Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,143,680:1 +T 5.4% coding (417/435 nt) PP_1899 → ExbD/TolR family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,143,6801.T 5.4% 379.2 / 14.3 93coding (417/435 nt)PP_1899ExbD/TolR family protein
Reads supporting (aligned to +/- strand):  ref base . (44/44);  new base T (2/3);  total (46/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

GAGGCCATAGAGCGCGAGTCGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTAAGGGGT  >  NC_002947/2143531‑2143823
                                                                                                                                                      |                                                                                                                                                
gAGGCCATAGAGCGCGAGTCGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAggcg                                                                                                                                                   <  2:1520167/150‑1 (MQ=255)
       tAGAGCGCGAGTCGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAgg                                                                                                                                          >  1:458060/1‑150 (MQ=255)
           gcgcGAGTCGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCTGCCCCGGTTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCGTGCC‑CTGGGcc                                                                                                                                       <  1:735935/150‑2 (MQ=255)
             gcgAGTCGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCATCAAGGCGTGCC‑CTGGGccgc                                                                                                                                     >  1:662470/1‑147 (MQ=255)
                  tCGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGa                                                                                                                               <  2:318731/150‑1 (MQ=255)
                   cGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGAt                                                                                                                              <  1:2182349/150‑1 (MQ=255)
                    gggCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATg                                                                                                                             <  1:1616682/150‑1 (MQ=255)
                    gggCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATg                                                                                                                             >  1:1617263/1‑150 (MQ=255)
                    gggCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCATCAAGGCGTGCC‑CTGGGC‑CGCCtcgacg                                                                                                                             <  2:662470/150‑6 (MQ=255)
                     ggcggcGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATgg                                                                                                                            >  2:1864960/1‑150 (MQ=255)
                      gcggcgACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGc                                                                                                                           >  1:1349921/1‑150 (MQ=255)
                         gcgACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCttt                                                                                                                        <  1:391586/150‑1 (MQ=255)
                         gcgACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCttt                                                                                                                        <  2:1056024/150‑1 (MQ=255)
                            acaacaAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGc                                                                                                                     <  2:577330/150‑1 (MQ=255)
                            acaacaAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGc                                                                                                                     <  2:153753/150‑1 (MQ=255)
                             caacaaGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGcc                                                                                                                    <  2:596415/150‑1 (MQ=255)
                              aacaaGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCtt                                                                                                                                 <  2:983590/136‑1 (MQ=255)
                              aacaaGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCtt                                                                                                                                 >  1:983590/1‑136 (MQ=255)
                                 aaGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATc                                                                                                                >  2:1901197/1‑150 (MQ=255)
                                   gTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGc                                                                                                              >  2:134093/1‑150 (MQ=255)
                                    ttGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGTG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCt                                                                                                             >  2:419259/1‑150 (MQ=255)
                                     tGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCtt                                                                                                            <  1:1129444/150‑1 (MQ=255)
                                      gCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTg                                                                                                           <  1:1864960/150‑1 (MQ=255)
                                         gCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTc                                                                                                        >  1:1970077/1‑150 (MQ=255)
                                              ccATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTGAGCCAGATGTGCGT‑ATCAGCGCTGACTCG‑GCCTCACGGCGCGCCTTGATGGCTTTCGCCGCTCGCTTGCTCGCGGc                                                                                                   >  1:274124/1‑150 (MQ=255)
                                              ccATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGc                                                                                                   >  1:1051320/1‑150 (MQ=255)
                                               cATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGcc                                                                                                  >  2:830658/1‑150 (MQ=255)
                                                aTCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCt                                                                                                 <  2:1617263/150‑1 (MQ=255)
                                                 tCAGCGCCGACGGCAAGACCCCGCATCCGGCTGTGTGCACCGGAAGGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTg                                                                                                <  1:521024/150‑1 (MQ=255)
                                                    gcgcCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTa                                                                                             <  1:1901197/150‑1 (MQ=255)
                                                           cGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCggg                                                                                      <  1:1628866/150‑1 (MQ=255)
                                                            ggCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGc                                                                                     >  2:1118398/1‑150 (MQ=255)
                                                              cAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCAt                                                                                   >  2:1563445/1‑150 (MQ=255)
                                                               aaGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATc                                                                                  >  2:1400611/1‑150 (MQ=255)
                                                                  aCCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATg                                                                                                                             >  1:1529850/1‑104 (MQ=255)
                                                                  aCCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATg                                                                                                                             <  2:1529850/104‑1 (MQ=255)
                                                                     ccGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCggcgc                                                                            >  2:398084/1‑150 (MQ=255)
                                                                      cGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCggcgct                                                                           <  1:1047564/150‑1 (MQ=255)
                                                                      cGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCggcgct                                                                           >  2:2233354/1‑150 (MQ=255)
                                                                            aGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCggcgctggcgct                                                                     >  2:2155701/1‑150 (MQ=255)
                                                                                 gtgGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCtgctgc                                                                >  2:1767912/1‑150 (MQ=255)
                                                                                    gTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTc                                                             <  2:342178/150‑1 (MQ=255)
                                                                                     tCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCGTGCC‑CTGGGc                                                                                                                                        >  1:2191630/1‑75 (MQ=255)
                                                                                     tCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCGTGCC‑CTGGGc                                                                                                                                        <  2:2191630/75‑1 (MQ=255)
                                                                                     tCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTcc                                                            <  1:1956379/150‑1 (MQ=255)
                                                                                     tCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATAAATACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTcc                                                            >  1:1333688/1‑150 (MQ=255)
                                                                                       acagCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGGCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGc                                                          >  1:1760707/4‑150 (MQ=255)
                                                                                           cAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGTG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGa                                                      <  1:419259/150‑1 (MQ=255)
                                                                                           cAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTg                                                                                                >  2:1382843/1‑108 (MQ=255)
                                                                                           cAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTg                                                                                                <  1:1382843/108‑1 (MQ=255)
                                                                                            aaTGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAg                                                     <  1:398084/150‑1 (MQ=255)
                                                                                              tGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGc                                                   <  1:541198/150‑1 (MQ=255)
                                                                                                gATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCt                                                 <  2:782665/150‑1 (MQ=255)
                                                                                                 aTGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCtt                                                >  1:1217834/1‑150 (MQ=255)
                                                                                                 aTGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCtt                                                <  1:134093/150‑1 (MQ=255)
                                                                                                 aTGCTGCCGGCAAGCTCGGTGTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGCCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCtt                                                <  2:130152/150‑1 (MQ=255)
                                                                                                     tgcCGACAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTAt                                            <  1:1433437/150‑1 (MQ=255)
                                                                                                          gCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATcgccg                                       >  2:625622/1‑150 (MQ=255)
                                                                                                            aaGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCcgcg                                     >  1:18879/1‑150 (MQ=255)
                                                                                                             aGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGt                                    <  1:1790995/150‑1 (MQ=255)
                                                                                                              gCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTg                                   <  2:1970077/150‑1 (MQ=255)
                                                                                                               cTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCAAGCCTTGCTGGCTTTCGCCGATCGCGTGCTCGCTGCCTTGGATGCCGGGCTTCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTgg                                  >  2:851813/1‑150 (MQ=255)
                                                                                                                tCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGt                                 >  1:761023/1‑150 (MQ=255)
                                                                                                                tCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGt                                 >  1:1135247/1‑150 (MQ=255)
                                                                                                                 cGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTc                                <  1:2110940/150‑1 (MQ=255)
                                                                                                                 cGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGAGCATAAAATGCTTGTGGTCCTTCCTCCTCTGGAGGCCTTGTATCGCCGCGTGGTc                                <  1:407208/150‑1 (MQ=255)
                                                                                                                     ttCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCAcgc                            <  1:128129/150‑1 (MQ=255)
                                                                                                                        aGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCggcgctggcgct                                                                     >  2:1000527/1‑106 (MQ=255)
                                                                                                                        aGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCggcgctggcgct                                                                     <  1:1000527/106‑1 (MQ=255)
                                                                                                                         gCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCaa                        >  1:214076/1‑150 (MQ=255)
                                                                                                                         gCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTAGATCGGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCaa                        >  1:1874040/1‑150 (MQ=255)
                                                                                                                         gCCACTTGCGCAGGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCaa                        <  2:1333688/150‑1 (MQ=255)
                                                                                                                          ccAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAg                       >  2:1030606/1‑150 (MQ=255)
                                                                                                                           cAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGc                      <  1:1544441/150‑1 (MQ=255)
                                                                                                                            aGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCg                                                                                                       >  1:2124680/1‑68 (MQ=255)
                                                                                                                            aGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCg                                                                                                       <  2:2124680/68‑1 (MQ=255)
                                                                                                                             gTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCgg                    <  2:1473088/150‑1 (MQ=255)
                                                                                                                                 cgcATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGAGCAAGCGGGCAc                >  2:2246574/1‑150 (MQ=255)
                                                                                                                                  gcATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACg               >  2:419504/1‑150 (MQ=255)
                                                                                                                                  gcATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACg               >  2:1424382/1‑150 (MQ=255)
                                                                                                                                   cATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGt              >  1:118468/1‑150 (MQ=255)
                                                                                                                                    aTGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGa                                                      <  1:2003199/109‑1 (MQ=255)
                                                                                                                                    aTGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGa                                                      >  2:2003199/1‑109 (MQ=255)
                                                                                                                                        ccaccGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCt         <  2:1020022/150‑1 (MQ=255)
                                                                                                                                         caccGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTa        >  2:561830/1‑150 (MQ=255)
                                                                                                                                           ccGTCGAGGCG‑GCC‑CAGGGCACGGCTGGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTAAg      <  2:135071/150‑1 (MQ=255)
                                                                                                                                             atCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTAAggg    >  1:1864665/2‑150 (MQ=255)
                                                                                                                                              tCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGt                                    <  2:120578/117‑1 (MQ=255)
                                                                                                                                              tCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGt                                    >  1:120578/1‑117 (MQ=255)
                                                                                                                                              tCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCg                     <  2:2095002/132‑1 (MQ=255)
                                                                                                                                              tCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCg                     >  1:2095002/1‑132 (MQ=255)
                                                                                                                                              tCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTAAgggg   <  1:830658/150‑1 (MQ=255)
                                                                                                                                              tCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGACGGTGCGGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTAAgggg   >  1:1352074/1‑150 (MQ=255)
                                                                                                                                               cGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTAAGGGGt  <  1:1030606/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GAGGCCATAGAGCGCGAGTCGGGCGGCGACAACAAGTTGCCGCTGGCCATCAGCGCCGACGGCAAGACCCCGCATCAGGCTGTGGTCACCGCAATGGATGCTGCCGGCAAGCTCGGTTTCAGCCAGTTGCGCATGACCACCGTCGAGGCG‑GCC‑CAGGGCACGCCTTGATGGCTTTCGCCGATCGCTTGCTCGCTGCCTGGTATGCCGGGCATCCGGCGCTGGCGCTGCTGCGTCCGCTGGAGGCCTTGTATCGCCGCGTGGTCACGCGCAAGCGGGCACGTTTTCTAAGGGGT  >  NC_002947/2143531‑2143823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑