Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,688,234:1 +A 6.9% coding (308/1107 nt) PP_3253 → carboxylate‑amine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,688,2341.A 6.9% 410.5 / 23.7 101coding (308/1107 nt)PP_3253carboxylate‑amine ligase
Reads supporting (aligned to +/- strand):  ref base . (50/44);  new base A (2/5);  total (52/49)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

Only 100 of 114 total aligned reads displayed.

GCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCTGGTTACCG  >  NC_002947/3688085‑3688382
                                                                                                                                                        |                                                                                                                                                    
gCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc                                                                                                                                                       <  1:831349/150‑1 (MQ=255)
 cTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc‑c                                                                                                                                                     >  2:2034998/1‑150 (MQ=255)
         gCCGGTGTTCACCAACCTGTATGAAGCAAGTGAGTTTCTCCAGCGCAACCGTCAGCGGATGCCCGTGGCGTTCGTGACCTGGATAGCAAGGG‑‑CAAGGGCGGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGcaca                                                                                                                                             >  1:2280901/1‑150 (MQ=255)
          ccGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGcgcag                                                                                                                                            <  1:47020/150‑2 (MQ=38)
           cGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGcgcagg                                                                                                                                           <  2:423209/150‑3 (MQ=38)
             gtgtTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCACCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAggg                                                                                                                                         <  2:1752566/150‑1 (MQ=255)
                ttCACCAACCTGGATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCAc                                                                                                                                      >  2:1399895/1‑150 (MQ=255)
                 tCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCTTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGCGCAggggtggc                                                                                                                                     <  1:1989127/150‑5 (MQ=37)
                  cACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACta                                                                                                                                    >  1:1156385/1‑150 (MQ=255)
                   aCCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACtat                                                                                                                                   >  2:1996253/1‑150 (MQ=255)
                    ccAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACtata                                                                                                                                  >  2:1934906/1‑150 (MQ=255)
                      aaCCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGTGCACTATAag                                                                                                                                <  1:510576/150‑1 (MQ=255)
                      aaCCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAag                                                                                                                                <  2:2154344/150‑1 (MQ=255)
                       aCCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAagc                                                                                                                               >  2:988956/1‑150 (MQ=255)
                        ccTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAagca                                                                                                                              >  2:1952886/1‑150 (MQ=255)
                           gTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCt                                                                                                                           >  1:1984977/1‑150 (MQ=255)
                           gTATGAAGCACGTGACTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCt                                                                                                                           <  2:1622853/150‑1 (MQ=255)
                              tGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGtt                                                                                                                        >  1:508089/1‑150 (MQ=255)
                              tGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCATGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGtt                                                                                                                        >  1:581037/1‑150 (MQ=255)
                                  gCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                                    <  1:753953/150‑1 (MQ=255)
                                    aCGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgacga                                                                                                                  >  2:2010084/1‑150 (MQ=255)
                                     cGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCgacgac                                                                                                                 >  2:1010794/1‑150 (MQ=255)
                                      gTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACt                                                                                                                >  1:2159235/1‑150 (MQ=255)
                                      gTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACt                                                                                                                >  2:1126495/1‑150 (MQ=255)
                                          gTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCg                                                                                                            >  1:77886/1‑150 (MQ=255)
                                               tcCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCAtg                                                                                                       >  2:938882/1‑150 (MQ=255)
                                                 cAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑cg                                                                                                                                                    >  2:1981819/1‑103 (MQ=255)
                                                  aGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGAGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTgg                                                                                                    >  1:1705491/1‑150 (MQ=255)
                                                   gcgcAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGcc                                                                                                          <  2:1015541/143‑1 (MQ=255)
                                                   gcgcAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGcc                                                                                                          >  1:1015541/1‑143 (MQ=255)
                                                      ccaCCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGACGCGTGGCTGCTGCAGAAGCC‑CCCGGCACAAGGGCACTATACGCTGCTGTTCGACGACTACCCCCATGTGACAAg                                                                                                <  2:689744/148‑1 (MQ=255)
                                                      cAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAg                                                                                                <  2:1006251/150‑1 (MQ=255)
                                                         ccGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACAACTGCGCCGCTGGGTTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCg                                                                                             <  1:2034998/150‑1 (MQ=255)
                                                          cGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                                 <  2:1150516/97‑1 (MQ=25)
                                                          cGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                                 >  1:1150516/1‑97 (MQ=25)
                                                           gTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTa                                                                                           <  1:1126495/150‑1 (MQ=255)
                                                             cAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGc                                                                                         <  1:1952886/150‑1 (MQ=255)
                                                             cAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGc                                                                                         <  1:1996253/150‑1 (MQ=255)
                                                              aGCGGCTGCGCGTGGCATT‑G‑GCCGAAAAGGGGAGGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGcc                                                                                        <  2:1705491/150‑1 (MQ=255)
                                                                  gCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCt                                                                                    <  1:1010794/150‑1 (MQ=255)
                                                                      cgcgTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTTGCAAGGCGTAGCCTGCTCAAt                                                                                <  1:1449502/150‑1 (MQ=255)
                                                                        cgTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCt                                                                                       >  2:607371/1‑141 (MQ=255)
                                                                         gTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGc                                                                             <  2:2159235/150‑1 (MQ=255)
                                                                             cATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGc                                                                         <  2:1698541/150‑1 (MQ=255)
                                                                              aTT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc‑c                                                                                                                                                     >  1:1984592/1‑73 (MQ=255)
                                                                              aTT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc‑c                                                                                                                                                     <  2:1984592/73‑1 (MQ=255)
                                                                                t‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCAtg                                                                      <  1:854556/150‑1 (MQ=255)
                                                                                  g‑gCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGAAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCAtgt                                                                     >  2:1484353/1‑150 (MQ=255)
                                                                                       gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                                 >  1:109630/1‑70 (MQ=21)
                                                                                       gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                                 <  2:109630/70‑1 (MQ=21)
                                                                                       gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCACTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCAc                                                                 <  1:611346/150‑1 (MQ=255)
                                                                                           agGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTg                                                             <  1:1484353/150‑1 (MQ=255)
                                                                                            ggggATGGGGCCATACGCTGCGGCAAGCCACACCTGCGCCGCGTGGCTGCTGAAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTGAATGGCCTGCATGTGCACGTTgg                                                            >  1:974825/1‑150 (MQ=255)
                                                                                             gggATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGc                                                           <  2:1984977/150‑1 (MQ=255)
                                                                                             gggATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGc                                                           <  2:400894/150‑1 (MQ=255)
                                                                                               gATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTCGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGt                                                         >  2:893861/1‑150 (MQ=255)
                                                                                                  ggggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgcc                                                      >  1:1530646/1‑150 (MQ=255)
                                                                                                    ggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTCATCTTCTCAATGGCCTGCATGTGCACGTTGGCGTgccgc                                                    >  1:122738/1‑150 (MQ=255)
                                                                                                    ggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgc                                                    >  2:1263710/1‑150 (MQ=255)
                                                                                                     gCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAATGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgcc                                                   >  1:142386/1‑150 (MQ=255)
                                                                                                     gCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgcc                                                   >  1:1810435/1‑150 (MQ=255)
                                                                                                       cATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                                    <  2:703697/83‑1 (MQ=255)
                                                                                                       cATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                                    >  1:703697/1‑83 (MQ=255)
                                                                                                        aTACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                                    >  1:2147009/1‑82 (MQ=255)
                                                                                                        aTACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                                    <  2:2147009/82‑1 (MQ=255)
                                                                                                          aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCtt                                              <  1:1733877/150‑1 (MQ=255)
                                                                                                          aCGCTGCGGCAAGCCACCCCTGCGCCGCATGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCtt                                              <  2:581037/150‑1 (MQ=255)
                                                                                                            gCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCTACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                                            <  2:189505/150‑1 (MQ=255)
                                                                                                            gCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                                            <  1:877601/150‑1 (MQ=255)
                                                                                                             cTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgtg                                           >  1:2171137/1‑150 (MQ=255)
                                                                                                              tGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGa                                          >  1:2199779/1‑150 (MQ=255)
                                                                                                                cGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTCCCGCCATGTGGCAAGGCGTCGAGGGCGCAGTAGTCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGAcc                                        <  1:988956/150‑1 (MQ=255)
                                                                                                                  gCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGc                                      >  2:475389/1‑150 (MQ=255)
                                                                                                                     aGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATg                                   >  2:455662/1‑150 (MQ=255)
                                                                                                                     aGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATg                                   <  1:1934906/150‑1 (MQ=255)
                                                                                                                       ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCa                                 >  1:2101734/1‑150 (MQ=255)
                                                                                                                       ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCa                                 >  1:1863401/1‑150 (MQ=255)
                                                                                                                         aCCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAAc                               <  1:475389/150‑1 (MQ=255)
                                                                                                                          ccccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACt                              >  2:2065726/1‑150 (MQ=255)
                                                                                                                            ccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa                            <  1:1263710/150‑1 (MQ=255)
                                                                                                                            ccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGGAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa                            >  1:1313410/1‑150 (MQ=255)
                                                                                                                             cTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGAt                           >  2:568658/1‑150 (MQ=255)
                                                                                                                                 gccgcGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAAc                       >  1:907147/1‑150 (MQ=255)
                                                                                                                                     cgTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGcc                   <  2:86840/150‑1 (MQ=255)
                                                                                                                                           tgctgcAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGAcc                                        >  1:544064/1‑123 (MQ=255)
                                                                                                                                           tgctgcAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGAcc                                        <  2:544064/123‑1 (MQ=255)
                                                                                                                                               gcAGAAGCC‑CGCGGCAAAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCTg         <  1:1983824/150‑1 (MQ=255)
                                                                                                                                                 aGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCGTGCAACTGATCAACCGCCTTCTGCCCTg         >  2:1200588/1‑148 (MQ=255)
                                                                                                                                                 aGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCGTGCAACTGATCAACCGCCTTCTGCCCTg         <  1:1200588/148‑1 (MQ=255)
                                                                                                                                                 aGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCt          >  2:453279/1‑147 (MQ=255)
                                                                                                                                                 aGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCt          <  1:453279/147‑1 (MQ=255)
                                                                                                                                                  gAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCTGGtt      >  1:428175/1‑150 (MQ=255)
                                                                                                                                                   aaGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCAc                                                                 >  2:425085/1‑90 (MQ=255)
                                                                                                                                                   aaGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCAc                                                                 <  1:425085/90‑1 (MQ=255)
                                                                                                                                                      cc‑cGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATg                                                                               <  1:1259417/73‑1 (MQ=255)
                                                                                                                                                      cc‑cGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATg                                                                               >  2:1259417/1‑73 (MQ=255)
                                                                                                                                                      cc‑cGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCATGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCTGGTTACCg  >  1:848890/1‑150 (MQ=255)
                                                                                                                                                       c‑cGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                                            >  1:997869/1‑107 (MQ=255)
                                                                                                                                                       c‑cGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTGTGCCCt          >  2:1624818/1‑141 (MQ=255)
                                                                                                                                                       c‑cGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCt          <  1:1624818/141‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
GCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATT‑G‑GCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCTGCCCTGGTTACCG  >  NC_002947/3688085‑3688382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑