (MQ=255)

CTTCCAGCAGGCGGCGCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAAC  >  NC_002947/5994664‑5994960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`