Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,313,871 T→G 15.1% intergenic (+33/‑126) hemB → / → proP 5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,313,8710TG15.1% 140.1 / 21.3 66intergenic (+33/‑126)hemB/proP5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease
Reads supporting (aligned to +/- strand):  ref base T (27/29);  new base G (3/7);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CCATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAAGAAAAGCG  >  NC_002947/3313729‑3314018
                                                                                                                                              |                                                                                                                                                   
ccATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCgg                                                                                                                                              <  2:1583514/150‑1 (MQ=255)
  aTCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCt                                                                                                                                            >  2:1893867/1‑150 (MQ=255)
  aTCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCt                                                                                                                                            >  1:1633273/1‑150 (MQ=255)
  aTCGACGAAGACCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTg                                                                                                                                                    >  2:2113024/1‑142 (MQ=255)
  aTCGACGAAGACCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTg                                                                                                                                                    <  1:2113024/142‑1 (MQ=255)
   tCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCtt                                                                                                                                           <  1:445724/150‑1 (MQ=255)
   tCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGCtt                                                                                                                                           <  1:738941/150‑1 (MQ=255)
    cgacgaAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGCttt                                                                                                                                          <  2:43514/150‑1 (MQ=255)
    cgacgaAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCCGCAATGGCAGCGGCttt                                                                                                                                          <  2:1196459/150‑1 (MQ=255)
     gacgaAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGCtttc                                                                                                                                         <  2:1255226/150‑2 (MQ=255)
      acgaAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTc                                                                                                                                        <  1:122544/150‑1 (MQ=255)
       cgaAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCt                                                                                                                                       <  1:224251/150‑1 (MQ=255)
        gaAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCtt                                                                                                                                      >  2:1798070/1‑150 (MQ=255)
         aaGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCttt                                                                                                                                     <  2:2061224/150‑1 (MQ=255)
         aaGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCttt                                                                                                                                     <  1:1233421/150‑1 (MQ=255)
            gCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTtgtg                                                                                                                                  >  2:1369883/1‑150 (MQ=255)
                    gtgCGCGAACGCATTGGCGCAATCCAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCCTTGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGttc                                                                                                                          <  1:2089156/150‑2 (MQ=255)
                     tgCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTgg                                                                                                                         <  2:1633273/150‑1 (MQ=255)
                            aaGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGc                                                                                                                  >  2:1368137/1‑150 (MQ=255)
                             aGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGc                                                                                                                                             <  1:2237856/122‑1 (MQ=255)
                             aGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCAATGGCAGCGGc                                                                                                                                             >  2:2237856/1‑122 (MQ=255)
                                 ttGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCaa                                                                                                             >  2:75761/1‑150 (MQ=255)
                                    gcgcAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGggt                                                                                                                       <  1:1900183/137‑1 (MQ=255)
                                    gcgcAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGggt                                                                                                                       >  2:1900183/1‑137 (MQ=255)
                                    gcgcAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACg                                                                                                          >  2:1857216/1‑150 (MQ=255)
                                    gcgcAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACg                                                                                                          >  2:1429823/1‑150 (MQ=255)
                                      gcAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGca                                                                                                        <  2:268357/150‑1 (MQ=255)
                                       cAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGcac                                                                                                       <  1:75761/150‑1 (MQ=255)
                                        aaTCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGcaca                                                                                                      >  1:2318892/1‑150 (MQ=255)
                                              gCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACaaaaaaa                                                                                                <  1:2306372/150‑1 (MQ=255)
                                                gTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGtt                                                                                                                           <  1:77900/121‑1 (MQ=255)
                                                gTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGtt                                                                                                                           >  2:77900/1‑121 (MQ=255)
                                                  gCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGt                                                                                            <  1:1429823/150‑1 (MQ=255)
                                                             ccTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAat                                                                                 >  1:356105/1‑150 (MQ=255)
                                                                   ccTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCa                                                                                                                                                  >  1:909251/1‑79 (MQ=255)
                                                                   ccTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCa                                                                                                                                                  <  2:909251/79‑1 (MQ=255)
                                                                       aCCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTgg                                                                                                                         >  2:2230740/1‑100 (MQ=255)
                                                                       aCCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTgg                                                                                                                         <  1:2230740/100‑1 (MQ=255)
                                                                         cTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTcc                                                                     >  1:1076528/1‑150 (MQ=255)
                                                                           aCTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCtg                                                                   >  2:343259/1‑150 (MQ=255)
                                                                           aCTACGCAATAGAACTGGGGCGAGAAGGTATATGAAGCCAGGCATTGGTTGTGAAAGCTGCTGCCAATGGAGAGGCATTGCTTTGTGCACCGTTGGGTGGTGCCGAAAAAGTACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCtg                                                                   <  2:697432/150‑1 (MQ=255)
                                                                             ttCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCtgtt                                                                 <  2:1546297/150‑1 (MQ=255)
                                                                                  cATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATc                                                            >  2:1850026/1‑150 (MQ=255)
                                                                                      gACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGAt                                                        <  1:1368137/150‑1 (MQ=255)
                                                                                            gcgcgcGAAGGTATCTGAAGCCAGGCCTTGGTCATGCACCCCGCAGCGCTTCCAGCGGCTTTTCTTTGTGCGCCGGTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTa                                                  >  1:406378/1‑150 (MQ=255)
                                                                                              gcgcgAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTaga                                                <  1:1893867/150‑1 (MQ=255)
                                                                                              gcgcgAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTaga                                                <  1:1857216/150‑1 (MQ=255)
                                                                                                 cgAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCa                                             <  2:356105/150‑1 (MQ=255)
                                                                                                       tATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCGTTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATc                                       >  1:1891802/1‑150 (MQ=255)
                                                                                                         tCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGa                                     >  2:2143293/1‑150 (MQ=255)
                                                                                                          cTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCAC‑AAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACa                                   <  1:1369883/150‑1 (MQ=255)
                                                                                                           tGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAACCGCTCAACCATCCAGTCCGACCGCGATTATGTAGTGGCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACa                                   <  1:1798070/150‑1 (MQ=255)
                                                                                                           tGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACa                                   <  2:1076528/150‑1 (MQ=255)
                                                                                                                       ttGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAAAAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTa                       >  2:74198/1‑150 (MQ=255)
                                                                                                                             aTGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAAc                 <  1:1850026/150‑1 (MQ=255)
                                                                                                                              tGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAAcc                >  2:2232585/1‑150 (MQ=255)
                                                                                                                                aaaGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTc              <  2:406378/150‑1 (MQ=255)
                                                                                                                                  aGCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTa                                                  >  2:1711397/1‑112 (MQ=255)
                                                                                                                                  aGCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTa                                                  <  1:1711397/112‑1 (MQ=255)
                                                                                                                                   gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGa                                                                                   <  1:707497/78‑1 (MQ=255)
                                                                                                                                   gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGa                                                                                   >  2:707497/1‑78 (MQ=39)
                                                                                                                                    ccGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCaa          >  2:1793102/1‑150 (MQ=255)
                                                                                                                                          ccATTGCAGCGGCTTTTCTTTGTGCGCCGTTGTGTGGTGCCGCAAGCGCCCACAAAGGCAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTCGAGCATTCATCGACCGGGATCCGGGTATGGCACCTCGCAAGAAAAg    >  1:669665/1‑150 (MQ=255)
                                                                                                                                            aTTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCt               >  1:1880247/1‑137 (MQ=255)
                                                                                                                                            aTTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCt               <  2:1880247/137‑1 (MQ=255)
                                                                                                                                            aTTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAAGAAAAGCg  >  1:641066/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CCATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTCGCAAGAAAAGCG  >  NC_002947/3313729‑3314018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑