Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,873,691 T→C 6.1% V143A (GTG→GCG) ‡ cigR → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,873,6910TC 6.1% 244.5 / 3.0 81V143A (GTG→GCG) ‡cigRmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (38/38);  new base C (2/3);  total (40/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACC  >  NC_002947/2873544‑2873837
                                                                                                                                                   |                                                                                                                                                   
cTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                                   >  1:1482808/1‑150 (MQ=255)
cTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                                   <  2:1485714/150‑1 (MQ=255)
 tCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGc                                                                                                                                                  <  1:1085358/150‑1 (MQ=255)
     ggCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCGGACCTGATTCTGGTCGCCGTGGCGAcc                                                                                                                                              >  2:1214304/1‑150 (MQ=255)
      gCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCACGGCGAcca                                                                                                                                             <  1:1541887/150‑2 (MQ=255)
        aTTCAGAAGAACCGGGCACGCCGCAAGCCCCTGCCACCTCGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCggg                                                                                                                                           <  2:124051/150‑1 (MQ=255)
         ttCACAAGAAACTGGCCCGCGGCAAGTCCCGGCCACCTGGTACCGCCAAGAAGCTGGATGGTCTTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCCGGTCGCCACGGCGAccagaa                                                                                                                                          <  2:2124018/150‑5 (MQ=255)
           cAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGAatc                                                                                                                                        <  2:100573/150‑1 (MQ=255)
            agaagaACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGgtca                                                                                                                                       >  1:471019/1‑150 (MQ=255)
                 aaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                                  <  1:1156116/150‑1 (MQ=255)
                 aaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                                  <  2:2198405/150‑1 (MQ=255)
                 aaCCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGATGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCGGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTAt                                                                                                                                  <  1:1214304/150‑1 (MQ=255)
                   ccTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGa                                                                                                                                >  1:1634099/1‑150 (MQ=255)
                   ccTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGAGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGa                                                                                                                                >  2:521796/1‑150 (MQ=255)
                    cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                               <  1:686835/150‑1 (MQ=255)
                    cTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAg                                                                                                                               <  2:2053177/150‑1 (MQ=255)
                      ggCCCGCGGCAAGCCACAGCCACCGGGCATCGCGAAGGAGATGAGTGGAGCGCTATTTGTGCATCTGCCTCGCTACCACGTGAATGAAAGAATAAAAGCGGGCGCAGACCTGATTCTGGTTGCCGTGGCGACCGCGATCATCTATGAgtt                                                                                                                             >  2:10708/1‑148 (MQ=255)
                         ccGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGtt                                                                                                                          >  2:158930/1‑150 (MQ=255)
                            gggCAAGCCCCTGCCTCCTGGTATCGCCAAGAAGATGGATGGTCGTCTGTTGGGGCATCTGCCTCACTACGGCGGGTATGAGTGGATGCAAGCGCGCGCAGAGCTGATTCTGGTCGCCGTGCCGACCGGGATCATCTATGAGGTCTTGaa                                                                                                                       <  2:758756/149‑1 (MQ=255)
                               cAAGCCCCTGCCACCTGGTATCTCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgg                                                                                                                    <  2:920113/150‑1 (MQ=255)
                               cAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgg                                                                                                                    <  2:1482808/150‑1 (MQ=255)
                                aaGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGGCGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACggg                                                                                                                   <  2:410927/150‑1 (MQ=255)
                                 aGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACgggg                                                                                                                  <  2:1849729/150‑1 (MQ=255)
                                     ccTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATTTATGAGGTGTTGAACGGGGCAtt                                                                                                              <  1:1445545/150‑1 (MQ=255)
                                        gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                           <  2:1424296/150‑1 (MQ=255)
                                        gCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGGGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGGTGAACGGGGCATTCGa                                                                                                           <  2:237778/150‑1 (MQ=255)
                                          cACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACt                                                                                                         >  1:330598/1‑150 (MQ=255)
                                            ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGTCATTCGACTaa                                                                                                       <  2:232745/150‑1 (MQ=255)
                                            ccTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTaa                                                                                                       <  2:330598/150‑1 (MQ=255)
                                               ggTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGc                                                                                                    <  1:158930/150‑1 (MQ=255)
                                                gTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCACGCGGGTGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGcc                                                                                                   <  2:1356293/150‑1 (MQ=255)
                                                     gCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                                   <  2:2263351/97‑1 (MQ=255)
                                                     gCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTgg                                                                                                                                                   >  1:2263351/1‑97 (MQ=255)
                                                     gCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAgg                                                                                              >  2:757344/1‑150 (MQ=255)
                                                      ccAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGt                                                                                             >  1:308075/1‑150 (MQ=255)
                                                       cAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGGGTTGAACGGGGCATTCGACTAAGGCCGCAGGTg                                                                                            >  1:1302622/1‑150 (MQ=255)
                                                        aagaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGc                                                                                           >  2:472587/1‑150 (MQ=255)
                                                          gaagCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGa                                                                                         <  1:1160927/150‑1 (MQ=255)
                                                             gCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACg                                                                                                                     >  2:1669942/1‑119 (MQ=255)
                                                             gCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACg                                                                                                                     <  1:1669942/119‑1 (MQ=255)
                                                               tGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGt                                                                                    <  2:1718818/150‑1 (MQ=255)
                                                                      tcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcat                                                                                                                                      <  2:2069966/93‑1 (MQ=255)
                                                                      tcgtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGatcat                                                                                                                                      >  1:2069966/1‑93 (MQ=255)
                                                                        gtcTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCCGCCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGCTCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGt                                                                           >  1:425743/1‑150 (MQ=255)
                                                                            gTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCt                                                                       <  2:471019/150‑1 (MQ=255)
                                                                             ttGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGGGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGGTCGGTCCCTg                                                                      <  2:1148404/150‑1 (MQ=255)
                                                                                  gCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGgc                                                                 >  1:387108/1‑150 (MQ=255)
                                                                                           tCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTg                                                        >  2:159203/1‑150 (MQ=255)
                                                                                           tCACTACGACGGGTATGAGAGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCGTTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCCTg                                                        >  2:159307/1‑150 (MQ=255)
                                                                                             aCTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGcc                                                      >  1:849181/1‑150 (MQ=255)
                                                                                              cTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCt                                                     >  1:1993739/1‑150 (MQ=255)
                                                                                                   tggggTATGGCTGGATGCAAGCGGGCGGAGACCTGATTCTGGTCGCCGTGGCGGCCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGGGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGAt                                                <  2:1302622/148‑1 (MQ=255)
                                                                                                      ggTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTggg                                             >  2:846260/1‑150 (MQ=255)
                                                                                                      ggTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATATATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATggg                                             >  2:2015703/1‑150 (MQ=255)
                                                                                                        tATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCt                                           <  1:472587/150‑1 (MQ=255)
                                                                                                          tGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCt                                                                                                                                    >  2:1518105/1‑59 (MQ=255)
                                                                                                          tGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCt                                                                                                                                    <  1:1518105/59‑1 (MQ=255)
                                                                                                             gTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCt                                                                       >  2:284950/1‑117 (MQ=255)
                                                                                                             gTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGAGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCt                                                                       <  1:284950/117‑1 (MQ=255)
                                                                                                                   gCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGTAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGcc                               <  1:757344/150‑1 (MQ=255)
                                                                                                                   gCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGcc                               <  2:308075/150‑1 (MQ=255)
                                                                                                                     aaGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTAGGTACATGCAGGCGCTAGCGGGCCTGCGTTGGGCTGC‑TGCGCAGccag                             >  2:843252/1‑148 (MQ=255)
                                                                                                                       gCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAAc                           >  1:2133765/1‑150 (MQ=255)
                                                                                                                        cGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACg                          >  1:907819/1‑150 (MQ=255)
                                                                                                                           gcgcAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGTTGGGCTGCTGC‑GCAGCCCatcgcag                       <  1:846260/150‑7 (MQ=255)
                                                                                                                               aGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATc                   >  2:52409/1‑150 (MQ=255)
                                                                                                                                 aCCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCaa                 >  2:1305748/1‑150 (MQ=255)
                                                                                                                                    tgatcccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGc              <  1:905092/143‑1 (MQ=255)
                                                                                                                                    tgatcccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGc              >  1:1749868/8‑150 (MQ=255)
                                                                                                                                      aTTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTg            <  2:1993739/150‑1 (MQ=255)
                                                                                                                                      aTTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTg            >  2:244415/1‑150 (MQ=255)
                                                                                                                                       tcccggTCGCCACGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGCCCTATCAACGCTGc           >  1:1933653/5‑150 (MQ=255)
                                                                                                                                          tGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGt        >  1:455219/1‑150 (MQ=255)
                                                                                                                                            gTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTaa      <  1:2186240/150‑1 (MQ=255)
                                                                                                                                            gTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAAAGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTaa      >  1:1946397/1‑150 (MQ=255)
                                                                                                                                               gCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAacac   >  1:1722906/1‑150 (MQ=255)
                                                                                                                                               gCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCCGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAacac   >  1:817140/1‑150 (MQ=255)
                                                                                                                                                ccGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                           <  2:111639/46‑1 (MQ=255)
                                                                                                                                                ccGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGa                                                                                                           >  1:111639/1‑46 (MQ=255)
                                                                                                                                                ccGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACAcc  >  2:294310/1‑150 (MQ=255)
                                                                                                                                                ccGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACGAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACAcc  >  2:43033/1‑150 (MQ=255)
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CTCCAGGCATTCAGAAGAACCTGGCCCGCGGCAAGCCCCTGCCACCTGGTATCGCCAAGAAGCTGGATGGTCGTCTGTTGGGGCAGCTGCCTCACTACGACGGGTATGAGTGGATGCAAGCGGGCGCAGACCTGATTCTGGTCGCCGTGGCGACCGGGATCATCTATGAGGTGTTGAACGGGGCATTCGACTAAGGCCGCAGGTGCGAGCAGTCCGCTCGGTCCCTGCAGGCGCTGGCGTGCCTGCGATGGGCTGC‑GCAGCAGCCCAACGGCCTATCAACGCTGCCGTAACACC  >  NC_002947/2873544‑2873837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑