Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,189,321 G→C 9.5% L458V (CTG→GTG)  PP_2799 ← class III aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,189,3210GC 9.5% 153.8 / 8.8 53L458V (CTG→GTG) PP_2799class III aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (24/24);  new base C (2/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.63e-01

ACACTTGGGCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATC  >  NC_002947/3189172‑3189454
                                                                                                                                                     |                                                                                                                                     
acacTTGGGCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCAcagcag                                                                                                                                       <  2:1734556/150‑1 (MQ=255)
    ttGGGCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGc                                                                                                                                   >  1:1335307/1‑150 (MQ=255)
     ctggCCGTGATGGTGCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACTCACGGTCAAACCCGCATGATTGTTGTTTTACGTGAGTAGCCAGCAGATGCAGCCTTTGCTCACAGCAGGTGCt                                                                                                                                  <  1:1154425/148‑1 (MQ=255)
       ggCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCtgt                                                                                                                                <  1:1191041/150‑1 (MQ=255)
          cATGATGGTTCTCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAg                                                                                                                             >  2:1678202/1‑150 (MQ=255)
          cATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCAcagcag                                                                                                                                       >  1:1692859/1‑140 (MQ=255)
          cATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGAg                                                                                                                             >  1:2170033/1‑150 (MQ=255)
          cATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTCTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCAcagcag                                                                                                                                       <  2:1692859/140‑1 (MQ=255)
             gatgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAgcgc                                                                                                                          <  2:1217904/150‑1 (MQ=255)
              atgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGAgcaaa                                                                                                                         <  2:2170033/150‑4 (MQ=255)
              atgGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCACCTGCTGTGAgcaaa                                                                                                                         <  2:1718290/150‑4 (MQ=255)
                    cccATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAAt                                                                                                                   <  1:863065/150‑1 (MQ=255)
                       aTGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGcc                                                                                                                       <  2:1567211/143‑1 (MQ=255)
                       aTGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGcc                                                                                                                       >  1:1567211/1‑143 (MQ=255)
                             ccGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGt                                                                                                          >  1:445100/1‑150 (MQ=255)
                                   ccGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCATTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTcagcca                                                                                                    >  2:1570146/1‑150 (MQ=255)
                                    cGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTcagccag                                                                                                   >  2:750395/1‑150 (MQ=255)
                                     gATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTcagccagc                                                                                                  <  2:2130386/150‑1 (MQ=255)
                                             aaaCGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGccc                                                                                                                      >  1:214958/1‑122 (MQ=255)
                                             aaaCGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGccc                                                                                                                      <  2:214958/122‑1 (MQ=255)
                                                 gTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAg                                                                                      >  1:1735034/1‑150 (MQ=255)
                                                  tCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCTGATGCAGCCTTTGCTCACAGCAGGTGCtgt                                                                                                                                <  2:2135197/107‑1 (MQ=255)
                                                  tCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCtgt                                                                                                                                >  1:2135197/1‑107 (MQ=255)
                                                  tCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAgg                                                                                     <  1:1678202/150‑1 (MQ=255)
                                                          gAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAg                                                                             >  1:1329588/1‑150 (MQ=255)
                                                          gAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAg                                                                             >  2:994052/1‑150 (MQ=255)
                                                           aaGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGaa                                                                                       >  2:999861/1‑139 (MQ=255)
                                                           aaGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGaa                                                                                       <  1:999861/139‑1 (MQ=255)
                                                             gCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATc                                                                          <  1:2251881/150‑1 (MQ=255)
                                                             gCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATc                                                                          <  2:2129023/150‑1 (MQ=255)
                                                                 tcatcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCt                                                                      >  1:895156/1‑150 (MQ=255)
                                                                  catcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTc                                                                     <  2:1335307/150‑1 (MQ=255)
                                                                   atcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCg                                                                    >  2:1184277/1‑150 (MQ=255)
                                                                    tcaCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGa                                                                   <  2:1735034/150‑1 (MQ=255)
                                                                      aCTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCAGCTCGAAGGCGCTGAAGATCGCCTCGata                                                                 >  1:920683/1‑150 (MQ=255)
                                                                           gtTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTc                                                            <  1:750395/150‑1 (MQ=255)
                                                                              cacgcacgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATc                                                                                                                  >  2:292162/1‑93 (MQ=255)
                                                                              cacgcacgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATc                                                                                                                  <  1:292162/93‑1 (MQ=255)
                                                                               acgcacgcTCACACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTgg                                                        >  2:805235/1‑150 (MQ=255)
                                                                                  cacgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGggtg                                                     <  1:824500/150‑1 (MQ=255)
                                                                                   acgGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGggtgg                                                    <  2:2237561/150‑1 (MQ=255)
                                                                                     ggTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTa                                                  >  1:952572/1‑150 (MQ=255)
                                                                                         aaaCCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCa                                              >  2:2114523/1‑150 (MQ=255)
                                                                                         aaaCCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCACAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCa                                              <  2:928815/150‑1 (MQ=255)
                                                                                              cTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTAACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACgga                                         <  2:2026809/150‑1 (MQ=255)
                                                                                                        ttgttTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAgg                               >  1:1079729/1‑150 (MQ=255)
                                                                                                        ttgttTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAgg                               >  2:88223/1‑150 (MQ=255)
                                                                                                           ttttACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCAGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGa                            <  2:920683/150‑1 (MQ=255)
                                                                                                             ttACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATc                          <  1:1570146/150‑1 (MQ=255)
                                                                                                                            tCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTgg                                                        <  1:421130/105‑1 (MQ=255)
                                                                                                                            tCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTgg                                                        >  2:421130/1‑105 (MQ=255)
                                                                                                                                  gCAGCCTTGGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATg     >  2:2111352/1‑150 (MQ=255)
                                                                                                                                    aGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGAt   >  2:1163377/1‑150 (MQ=255)
                                                                                                                                     gCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATc  <  2:1329588/150‑1 (MQ=255)
                                                                                                                                            cTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGcc                                                                       <  2:619996/74‑1 (MQ=39)
                                                                                                                                            cTCACAGCACCTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGcc                                                                       >  1:619996/1‑74 (MQ=39)
                                                                                                                                                 agcagGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACggagga                                      >  1:983280/1‑102 (MQ=255)
                                                                                                                                                 agcagGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACggagga                                      <  2:983280/102‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                     
ACACTTGGGCCATGATGGTTCCCATGGCACCGAGCCCGATCACGGAAACGTCGAACTCGAAGCTTTCATCACTGTGTTCACGCACGGTCAAACCCTCATGATTGTTGTTTTACGTGAGTAGCCATCAGATGCAGCCTTTGCTCACAGCAGGTGCTGTGAGCGCGCCCAATCCGTCGCGTCAGCCAGCGTCCGCTCGAAGGCGCTGAAGATCGCCTCGATATCGTCCTGGGTGGTAATCAACGGAGGACAGAAGGCGATCGTGTCGTAGATGGCGCGAATGATC  >  NC_002947/3189172‑3189454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑