| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_002947 | 4340085 = | 38 (0.430) | 12 (0.190) | 8/180 | NT | 42.5% | intergenic (+26/‑305) | ribAB‑II/PP_3814 | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein |
| ? | NC_002947 | 4340169 = | 5 (0.080) | intergenic (+110/‑221) | ribAB‑II/PP_3814 | 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/polyamine ABC transportersubstrate‑binding protein | |||||
TCTGCAGGACCTTGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCG > NC_002947/4339936‑4340200 | gctaaaacaaagtgttctggtggccgtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaag > 2:816269‑M1/150‑150 (MQ=255) taaaacaaagtgttctggtggccgtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattgcaatattttggcaagaggtatggggcgaccggccatcacgccgccctgtcaaaagcaccaaggg < 1:114453‑M1/3‑1 (MQ=255) aaacaaagtgttctggtggccgtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGcc < 2:223408‑M1/5‑1 (MQ=255) aGGGCCTTGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGAGCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGc > 1:1309437/1‑148 (MQ=255) aGGGCCTTGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGc < 2:1309437/148‑1 (MQ=255) gACCTTGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTg > 2:1989364/1‑150 (MQ=255) aaagtgttctggtggccgtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactcagccctgtcaaaagcaccaaGGGCCCTGt < 1:2212239‑M1/9‑1 (MQ=255) aCCTTGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTTAGAACACACAAGCAATTGATTTAGCGGGGCCCTGt < 1:745499/150‑1 (MQ=255) ccTTGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTa > 1:1624433/1‑150 (MQ=255) ttGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAgg > 2:719705/1‑150 (MQ=255) ttctggtggccgtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGc > 1:1619302‑M1/136‑150 (MQ=255) gTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCgg < 1:1644707/150‑1 (MQ=255) ctgggggccgtttttaatcgtaggtgccggcctgcagactcaagggctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcacttcgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCgg > 1:134401‑M1/134‑150 (MQ=255) tGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCggg < 2:144677/150‑1 (MQ=255) tGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCggg < 1:2323203/150‑1 (MQ=255) aaaGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTa < 1:2203665/149‑1 (MQ=255) gCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAccc > 2:1655236/1‑150 (MQ=255) gCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAccc > 2:2228903/1‑150 (MQ=255) cgtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTAccc < 1:308262‑M1/25‑1 (MQ=255) agtttttgattgtaggtgttctgctgcagactcaatgtctacggtatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTAccc > 2:1084691‑M1/126‑150 (MQ=255) gtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGcc < 2:1150869‑M1/5‑1 (MQ=255) gtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGcc > 1:1150869‑M1/125‑129 (MQ=255) gtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCcg < 1:1084691‑M1/26‑1 (MQ=255) gtttttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactacgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCcg < 1:2222916‑M1/26‑1 (MQ=255) cTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCgg > 1:1427261/1‑141 (MQ=255) cTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCgg < 2:1427261/141‑1 (MQ=255) tGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCcgc < 2:1586178/150‑1 (MQ=255) tGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCcgc < 1:394902/150‑1 (MQ=255) ttaattgtaggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGaa > 1:46525‑M1/121‑150 (MQ=255) attgtgggtgccggcctgcagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGc > 1:1433477‑M1/118‑150 (MQ=255) cTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTCGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGa < 2:1351463/150‑1 (MQ=255) tGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAACCAACTGATGTAGCGGTGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAg < 1:407409/150‑1 (MQ=255) gggCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGttt > 2:1233017/1‑133 (MQ=255) gggCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGttt < 1:1233017/133‑1 (MQ=255) gggCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAggg < 1:1905765/150‑1 (MQ=255) ccgccgCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGATGGTGa < 1:1175921/150‑1 (MQ=255) gccgcTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGaca < 1:1655236/150‑1 (MQ=255) aaaTACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGa < 1:719705/150‑1 (MQ=255) cagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGt > 2:937547‑M1/100‑108 (MQ=255) cagactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGt < 1:937547‑M1/9‑1 (MQ=255) agactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCt < 2:27731‑M1/7‑1 (MQ=255) agactcaaggtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCt > 1:27731‑M1/99‑105 (MQ=255) tACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCgg < 1:1730189/114‑1 (MQ=255) tACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCgg > 2:1730189/1‑114 (MQ=255) ctctaggcctacgggatattattttatggtagtctaagattttcgaaagaggtatggggcgactggccatcactccgccatctcaaaagcaccaaGGGCCCTGTAGGAGCGGGGTTACCCGCGAATGCGAGGGTGACACCACCGACGCAt > 2:114453‑M1/96‑150 (MQ=255) gtctacgggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg > 1:2069145‑M1/90‑150 (MQ=255) gCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg < 1:1989364/150‑1 (MQ=255) cGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTaaa < 1:1805071/150‑1 (MQ=255) ggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg < 1:668461‑M1/61‑1 (MQ=255) ggatatcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg > 2:668461‑M1/83‑143 (MQ=255) atcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCgg > 1:300730‑M1/79‑95 (MQ=255) atcatattatggtattccaagattttggcaagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCgg < 2:300730‑M1/17‑1 (MQ=255) caagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg > 2:1125839‑M1/51‑111 (MQ=255) caagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg < 1:1125839‑M1/61‑1 (MQ=255) aagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg > 1:2284345‑M1/50‑110 (MQ=255) aagaggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg < 2:2284345‑M1/61‑1 (MQ=255) ggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg > 1:235762‑M1/46‑106 (MQ=255) ggtatggggcgaccggccatcactccgccctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg < 2:235762‑M1/61‑1 (MQ=255) aCCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGTCGGTGGTGTCACCCTCGCATTCGCgg > 2:2078429/1‑106 (MQ=17) cccGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGAcaccac < 2:625374/91‑1 (MQ=255) cccGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGAcaccac > 1:625374/1‑91 (MQ=255) cccGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg < 2:1428979/105‑1 (MQ=255) cccGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg > 1:1428979/1‑105 (MQ=255) cGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGt < 2:2306716/81‑1 (MQ=255) cGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGt > 1:2306716/1‑81 (MQ=255) gatggatgGCTGAGAACACACATGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAccc < 1:2222758/63‑1 (MQ=255) gatggatgGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTAccc > 2:2222758/1‑63 (MQ=255) gatggCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGTAGTGATGGCCg > 2:2222916/1‑150 (MQ=255) ctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAAc > 1:442152‑M1/17‑83 (MQ=255) ctgtcaaaagcaccaaGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAAc < 2:442152‑M1/67‑1 (MQ=255) aTTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcg < 1:846818/70‑1 (MQ=255) aTTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTcg > 2:846818/1‑70 (MQ=255) GGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg < 2:1004468‑M1/61‑1 (MQ=255) GGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCgg > 1:1004468‑M1/1‑61 (MQ=255) | TCTGCAGGACCTTGGCGTGGGCAAGCTGCGTCACCTGGGGCCGCCGCTGAAATACGCGGGGCTGGCGGGCTATGAGCTGGAGGTGGTGCAGAGCATCCCGTTCGACCCGGGGATGGATGGCTGAGAACACACAAGCAATTGATTTAGCGGGGCCCTGTAGGAGCGGGTTTACCCGCGAATGCGAGGGTGACACCACCGACGCATTCGCGGGTAAACCCGCTCCTACAGGGCCCTTGGTGCTTTTGACAGGGCGGAGTGATGGCCG > NC_002947/4339936‑4340200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |