Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,252,535 G→A 8.0% intergenic (+22/+221) ureG → / ← PP_2851 urease accessory protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,252,5350GA 8.0% 242.4 / 9.2 88intergenic (+22/+221)ureG/PP_2851urease accessory protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (48/32);  new base A (5/2);  total (53/35)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

TGGAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTA  >  NC_002947/3252386‑3252684
                                                                                                                                                     |                                                                                                                                                     
tGGAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCggg                                                                                                                                                       <  2:1214791/150‑1 (MQ=255)
 ggAGCGCGACACCCAGCGCATGCGGCCGAACAGGCACTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGc                                                                                                                                                      >  1:2200389/1‑150 (MQ=255)
        gACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGCGCTAGGCTGACAGATCGAGGTCTCTGTGCGGACATGCCCg                                                                                                                                               >  1:1778696/1‑150 (MQ=255)
          cacCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGca                                                                                                                                             <  1:2092076/150‑1 (MQ=255)
            cccAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGcaca                                                                                                                                           <  1:1096310/150‑1 (MQ=255)
             ccAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACag                                                                                                                                          >  2:1614163/1‑150 (MQ=255)
              cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACaga                                                                                                                                         >  1:1553625/1‑150 (MQ=255)
              cAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACaga                                                                                                                                         <  1:1614163/150‑1 (MQ=255)
               aGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACagag                                                                                                                                        >  1:1886110/1‑150 (MQ=255)
                  gcATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGAcc                                                                                                                                     >  1:911004/1‑150 (MQ=255)
                    aTGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCaa                                                                                                                                   >  2:1419877/1‑150 (MQ=255)
                           cGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGtg                                                                                                                            <  1:1551166/150‑1 (MQ=255)
                            gCAGCGGCCCTAGACCTTCAGCAAGCTGAAGAAGGGCGAGGGGTTGCAGGCCGTTATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGCCTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGtgg                                                                                                                           <  1:632120/150‑1 (MQ=255)
                             cAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGtggc                                                                                                                          >  2:1136904/1‑150 (MQ=255)
                               gCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGTCTCTGTGCGGACATGCCCGCACaga                                                                                                                                         >  2:340554/1‑133 (MQ=255)
                               gCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGTCTCTGTGCGGACATGCCCGCACaga                                                                                                                                         <  1:340554/133‑1 (MQ=255)
                                 ggCCCTGGACCTTCAGCCACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGAATTTATAGTCCAGCGAGGGATGATGGGGGTGGGACACTAACCAATCGATGGCTCTGTGCGGGCCTGTCAGCACAGAGACCAACGCGGtagccgcc                                                                                                                      >  2:550270/1‑146 (MQ=255)
                                           cTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCGGAAGAGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTg                                                                                                            >  1:1778340/1‑150 (MQ=255)
                                           cTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTg                                                                                                            >  1:1841199/1‑150 (MQ=255)
                                           cTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTg                                                                                                            >  1:731238/1‑150 (MQ=255)
                                            ttCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGc                                                                                                           <  2:1719162/150‑1 (MQ=255)
                                                   aCCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGtt                                                                                                    >  1:445554/1‑150 (MQ=255)
                                                    ccTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTc                                                                                                   <  1:1747483/150‑1 (MQ=255)
                                                    ccTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTc                                                                                                   >  1:1868009/1‑150 (MQ=255)
                                                      tGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTACGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTcgc                                                                                                 >  1:2008451/1‑150 (MQ=255)
                                                       gaagaaGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTcgcg                                                                                                >  1:2320775/1‑150 (MQ=255)
                                                        aagaagGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCgg                                                                                               <  2:1991564/150‑1 (MQ=255)
                                                         agaagGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCggg                                                                                              >  1:641045/1‑150 (MQ=255)
                                                         agaagGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCggg                                                                                              <  1:2174395/150‑1 (MQ=255)
                                                             gggCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGtttt                                                                                          >  2:1124956/1‑149 (MQ=255)
                                                             gggCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTa                                                                                          >  1:2310476/1‑150 (MQ=255)
                                                               gCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGTCTGGCACCgg                                                                                                                 <  2:2323981/125‑1 (MQ=255)
                                                               gCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCgg                                                                                                                 >  1:2323981/1‑125 (MQ=255)
                                                               gCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTAcc                                                                                        <  2:1688001/150‑1 (MQ=255)
                                                               gCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTAcc                                                                                        >  2:585967/1‑150 (MQ=255)
                                                                 gAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCAGCTCTGCCGGTGTTCGCGGGTTTACCcg                                                                                      >  1:1115748/1‑150 (MQ=255)
                                                                         gCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACg                                                                              <  2:52716/150‑1 (MQ=255)
                                                                           aGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGt                                                                            >  1:965580/1‑150 (MQ=255)
                                                                            ggCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGTTa                                                                           <  2:1841199/150‑1 (MQ=255)
                                                                              ccGTGATCGATTTTATCGTCGAGCGCGTGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGc                                                                         <  2:1886110/150‑1 (MQ=255)
                                                                                          ttATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTTACAGACCGAGGCCTCTGTGCGGGCATGTCCGAACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAAt                                                             <  1:1666841/150‑1 (MQ=255)
                                                                                          ttATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAAt                                                             <  2:1868009/150‑1 (MQ=255)
                                                                                          tgaTCGGCGAGCGAGGTATGGTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAAt                                                             <  2:948136/148‑1 (MQ=255)
                                                                                             tcgtcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGaa                                                          <  1:2198892/150‑1 (MQ=255)
                                                                                             tcgtcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGaa                                                          >  1:1070180/1‑150 (MQ=255)
                                                                                               gtcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                               <  1:91219/63‑1 (MQ=255)
                                                                                               gtcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCg                                                                                                                                               >  2:91219/1‑63 (MQ=255)
                                                                                               gtcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGCATACGGTCGCGGCCGCAACAATGAAcg                                                        >  1:401320/1‑150 (MQ=255)
                                                                                                tcgAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAAcgg                                                       >  2:701139/1‑150 (MQ=255)
                                                                                                      gcgGGATGCTGGGGGTGCGAGGCTGCCAGCTCGAAGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTg                                                 <  2:965580/150‑1 (MQ=255)
                                                                                                        gggATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCcgc                                                                                     <  1:1742055/112‑1 (MQ=255)
                                                                                                        gggATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCcgc                                                                                     >  2:1742055/1‑112 (MQ=255)
                                                                                                        gggATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGATGGCTGGGCGAGCGACTGCCGGTGTTCGCGTGTTTACCCGCGAATACGGTAGCGGCCAAACAAATGAACGGCGGGTGGa                                               >  1:735068/1‑150 (MQ=255)
                                                                                                            tGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAAtt                                           <  2:636363/150‑1 (MQ=255)
                                                                                                            tGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAAtt                                           >  2:173980/1‑150 (MQ=255)
                                                                                                               tGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGc                                        >  2:2161682/1‑150 (MQ=255)
                                                                                                               tGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACAGTAACGGCAGCAAAAATGAAAGACCGGTGGATATGGGc                                        >  2:1301133/1‑150 (MQ=38)
                                                                                                                      gCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAgg                                 <  1:585967/150‑1 (MQ=255)
                                                                                                                      gCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAgg                                 <  1:1419877/150‑1 (MQ=255)
                                                                                                                        gAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCgg                                                                                               >  2:1958402/1‑86 (MQ=255)
                                                                                                                        gAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCgg                                                                                               <  1:1958402/86‑1 (MQ=255)
                                                                                                                        gAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGggc                               >  2:836436/1‑150 (MQ=255)
                                                                                                                             tGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGggccggcc                          >  1:941595/1‑150 (MQ=255)
                                                                                                                              gACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCCCCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCct                         >  2:1485500/1‑150 (MQ=255)
                                                                                                                               aCAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCctc                        >  2:809089/1‑150 (MQ=255)
                                                                                                                                 aGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTAccc                                                                                       >  1:666830/1‑85 (MQ=255)
                                                                                                                                 aGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTAccc                                                                                       <  2:666830/85‑1 (MQ=255)
                                                                                                                                 aGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCACCACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCtt                      >  2:305051/1‑150 (MQ=255)
                                                                                                                                   aTCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGAGTTTGGCGCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcg                    >  1:1333742/1‑150 (MQ=37)
                                                                                                                                    tCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcgc                   >  2:1906071/1‑150 (MQ=255)
                                                                                                                                    tCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTcgc                   >  1:1490362/1‑150 (MQ=255)
                                                                                                                                      gagaaatctGTGCGGTTGTGGCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCgg                 <  2:1333742/144‑1 (MQ=37)
                                                                                                                                      gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCggg                                                                                              >  1:205234/1‑73 (MQ=255)
                                                                                                                                      gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCggg                                                                                              <  2:205234/73‑1 (MQ=255)
                                                                                                                                      gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCgg                 >  1:620146/1‑150 (MQ=255)
                                                                                                                                      gAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCgg                 <  2:73452/150‑1 (MQ=255)
                                                                                                                                         gACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTAccc                                                                                       >  1:547966/1‑77 (MQ=255)
                                                                                                                                         gACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTAccc                                                                                       <  2:547966/77‑1 (MQ=255)
                                                                                                                                           ctctGTGCGGGCATGTCCGCACAGCCATCTCCGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaaa            >  1:1229162/1‑150 (MQ=37)
                                                                                                                                           ctctGTGCGGACATGCCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCACAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaaa            <  2:1236840/150‑1 (MQ=255)
                                                                                                                                           ctctGTGCGGACATGCCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCACAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTaaa            >  1:1236840/1‑150 (MQ=255)
                                                                                                                                            tctGTGCGGACATGCCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAc           >  2:1103166/1‑150 (MQ=255)
                                                                                                                                            tctGTGCGGACATGCCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAc           >  1:1927316/1‑150 (MQ=255)
                                                                                                                                            gctGTTCGTGCAGGGCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTAGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAc           >  2:1162008/2‑150 (MQ=37)
                                                                                                                                              tgtgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAccc         >  2:1268648/1‑150 (MQ=255)
                                                                                                                                              tgtgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAAccc         >  1:2053498/1‑150 (MQ=255)
                                                                                                                                                ttcGGGCATGTCCGCACAGAGACGAACTTGTTGGCTGGCACCGCCTCTGCCCGTGTCGGCGGTTTTACGCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGc       <  2:731238/148‑1 (MQ=21)
                                                                                                                                                tgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTGGCGGGTacccttgc       <  2:33087/150‑8 (MQ=255)
                                                                                                                                                tgCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGc       <  1:1136904/150‑1 (MQ=255)
                                                                                                                                                   gggCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGTGTAAACCCTCTcc    <  2:735068/150‑1 (MQ=255)
                                                                                                                                                   gggCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc    <  2:1070180/150‑1 (MQ=255)
                                                                                                                                                   gggCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc    >  1:1624457/1‑150 (MQ=255)
                                                                                                                                                     gCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAGTGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTa  >  1:135217/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGAGCGCGACACCCAGCGCATGCGGCCGCAGCGGCCCTGGACCTTCAGCAACCTGAAGAAGGGCGAGGGGTTGCAGGCCGTGATCGATTTTATCGTCGAGCGCGGGATGCTGGGGGTGCGAGGCTGACAGATCGAGGACTCTGTGCGGGCATGTCCGCACAGAGACCAACGCGGTGGCTGGCACCGGCTCTGCCGGTGTTCGCGGGTTTACCCGCGAATACGGTAGCGGCCGCAACAATGAACGGCGGGTGGAAATTGGCCAGCAGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTA  >  NC_002947/3252386‑3252684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑