BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,339	T→A	5.4%	Q15L (CAG→CTG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,339	0	T	A	5.4%	341.7 / 8.3	111	Q15L (CAG→CTG)	trmE	GTPase
Reads supporting (aligned to +/- strand): ref base T (45/59); new base A (3/3); total (49/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 111 total aligned reads displayed.

AATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGC  >  NC_002947/6191‑6479

aaTCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTg                                                                                                                                              <  2:1205863/150‑1 (MQ=255)

 aTCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTgg                                                                                                                                             <  2:2189428/150‑1 (MQ=255)

 aTCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTgg                                                                                                                                             >  1:186347/1‑150 (MQ=255)

    ccTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGcc                                                                                                                                          <  1:726880/150‑1 (MQ=255)

       tCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTg                                                                                                                                       <  2:725703/150‑1 (MQ=255)

        cGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTgg                                                                                                                                      <  2:531258/150‑1 (MQ=255)

           ccAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGa                                                                                                                                   <  2:1614005/150‑1 (MQ=255)

             aGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATg                                                                                                                                 <  1:2202222/150‑1 (MQ=255)

             aGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATg                                                                                                                                 >  2:831666/1‑150 (MQ=255)

               cACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggc                                                                                                                               <  1:829174/150‑1 (MQ=255)

               cACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCggtgtg                                                                                                                               <  1:2317965/150‑6 (MQ=255)

                aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGCCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcg                                                                                                                              >  1:1428694/1‑150 (MQ=255)

                    gCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCGGTTCGTTGCAGCCAGAGGGCCGGAAAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGa                                                                                                                          <  2:554134/150‑1 (MQ=255)

                       cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATgg                                                                                                                       >  1:619601/1‑150 (MQ=255)

                        ttCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACCACATGAAAATCACCCAAAAGCCGAGGCGCGGGGCGTTGGCGATGGCGGCGATGGt                                                                                                                      >  2:611817/1‑150 (MQ=255)

                          cATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGttt                                                                                                                    <  2:186347/150‑1 (MQ=255)

                           aTCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTc                                                                                                                   <  1:2312340/150‑1 (MQ=255)

                            tcgtcgCGGAATGGACCGTAATGGGCACGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCa                                                                                                                  <  1:486639/150‑1 (MQ=255)

                                  cgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGcaca                                                                                                            <  1:831666/150‑1 (MQ=255)

                                   ggAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCa                                                                                                                  >  2:374588/1‑143 (MQ=255)

                                   ggAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCa                                                                                                                  <  1:374588/143‑1 (MQ=255)

                                      aTGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAgtgt                                                                                                        <  2:1415611/150‑1 (MQ=255)

                                        ggACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc                                                                                                      <  2:18780/150‑1 (MQ=255)

                                            cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGc                                                                                                  >  2:1595091/1‑150 (MQ=255)

                                            cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAAAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCAc                                                                                                                 >  2:388013/1‑135 (MQ=255)

                                              tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                             >  2:404061/1‑121 (MQ=255)

                                              tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                             <  1:404061/121‑1 (MQ=255)

                                              tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGACTTACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                <  1:611817/150‑1 (MQ=255)

                                                 tGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCAGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGTCGTGGCGATGGCGGCGATGGTTTCACGc                                                                                                               <  1:1594031/132‑1 (MQ=255)

                                                 tGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCAGACAACCTGACAATTCCCACACCGCCCCGGCCCTGCGCCGTGGCGATGGCGGCGATGGTTTCACGc                                                                                                               >  2:1594031/1‑132 (MQ=255)

                                                  gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                            <  2:1407494/150‑1 (MQ=255)

                                                    gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACg                                                                                                                <  2:840495/128‑1 (MQ=255)

                                                    gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACg                                                                                                                >  1:840495/1‑128 (MQ=255)

                                                    gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGc                                                                                          <  1:1516912/150‑1 (MQ=255)

                                                    gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCggt                                                                                                                                  <  1:123350/110‑3 (MQ=255)

                                                    gCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCggt                                                                                                                                  >  2:123350/1‑108 (MQ=255)

                                                     cATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATgg                                                                                                                       <  2:749058/120‑1 (MQ=255)

                                                         ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTa                                                                                     <  1:504826/150‑1 (MQ=255)

                                                                     ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGt                                                                                                                      <  2:1406601/105‑1 (MQ=255)

                                                                     ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGt                                                                                                                      >  1:1406601/1‑105 (MQ=255)

                                                                     ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCATATAAAAAAAAAACGa                                                                         <  2:468347/150‑1 (MQ=255)

                                                                      gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                        <  1:516106/150‑1 (MQ=255)

                                                                         cGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                     <  1:2031589/150‑1 (MQ=255)

                                                                                     aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAg                                                                                                           <  1:1783250/100‑1 (MQ=255)

                                                                                     aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAg                                                                                                           >  2:1783250/1‑100 (MQ=255)

                                                                                     aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                         <  2:1638660/150‑1 (MQ=255)

                                                                                     aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                         <  1:1595091/150‑1 (MQ=255)

                                                                                      aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                        >  1:1788441/1‑150 (MQ=255)

                                                                                       cAGGCCGGCCTTGGCAGCCAGAGGGCCGGGAAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGccc                                                       >  1:1814483/1‑150 (MQ=255)

                                                                                          gccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCAc                                                    >  2:282320/1‑150 (MQ=255)

                                                                                            cggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTa                                                  >  1:278041/1‑150 (MQ=255)

                                                                                            cggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTa                                                  >  1:2320732/1‑150 (MQ=255)

                                                                                              gccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCTACACCGCCCCGGCCCTGGGCCGTGGCGAAGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑g                                               <  2:1752764/150‑1 (MQ=255)

                                                                                              gccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑g                                               <  1:461740/150‑1 (MQ=255)

                                                                                                cTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACACACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                  <  1:2315426/114‑1 (MQ=255)

                                                                                                cTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACACACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                  >  2:2315426/1‑114 (MQ=255)

                                                                                                 ttGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGTCTATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑gggg                                            >  1:1330124/1‑150 (MQ=255)

                                                                                                 ttGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑gggg                                            >  2:1678474/1‑150 (MQ=255)

                                                                                                     cAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGtt                                        <  2:1629571/150‑1 (MQ=255)

                                                                                                      aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaa                                                                               >  2:1690713/1‑111 (MQ=255)

                                                                                                      aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaa                                                                               <  1:1690713/111‑1 (MQ=255)

                                                                                                      aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGttt                                       <  2:1428694/150‑1 (MQ=255)

                                                                                                       gCCAGAGGGCCGGACAACCGGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGtttt                                      <  2:2218819/150‑1 (MQ=255)

                                                                                                        ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGttt                                       <  1:1019118/148‑1 (MQ=255)

                                                                                                        ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGttt                                       >  2:1019118/1‑148 (MQ=255)

                                                                                                        ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAATGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                       >  1:2154761/1‑117 (MQ=255)

                                                                                                         cAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCg                                                                                               <  1:1986083/92‑1 (MQ=255)

                                                                                                         cAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCg                                                                                               >  2:1986083/1‑92 (MQ=255)

                                                                                                          agagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                     <  1:1181696/117‑1 (MQ=255)

                                                                                                          agagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                     >  2:1181696/1‑117 (MQ=255)

                                                                                                                acGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCTATGGTTTCACGCACAGTGGTCATGCTCGAAAGCCTCTACGCCACAAGCGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTcaca                             >  1:1308711/2‑150 (MQ=255)

                                                                                                                 cGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAg                            <  1:1678474/150‑1 (MQ=255)

                                                                                                                        ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCg                     >  1:2108376/1‑150 (MQ=255)

                                                                                                                         cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTAt                                  >  2:1257825/1‑136 (MQ=255)

                                                                                                                         cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTAt                                  <  1:1257825/136‑1 (MQ=255)

                                                                                                                         cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGt                    >  1:1018968/1‑150 (MQ=255)

                                                                                                                         cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGt                    >  2:1802882/1‑150 (MQ=255)

                                                                                                                           gACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAt                  >  1:1554014/1‑150 (MQ=255)

                                                                                                                           gACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAt                  <  2:2108376/150‑1 (MQ=255)

                                                                                                                               tcgccgccacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc              <  2:1506476/143‑1 (MQ=255)

                                                                                                                                 tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc            <  2:619601/150‑1 (MQ=255)

                                                                                                                                   ccACACCGCCCCGGCCCTTGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAAGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt          >  2:1105986/1‑150 (MQ=255)

                                                                                                                                    cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc         <  2:278041/150‑1 (MQ=255)

                                                                                                                                     ccatcGCCACGGCCCAGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCa        >  2:200225/5‑150 (MQ=255)

                                                                                                                                     acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCa        >  1:835516/1‑150 (MQ=255)

                                                                                                                                     acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGAAAAAACGCCCC‑CTAGTGGGGCGtt                                        <  1:1515566/118‑1 (MQ=255)

                                                                                                                                      cacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACTACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAtt                 <  1:1055783/140‑1 (MQ=255)

                                                                                                                                      cacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACTACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAtt                 >  2:1055783/1‑140 (MQ=255)

                                                                                                                                      cacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAtt                 >  2:1055631/1‑140 (MQ=255)

                                                                                                                                      cacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAtt                 <  1:1055631/140‑1 (MQ=255)

                                                                                                                                       acCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc              <  2:1606438/142‑1 (MQ=255)

                                                                                                                                       acCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc              >  1:1606438/1‑142 (MQ=255)

                                                                                                                                        ccGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc     <  1:282320/150‑1 (MQ=255)

                                                                                                                                           ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGtttttgctat                                <  2:400869/120‑6 (MQ=38)

                                                                                                                                           ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGtttttgctat                                >  1:400869/1‑115 (MQ=38)

                                                                                                                                           ccacGGCCCAGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCACGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc              <  1:978038/135‑1 (MQ=255)

                                                                                                                                            cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGtttt                                      <  1:1882274/113‑1 (MQ=255)

                                                                                                                                            cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGtttt                                      >  2:1882274/1‑113 (MQ=255)

                                                                                                                                                 cccTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc  <  1:2093103/144‑1 (MQ=255)

                                                                                                                                                 cccTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc  >  2:2093103/1‑144 (MQ=255)

AATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGC  >  NC_002947/6191‑6479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`