BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,661,525	T→G	5.2%	V52G (GTC→GGC)	PP_1456 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,661,525	0	T	G	5.2%	306.9 / 6.5	106	V52G (GTC→GGC)	PP_1456	membrane protein
Reads supporting (aligned to +/- strand): ref base T (49/51); new base G (3/3); total (52/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 108 total aligned reads displayed.

CTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCTCA  >  NC_002947/1661376‑1661674

cTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGt                                                                                                                                                        <  2:881688/150‑1 (MQ=255)

 tATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTc                                                                                                                                                       >  1:1262087/1‑150 (MQ=255)

      gATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCAt                                                                                                                                                  >  1:1900877/1‑150 (MQ=255)

        ttGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCATGCCCGTTTTGGTCATGGCGCCCTGGTCGCCATGc                                                                                                                                                <  1:1354818/150‑1 (MQ=255)

           ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtgg                                                                                                                                             >  1:1205603/1‑150 (MQ=255)

           ccTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtgg                                                                                                                                             <  1:1993445/150‑1 (MQ=255)

            cTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggt                                                                                                                                            <  1:1943787/150‑1 (MQ=255)

             tACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggtg                                                                                                                                           >  2:2148197/1‑150 (MQ=255)

             tACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggtg                                                                                                                                           <  2:164631/150‑1 (MQ=255)

             tACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCtggtg                                                                                                                                           >  1:1294538/1‑150 (MQ=255)

                aTCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTTGCa                                                                                                                                        >  2:2111046/1‑150 (MQ=255)

                   agcagcGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTg                                                                                                                                     <  2:1064405/150‑1 (MQ=255)

                    gcagcGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGc                                                                                                                                                <  2:1635232/138‑1 (MQ=255)

                     cagcGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGt                                                                                                                                   <  1:1509774/150‑1 (MQ=255)

                        cGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCt                                                                                                                                <  1:1139538/150‑1 (MQ=255)

                        cGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCt                                                                                                                                <  2:1262087/150‑1 (MQ=255)

                          tAGTGCTGATCAACTACGCCTTCTCAAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGt                                                                                                                              <  2:1275072/150‑1 (MQ=255)

                             tGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCt                                                                                                                           <  1:687660/150‑1 (MQ=255)

                             tGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTACAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGTCACTGGTGCTGTCCt                                                                                                                           <  1:415645/150‑1 (MQ=255)

                              gCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGGCTGGGGCGGCCGGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCCTGGCGCCCTGGTCGCCATGCTGGTGACACTGGTGCTGTCCTa                                                                                                                          >  1:854610/1‑150 (MQ=255)

                                tGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATg                                                                                                                        >  2:2110503/1‑150 (MQ=255)

                                tGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATg                                                                                                                        <  2:1294538/150‑1 (MQ=255)

                                 gATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGt                                                                                                                       >  1:1855978/1‑150 (MQ=255)

                                 gATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGt                                                                                                                       <  2:209493/150‑1 (MQ=255)

                                        tACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCg                                                                                                                <  1:2107638/150‑1 (MQ=255)

                                            ccTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGc                                                                                                            >  1:1295648/1‑150 (MQ=255)

                                            ccTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTAGCCATGCTGTGGGCACTGGTGCTGTCCTATGTCACCTCGGAGc                                                                                                            >  2:382559/1‑150 (MQ=255)

                                              ttCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCGGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCg                                                                                                          >  1:2046315/1‑150 (MQ=255)

                                              ttCTCCAGTGCCCCGCATCTGGACATAATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCg                                                                                                          <  2:2046315/150‑1 (MQ=255)

                                                ctcCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGc                                                                                                        >  1:592732/1‑150 (MQ=255)

                                                   cAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCAt                                                                                                     >  1:832687/1‑150 (MQ=255)

                                                        ccccGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑ccc                                                                                               >  1:819674/1‑150 (MQ=255)

                                                         cccGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCt                                                                                              <  2:1178650/150‑1 (MQ=255)

                                                          ccGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTAt                                                                                                                         >  1:930415/1‑123 (MQ=255)

                                                          ccGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTg                                                                                             <  2:1947332/150‑1 (MQ=255)

                                                          ccGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGACACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTg                                                                                             >  2:1682086/1‑150 (MQ=255)

                                                                cTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAgc                                                                                       >  2:164510/1‑150 (MQ=255)

                                                                cTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAgc                                                                                       <  1:382559/150‑1 (MQ=255)

                                                                  ggACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTGTGTCACCTCGGAGCCGGCGATCG‑CCCTGGCCAgcgc                                                                                     >  2:2133817/1‑150 (MQ=255)

                                                                   gACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGcc                                                                                    >  2:1835997/1‑150 (MQ=255)

                                                                   gACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTGCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCAATGCTGGCGGCACTGGTGCTGTCCTATCCCACCTCGGACCCGGCCATCG‑CCCTGGCCAGCGcc                                                                                    >  2:1311021/1‑150 (MQ=255)

                                                                     catcatCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCAc                                                                                  >  2:206344/1‑150 (MQ=255)

                                                                      atcatcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACt                                                                                 <  2:1900877/150‑1 (MQ=255)

                                                                         atcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCg                                                                              >  2:1245015/1‑150 (MQ=255)

                                                                         atcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCg                                                                              >  1:1828868/1‑150 (MQ=255)

                                                                          tcTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGt                                                                             >  2:194387/1‑150 (MQ=255)

                                                                            tGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGttc                                                                           >  1:106759/1‑150 (MQ=255)

                                                                            tGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGttc                                                                           >  1:226578/1‑150 (MQ=255)

                                                                              gTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCt                                                                                                                           <  2:343761/101‑1 (MQ=255)

                                                                              gTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCt                                                                                                                           >  1:343761/1‑101 (MQ=255)

                                                                                 tGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCAt                                                                      <  2:1794027/150‑1 (MQ=255)

                                                                                        ggcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAg                                                               <  1:194387/150‑1 (MQ=255)

                                                                                         gcggcCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGc                                                              <  1:1835997/150‑1 (MQ=255)

                                                                                              cTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATc                                                         >  1:135031/1‑150 (MQ=255)

                                                                                                 gtgtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCACCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCt                                                                                                                  <  2:1162802/91‑1 (MQ=255)

                                                                                                 gtgtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCACCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCt                                                                                                                  >  1:1162802/1‑91 (MQ=255)

                                                                                                  tgtTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGAct                                                     <  2:135031/150‑1 (MQ=255)

                                                                                                    ttCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATTGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGATGTCCTATGTCACCTCCGGGCAGGCC‑TCGCCCCTGGTCACCCCCACTGCGTTCTCCATTTCCGAGCTGATCGActgg                                                   >  2:2260543/1‑150 (MQ=255)

                                                                                                    ttCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActgg                                                   <  2:846243/150‑1 (MQ=255)

                                                                                                    ttCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActgg                                                   >  2:580849/1‑150 (MQ=255)

                                                                                                    ttCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActgg                                                   >  1:864747/1‑150 (MQ=255)

                                                                                                        tCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActggctgg                                               <  2:864747/150‑1 (MQ=255)

                                                                                                          cTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGgtg                                             <  2:1722085/150‑1 (MQ=255)

                                                                                                             cgcgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTc                                          <  1:1682086/150‑1 (MQ=255)

                                                                                                              gcgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                         <  2:1569102/150‑1 (MQ=255)

                                                                                                              gcgACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCa                                         <  2:1828868/150‑1 (MQ=255)

                                                                                                                 aCATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCa                                      <  2:832687/150‑1 (MQ=255)

                                                                                                                 aCATGGTGCACATCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCa                                      <  1:2111046/150‑1 (MQ=255)

                                                                                                                    tGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCa                                   <  1:1735447/150‑1 (MQ=255)

                                                                                                                     ggTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAc                                  <  1:1245015/150‑1 (MQ=255)

                                                                                                                      gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCCACG‑CCCTGGCCAGCGCCACTGCGttc                                                                           >  1:1312713/1‑108 (MQ=255)

                                                                                                                      gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGttc                                                                           <  2:1312713/108‑1 (MQ=255)

                                                                                                                      gTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAcc                                 >  1:2235834/1‑150 (MQ=255)

                                                                                                                        gCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActgg                                                   >  2:2167714/1‑130 (MQ=255)

                                                                                                                        gCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGActgg                                                   <  1:2167714/130‑1 (MQ=255)

                                                                                                                         cAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGt                              <  1:2110503/150‑1 (MQ=255)

                                                                                                                          aGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAg                                                               <  2:410589/116‑1 (MQ=255)

                                                                                                                            aCCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCgg                           >  1:563254/1‑150 (MQ=255)

                                                                                                                              ccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTc                                                                                                                             <  2:1830525/51‑1 (MQ=255)

                                                                                                                              ccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTc                                                                                                                             >  1:1830525/1‑51 (MQ=255)

                                                                                                                              ccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGtt                                                                            >  2:365838/1‑99 (MQ=255)

                                                                                                                              ccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGtt                                                                            <  1:365838/99‑1 (MQ=255)

                                                                                                                              ccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGcc                         >  1:2106795/1‑150 (MQ=255)

                                                                                                                              ccGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGcc                         >  2:1945286/1‑150 (MQ=255)

                                                                                                                                gTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGc                                       >  2:598323/1‑134 (MQ=255)

                                                                                                                                gTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGc                                       <  1:598323/134‑1 (MQ=255)

                                                                                                                                   ttGGTCAGGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCGGTCCTATGTCACCTCGGCGCCGGACATCG‑AGCACGCCAGCGCCACTGCGTTTTTCATTTCCGAGCTGACCGACTGGCTGGTGTTCAGCATCACCCGGCGGCCGCTgc                    <  1:281372/150‑1 (MQ=255)

                                                                                                                                     gggcagggCACCCTGGGCGCCATGCTGGTGGAACTGGTGCTGTCCTATGTCACGTCGGAGCCGGCCATCG‑CCCTGGCCAGTCCCACTGCGTTCTTCATTTCCGAGCTGGTCGACTGGATGGTGGTCAGCATCACCCGCCGACCGCTgcgc                  <  2:854610/144‑1 (MQ=255)

                                                                                                                                      agcATGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGCGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGcgcg                 >  2:1706117/3‑150 (MQ=255)

                                                                                                                                       tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGTCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  2:1420974/1‑150 (MQ=255)

                                                                                                                                       tCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGa                >  1:179813/1‑150 (MQ=255)

                                                                                                                                         aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCAt                                     <  2:66336/127‑1 (MQ=255)

                                                                                                                                         aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCAt                                     >  1:66336/1‑127 (MQ=255)

                                                                                                                                         aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAc                                  <  2:1165399/130‑1 (MQ=255)

                                                                                                                                         aTGGCGACCAGGGCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCAc                                  >  1:1165399/1‑130 (MQ=255)

                                                                                                                                           ggCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGc            <  1:2260543/150‑1 (MQ=255)

                                                                                                                                              gcCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCtg         >  1:1830216/1‑150 (MQ=255)

                                                                                                                                                 cTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATTGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTgg      <  2:1493324/150‑1 (MQ=255)

                                                                                                                                                 cTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTgg      <  2:118974/150‑1 (MQ=255)

                                                                                                                                                     tCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCTCa  <  2:1295648/150‑1 (MQ=255)

CTATCTGATTGCCTACATCAGCAGCGTAGTGCTGATCAACTACGCCTTCTCCAGTGCCCCGCATCTGGACATCATCTGGTCTGCCTGGGGCGGCCTGGTGTTCATCCTGCGCGACATGGTGCAGACCCGTTTTGGTCATGGCGCCCTGGTCGCCATGCTGGTGGCACTGGTGCTGTCCTATGTCACCTCGGAGCCGGCCATCG‑CCCTGGCCAGCGCCACTGCGTTCTTCATTTCCGAGCTGATCGACTGGCTGGTGTTCAGCATCACCCGTCGGCCGCTGCGCGACCGCCTGTGGCTCA  >  NC_002947/1661376‑1661674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`