BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,166,099	G→C	5.9%	G163A (GGC→GCC)	PP_2780 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,166,099	0	G	C	5.9%	382.4 / 13.0	119	G163A (GGC→GCC)	PP_2780	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand): ref base G (59/53); new base C (3/4); total (62/57)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01

Only 100 of 120 total aligned reads displayed.

TCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACA  >  NC_002947/3165950‑3166245

tCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATgg                                                                                                                                                    >  2:9917/1‑150 (MQ=255)

   tCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCAt                                                                                                                                                 >  2:1465901/1‑150 (MQ=255)

   tCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCAt                                                                                                                                                 >  1:1869432/1‑150 (MQ=255)

         gCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAg                                                                                                                                           >  1:375590/1‑150 (MQ=255)

         gCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaa                                                                                                                                           <  1:1510968/150‑2 (MQ=255)

          cAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGt                                                                                                                                          <  2:151762/150‑1 (MQ=255)

           aGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTg                                                                                                                                         >  2:1497594/1‑150 (MQ=255)

           aGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTg                                                                                                                                         >  2:1950519/1‑150 (MQ=255)

           aGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTg                                                                                                                                         >  1:1845247/1‑150 (MQ=255)

            ggACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGACTGCCGATGGCATCGGCAGTGa                                                                                                                                        >  1:416422/1‑150 (MQ=255)

               cATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaatcaccc                                                                                                                                     <  2:481239/150‑8 (MQ=255)

                 tgctgcTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGcc                                                                                                                                   <  2:2308077/150‑1 (MQ=255)

                  gctgctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGccc                                                                                                                                  >  2:1911983/1‑150 (MQ=255)

                   ctgctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCg                                                                                                                                 <  1:327965/150‑1 (MQ=255)

                         aGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATc                                                                                                                           <  2:770008/150‑1 (MQ=255)

                            cTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAAt                                                                                                                        <  1:1996531/150‑1 (MQ=255)

                             tGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGccc                                                                                                                                  <  1:666560/139‑1 (MQ=255)

                             tGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGccc                                                                                                                                  >  2:666560/1‑139 (MQ=255)

                                       cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCa                                                                                                                                            <  1:364238/119‑1 (MQ=255)

                                       cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCa                                                                                                                                            >  2:364238/1‑119 (MQ=255)

                                       cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                             >  2:561527/1‑150 (MQ=255)

                                        ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGGCCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAg                                                                                                                                      <  1:411758/124‑1 (MQ=255)

                                        ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGGCCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAg                                                                                                                                      >  2:411758/1‑124 (MQ=255)

                                        ccGGCGCAGGCGCCGTCCTGGGATCAATTGAGGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                            >  1:62807/1‑150 (MQ=255)

                                        caggCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                            >  1:390004/3‑150 (MQ=255)

                                              cAGGCGCCGTCCTGGGTTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCGTGCGCGGCTGATTGCCGATGGCATCGGCAGTGAACGGCCGGTGATCACTGTCAGCTGCGCCTGcgcg                                                                                                      <  2:759215/150‑1 (MQ=255)

                                               aGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCgg                                                                                                     <  2:1869432/150‑1 (MQ=255)

                                                 gcgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGa                                                                                                   >  1:993169/1‑150 (MQ=255)

                                                    ccGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCg                                                                                                >  1:198261/1‑150 (MQ=255)

                                                     cGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGt                                                                                               >  2:8324/1‑150 (MQ=255)

                                                      gTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTc                                                                                              >  2:1207533/1‑150 (MQ=255)

                                                       tCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTcc                                                                                             >  1:1337209/1‑150 (MQ=255)

                                                       tCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTcc                                                                                             >  1:1629008/1‑150 (MQ=255)

                                                          tGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAg                                                                                          >  2:2042046/1‑150 (MQ=255)

                                                              cTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCa                                                                                      >  2:890754/1‑150 (MQ=255)

                                                              cTCAATTGATGGCGAGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCa                                                                                      >  1:1484886/1‑150 (MQ=255)

                                                                 aaTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCAt                                                                                                                                                 <  1:1976245/88‑1 (MQ=255)

                                                                 aaTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCAt                                                                                                                                                 >  2:1976245/1‑88 (MQ=255)

                                                                  aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCgg                                                                                  <  2:1629008/150‑1 (MQ=255)

                                                                  aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCgg                                                                                  >  2:933528/1‑150 (MQ=255)

                                                                     gATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCaa                                                                               <  2:1284044/150‑1 (MQ=255)

                                                                         gcgcGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCa                                                                           >  1:2215040/1‑150 (MQ=255)

                                                                           gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                         <  2:67109/150‑1 (MQ=255)

                                                                           gcgACAGGCGCAGGTCGATCCTGACAGATCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGAGAGCGTCCCAGGCCATCGGCAATgagtt                                                                         >  1:574450/1‑146 (MQ=255)

                                                                              aCAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                      <  1:933528/150‑1 (MQ=255)

                                                                              aCAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                      <  1:1207533/150‑1 (MQ=255)

                                                                                   cgcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgatga                                                                 <  1:2042046/150‑1 (MQ=255)

                                                                                    gcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAg                                                                                          >  2:1522488/1‑124 (MQ=255)

                                                                                     cAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATc                                                               <  1:561527/150‑1 (MQ=255)

                                                                                      aGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGAGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATcc                                                              >  2:1133721/1‑150 (MQ=255)

                                                                                        ggcGATCCTGACAGGTCACGGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTAAGCAATTTCAGCAGCGCCTGCGCGGGAGCGTCCCAAGCCATCGGCAATGCATTCCAGATGATCCGt                                                            <  2:375590/148‑1 (MQ=255)

                                                                                         tCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGTATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTc                                                           >  2:301685/1‑150 (MQ=255)

                                                                                             tCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCgcgg                                                       >  2:28499/1‑150 (MQ=255)

                                                                                               cTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCgcggcg                                                     >  1:1370877/1‑150 (MQ=255)

                                                                                                tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAAt                                                                                                                        >  1:555797/1‑82 (MQ=255)

                                                                                                tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTca                                                                                                                    >  1:25791/1‑86 (MQ=255)

                                                                                                tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGa                                                    <  1:1164478/150‑1 (MQ=255)

                                                                                                 gACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAg                                                   <  1:9917/150‑1 (MQ=255)

                                                                                                 gACAGTTAACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGAGTCCCAGGCCATCGGCAATGAATTGCAGATGATCCGTTGCGGagag                                                   >  1:1898997/1‑150 (MQ=255)

                                                                                                        cACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAgg                                            >  2:417907/1‑149 (MQ=255)

                                                                                                        cACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGc                                            <  2:1211834/150‑1 (MQ=255)

                                                                                                        cACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGc                                            <  2:1337209/150‑1 (MQ=255)

                                                                                                        cACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGc                                            <  1:8324/150‑1 (MQ=255)

                                                                                                         aCTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGc                                                                                                 <  1:938639/96‑1 (MQ=255)

                                                                                                         aCTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGc                                                                                                 >  2:938639/1‑96 (MQ=255)

                                                                                                         aCTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCt                                           <  2:2287387/150‑1 (MQ=255)

                                                                                                          cTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATTGGCAATGCATTCCa                                                                      >  1:561858/1‑122 (MQ=255)

                                                                                                          cTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATTGGCAATGCATTCCa                                                                      <  2:561858/122‑1 (MQ=255)

                                                                                                          cTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGAGGCGAGGTCGAGCTg                                          >  2:2175682/1‑150 (MQ=255)

                                                                                                                 agagCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTg                                   >  1:241904/1‑150 (MQ=255)

                                                                                                                   agCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGcc                                 >  1:982793/1‑150 (MQ=255)

                                                                                                                     cAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgg                               >  1:880719/1‑150 (MQ=255)

                                                                                                                      aaCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCCATGCATGCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCggg                              <  1:2175682/150‑1 (MQ=255)

                                                                                                                      aaCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCggg                              <  1:1133721/150‑1 (MQ=255)

                                                                                                                      aaCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCggg                              >  1:1178322/1‑150 (MQ=255)

                                                                                                                       aCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCACTGCATTCCAGATGATCCGGCGCGGCGAGGTCGAGCTGGCCATTGCCgggg                             >  1:67951/1‑150 (MQ=255)

                                                                                                                       aCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgggg                             <  2:111135/150‑1 (MQ=255)

                                                                                                                       aCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTACCAAGCCATAGGCAGTTTATGCGAGGGAATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgggg                             >  1:2272748/1‑150 (MQ=255)

                                                                                                                        cGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTcg                                                          <  2:2263546/120‑1 (MQ=255)

                                                                                                                        cGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTcg                                                          >  1:2263546/1‑120 (MQ=255)

                                                                                                                        cGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCggggg                            <  1:1911983/150‑1 (MQ=255)

                                                                                                                         gACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCGATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGgc                           >  1:281965/1‑150 (MQ=255)

                                                                                                                         gACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGgc                           >  2:2199307/1‑150 (MQ=255)

                                                                                                                           ccGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGgcgc                         <  2:416422/150‑1 (MQ=255)

                                                                                                                            cGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGcc                        >  1:699080/1‑150 (MQ=255)

                                                                                                                              ccTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGa                      <  1:2169215/150‑1 (MQ=255)

                                                                                                                               cTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGAt                     >  2:1239406/1‑150 (MQ=255)

                                                                                                                                ttGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTAAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGANGATCCGNCGCGGCGAGGTCNAGCTGGCCATTGCCGGGGGCGCCGAtt                    <  1:1960975/150‑1 (MQ=255)

                                                                                                                                  gcgcGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCg                  <  2:982793/150‑1 (MQ=255)

                                                                                                                                    gcgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGt                <  1:890754/150‑1 (MQ=255)

                                                                                                                                      gggTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGc              <  1:1084268/150‑1 (MQ=255)

                                                                                                                                        ttcactGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTc                                                >  1:602550/6‑114 (MQ=255)

                                                                                                                                        ttcactGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTc                                                <  2:602550/109‑1 (MQ=255)

                                                                                                                                          gATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAg          <  2:802849/150‑1 (MQ=255)

                                                                                                                                            ctGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                      <  2:757754/87‑1 (MQ=255)

                                                                                                                                            ctGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                      >  1:757754/2‑88 (MQ=255)

                                                                                                                                              gCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTg      <  1:1497594/150‑1 (MQ=255)

                                                                                                                                                cGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGAtt                    <  1:27104/134‑1 (MQ=255)

                                                                                                                                                cGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGAtt                    >  2:27104/1‑134 (MQ=255)

                                                                                                                                                  aTGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGACCATCCGTCACGGCGAAGTCGACCTGGCGATTGACGAGGGCGCCGATTCGGTGCTTAGCCTGGaca  >  2:437692/1‑150 (MQ=255)

TCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACA  >  NC_002947/3165950‑3166245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`