Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,457,043 T→A 5.1% L24Q (CTG→CAG)  PP_2150 → thiamine biosynthesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,457,0430TA 5.1% 365.4 / 6.1 118L24Q (CTG→CAG) PP_2150thiamine biosynthesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (63/49);  new base A (3/3);  total (66/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 122 total aligned reads displayed.

CTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGTAA  >  NC_002947/2456896‑2457192
                                                                                                                                                   |                                                                                                                                                     
cTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGCCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                     <  1:1780796/150‑1 (MQ=255)
  aCAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggt                                                                                                                                                   >  1:215711/1‑150 (MQ=255)
   cAGGATTATTGTTTTTCCTGTACGAGCGTATTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtg                                                                                                                                                  <  2:295467/150‑1 (MQ=255)
    aGGATTATCGCTGCCCCTGTGGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtgg                                                                                                                                                 >  2:1022439/1‑150 (MQ=255)
        ttATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                     >  1:1070543/1‑142 (MQ=255)
        ttATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                     <  2:1070543/142‑1 (MQ=255)
        ttATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCg                                                                                                                                             >  1:386342/1‑150 (MQ=255)
           tCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGAcc                                                                                                                                          >  1:101827/1‑150 (MQ=255)
             gCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCAGGCGCTCCAGggtggg                                                                                                                                                >  2:173423/1‑141 (MQ=255)
             gCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCAGGCGCTCCAGggtggg                                                                                                                                                <  1:173423/142‑2 (MQ=38)
              cTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATg                                                                                                                                       <  1:580204/150‑1 (MQ=255)
               tGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATgg                                                                                                                                      <  1:1418541/150‑1 (MQ=255)
                  cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCAt                                                                                                                                        >  2:904514/1‑145 (MQ=255)
                  cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCAt                                                                                                                                        <  1:904514/145‑1 (MQ=255)
                  cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCAACACACCTCCTGCACCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgca                                                                                                                                   >  2:1716353/1‑150 (MQ=255)
                    cTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgcagc                                                                                                                                 <  1:1320262/150‑1 (MQ=255)
                     tGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                                     >  2:2022116/1‑145 (MQ=255)
                     tGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATggg                                                                                                                                     <  1:2022116/145‑1 (MQ=255)
                       tAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCagcagc                                                                                                                              >  1:251282/1‑150 (MQ=255)
                        aGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTGCTGTGCATGCTGCTGCTACTCACCGCCTGAAACCAAGCCCCCACCCTGGAGCGCCTGGGTGATCCTACCATCGGCAGCAGCt                                                                                                                             >  2:346761/1‑150 (MQ=38)
                             cGGGGTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGCTCATTCTGCTGCCACTCACCGCCTGCAACCAACGCCCCACCCTGGCGCGCCTGGGGGGTCCCACCATGGCCACCAGCGACAGc                                                                                                                        >  2:716792/1‑150 (MQ=255)
                                  ttACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCa                                                                                                                   <  2:697951/150‑1 (MQ=255)
                                    aCCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGt                                                                                                                 <  1:2170561/150‑1 (MQ=255)
                                     cccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCGACTTTGCGCACATCCCTACTGTTCATTCTGCTGCGACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACGGCATCCAGTa                                                                                                                >  1:1332624/1‑150 (MQ=255)
                                      ccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCAt                                                                                                                                        >  1:2114159/1‑125 (MQ=255)
                                      ccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTAc                                                                                                               >  1:807056/1‑150 (MQ=255)
                                      ccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTAc                                                                                                               >  1:687301/1‑150 (MQ=255)
                                             gagGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTggg                                                                                                                                                    <  1:1858671/106‑1 (MQ=255)
                                                gCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTa                                                                                                                            >  2:1434311/1‑127 (MQ=255)
                                                gCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTa                                                                                                                            <  1:1434311/127‑1 (MQ=255)
                                                gCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTcc                                                                                                           <  2:258414/144‑1 (MQ=255)
                                                gCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTcc                                                                                                           >  1:258414/1‑144 (MQ=255)
                                                  cAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATc                                                                                                                     <  2:1546948/132‑1 (MQ=255)
                                                  cAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATc                                                                                                                     >  1:1546948/1‑132 (MQ=255)
                                                   aGCACCGGCACCCGAGAAACCCTACTGTGCGCACAGCCCGACGGTCCATGCTGCTGCTACTAACCGCCTGCAACCGAGGCCCCCCCCTGGAGCGCCTGGGTGGTCCGACCATGGCCCGCAGCTACCGCCTCCATGCCGTCGCCGAGCCtg                                                                                                  >  2:836930/1‑150 (MQ=255)
                                                      aCCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtggtg                                                                                               <  1:1022439/150‑1 (MQ=255)
                                                       ccGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCcgc                                                                                                         <  2:779357/139‑1 (MQ=255)
                                                       ccGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCcgc                                                                                                         >  1:779357/1‑139 (MQ=255)
                                                        cGGCAACCGGGAAACCCTACTGTGCGCACAGCGCTACTGTTCATCCTGCTGCTACTCACCGCCTGCAGCGAAGGCCCCACCCTGGGGCGCCTGGGGGGTACGAGCATGGACTTGTGCTACAGCAGGCGGTAGGTGCGCGAGCCtggtgac                                                                                             >  2:491976/1‑148 (MQ=255)
                                                         ggCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGcc                                                                                            <  1:1997936/150‑1 (MQ=255)
                                                          gCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGccc                                                                                           >  2:1980688/1‑150 (MQ=255)
                                                               cGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAg                                                                                                                  <  1:675498/122‑1 (MQ=255)
                                                               cGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAg                                                                                                                  >  2:675498/1‑122 (MQ=255)
                                                               cGAGAAACCCTACTGTGCGCACAGCCCTACGTTTCATTCTGCTGCTACGCACCGCCTGCAACCAAGGCCCCACCCTTGATCGCCTGGGTCGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCTCGCTGc                                                                                      <  2:620381/150‑1 (MQ=255)
                                                                gagaAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTCAATCATCCCGTACGTCCGCGAGCCTGGTGGCCCGCTGcc                                                                                     >  1:123977/1‑150 (MQ=255)
                                                                     aCCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGAAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGccc                                                                                >  2:1478782/1‑150 (MQ=255)
                                                                       ccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCcgc                                                                                                         <  1:1040609/123‑1 (MQ=255)
                                                                       ccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAg                                                                              <  2:1957132/150‑1 (MQ=255)
                                                                       ccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAg                                                                              <  2:2329793/150‑1 (MQ=255)
                                                                         tACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGt                                                                            >  1:860088/1‑150 (MQ=255)
                                                                           ctttgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGc                                                                          <  1:1980688/147‑1 (MQ=255)
                                                                           cTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTCCGGCAGCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGc                                                                          <  2:327160/150‑1 (MQ=255)
                                                                             gtgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAg                                                                        >  1:1140243/1‑150 (MQ=255)
                                                                              tgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGa                                                                       >  2:668230/1‑150 (MQ=255)
                                                                              tgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGGACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGa                                                                       >  1:2273445/1‑150 (MQ=255)
                                                                                  cacaGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGc                                                                   >  1:1603888/1‑150 (MQ=255)
                                                                                   acaGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCg                                                                  <  2:386342/150‑1 (MQ=255)
                                                                                     aGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGt                                                                <  2:101827/150‑1 (MQ=255)
                                                                                     aGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGt                                                                >  2:687288/1‑150 (MQ=255)
                                                                                      gCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTg                                                               <  1:687288/150‑1 (MQ=255)
                                                                                       cccTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCGGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTgg                                                              <  2:215711/150‑1 (MQ=255)
                                                                                         cTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGAAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaa                                                            <  1:1478782/150‑1 (MQ=255)
                                                                                          tACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                                           >  2:608550/1‑150 (MQ=255)
                                                                                           aCTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGACTCGTGCCCCGCGGCCGGCCCAGGTGCAGATGGCGGTGGAAAc                                                          >  2:1939441/1‑150 (MQ=255)
                                                                                               ttCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                      <  2:1758195/150‑1 (MQ=255)
                                                                                                 cATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCACGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCt                                                    <  2:2151436/150‑1 (MQ=255)
                                                                                                  aTTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCgg                                                                                   <  2:2158640/118‑1 (MQ=255)
                                                                                                  aTTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                                           >  2:922579/1‑142 (MQ=255)
                                                                                                  aTTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                                           <  1:922579/142‑1 (MQ=255)
                                                                                                  aTTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTg                                                   >  2:1933437/1‑150 (MQ=255)
                                                                                                  aTTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTg                                                   >  2:2317557/1‑150 (MQ=255)
                                                                                                  aTTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAACATCCAGTCCGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGCGCAGAGGCCGGTGGAAACCATCCTg                                                   >  1:41050/1‑150 (MQ=255)
                                                                                                        ctgctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAg                                                                                                                  <  2:894970/81‑1 (MQ=255)
                                                                                                             aCTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTg                                                               <  2:335030/127‑1 (MQ=255)
                                                                                                              cTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGac                                       <  2:251282/150‑1 (MQ=255)
                                                                                                                cACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGAAGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGCCCCGCTGCCGGCCCAGGTGCAGATCGCGGTGGGAACCATCCTGCACGACATCCaccc                                     >  2:1189730/1‑149 (MQ=255)
                                                                                                                 aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGc                                                                                  <  1:452725/104‑1 (MQ=255)
                                                                                                                 aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGc                                                                                  >  2:452725/1‑104 (MQ=255)
                                                                                                                 aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGaccac                                    >  2:1607619/1‑150 (MQ=255)
                                                                                                                 aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGaccac                                    <  2:1451592/150‑1 (MQ=255)
                                                                                                                           aCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGa                          >  1:2210577/1‑150 (MQ=255)
                                                                                                                            ccAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACAACTACTCgtc                         <  2:807056/150‑3 (MQ=255)
                                                                                                                              aaGGCCCCACCCTGGAGCGCCTGGGTGGCCCGACCATGGCCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCCGCTCGCGGACGACACCACCCCGCACGCCATCGCCCCCCACTACTCCACCt                       >  2:1774973/1‑150 (MQ=255)
                                                                                                                                ggCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTAc                     <  1:757411/150‑1 (MQ=255)
                                                                                                                                 ggaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTAcc                    >  1:397699/4‑150 (MQ=255)
                                                                                                                                  gaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTc                            >  2:1637203/3‑141 (MQ=255)
                                                                                                                                  gaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTc                            <  1:1637203/139‑1 (MQ=255)
                                                                                                                                   cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                      <  2:882940/114‑1 (MQ=255)
                                                                                                                                   cccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                      >  1:882940/1‑114 (MQ=255)
                                                                                                                                    ccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcgcg                 >  2:5984/1‑150 (MQ=255)
                                                                                                                                    ccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcgcg                 >  2:1454937/1‑150 (MQ=255)
                                                                                                                                     cACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCgcgg                >  2:2170727/1‑150 (MQ=255)
                                                                                                                                        ccTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATc                                                                                                                     <  1:636275/46‑1 (MQ=255)
                                                                                                                                          tGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCAAGACATCGACCACCACTACTCGACCTACCGCGGCGAtt           >  1:1956600/1‑150 (MQ=255)
                                                                                                                                              gcgcCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACAGCGACCACCACTACTCGACCTACCGCGGCGATTCCAt       >  1:2207172/1‑150 (MQ=255)
                                                                                                                                                  cTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACAACCACTACTCGACCTACCGCGGCGATTCCATCGTa   >  1:2167548/1‑150 (MQ=255)
                                                                                                                                                   tGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGTaa  <  1:2317557/150‑1 (MQ=255)
                                                                                                                                                   tGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGTaa  <  2:673944/150‑1 (MQ=255)
                                                                                                                                                   tGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGTaa  <  2:1522305/150‑1 (MQ=255)
                                                                                                                                                   tGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTAAGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGTaa  >  1:2226792/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATCGTAA  >  NC_002947/2456896‑2457192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑