Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,528,036 A→G 5.4% S402G (AGC→GGC)  imuB → DNA repair DNA linked protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,528,0360AG 5.4% 289.2 / 4.1 94S402G (AGC→GGC) imuBDNA repair DNA linked protein
Reads supporting (aligned to +/- strand):  ref base A (34/54);  new base G (3/2);  total (37/57)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

AACCCTGGGCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGACGGCCTG  >  NC_002947/3527887‑3528185
                                                                                                                                                     |                                                                                                                                                     
aaCCCTGGGCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCa                                                                                                                                                       >  1:103528/1‑150 (MQ=255)
  cccTGGGCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgc                                                                                                                                                     >  1:583424/1‑150 (MQ=255)
    cTGGGCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCACGGCAACATGCCGGTTGCACCGGGCAgccc                                                                                                                                                   >  1:626093/1‑149 (MQ=255)
    cTGGGCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgcgg                                                                                                                                                   >  1:369988/1‑148 (MQ=255)
     tGGGCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgccgc                                                                                                                                                  <  2:1541848/150‑1 (MQ=255)
      gggCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgccgcc                                                                                                                                                 <  2:1753/150‑1 (MQ=255)
       ggCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGccc                                                                                                                                                <  2:1136737/150‑1 (MQ=255)
          gcAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCggg                                                                                                                                             <  2:909638/150‑1 (MQ=255)
          gcAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCggg                                                                                                                                             <  1:1345419/150‑1 (MQ=255)
            aaTTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGt                                                                                                                                           <  2:270410/150‑1 (MQ=255)
            aaTTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGt                                                                                                                                           <  1:2030324/150‑1 (MQ=255)
             aTTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGGAGCCGCCCGGGGTg                                                                                                                                          >  1:183357/1‑150 (MQ=255)
              ttGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTgg                                                                                                                                         <  2:386645/150‑1 (MQ=255)
              ttGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTgg                                                                                                                                         <  2:242446/150‑1 (MQ=255)
               tGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGc                                                                                                                                        <  2:828991/150‑1 (MQ=255)
                   cgAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCtgtt                                                                                                                                    <  2:44953/150‑1 (MQ=255)
                   cgAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCtgtt                                                                                                                                    <  1:421488/150‑1 (MQ=255)
                      gCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTc                                                                                                                                 <  1:2313403/150‑1 (MQ=255)
                      gCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTc                                                                                                                                 >  1:312160/1‑150 (MQ=255)
                        ggTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCgg                                                                                                                               >  2:645476/1‑150 (MQ=255)
                           tGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCAc                                                                                                                            <  1:1432708/150‑1 (MQ=255)
                              gcgcCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGa                                                                                                                         <  2:2038148/150‑1 (MQ=255)
                                     ccTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCt                                                                                                                  <  1:1146345/150‑1 (MQ=255)
                                       tGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCa                                                                                                                                                       <  1:311824/111‑1 (MQ=255)
                                       tGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCa                                                                                                                                                       >  2:311824/1‑111 (MQ=255)
                                       tGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCgcgcaat                                                                                                                <  2:183357/150‑4 (MQ=255)
                                       tGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTgg                                                                                                                <  1:2040960/150‑1 (MQ=255)
                                        gggggATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCTGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTggg                                                                                                               >  1:2007859/1‑149 (MQ=255)
                                        gggggATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgcc                                                                                                                                                    <  2:1799318/113‑1 (MQ=255)
                                        gggggATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgcc                                                                                                                                                    >  1:1799318/1‑113 (MQ=255)
                                                 ggCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGAcgccgc                                                                                                      <  1:3399/150‑1 (MQ=255)
                                                  gCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGAACCGATCGCGCTGGATGACgccgcc                                                                                                     <  1:600011/150‑1 (MQ=255)
                                                      tCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCTGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTAcc                                                                                                 >  1:2328762/1‑150 (MQ=255)
                                                               ttGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAgg                                                                                        >  1:1252339/1‑150 (MQ=255)
                                                               ttGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAgg                                                                                        <  2:579793/150‑1 (MQ=255)
                                                               ttGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTAGCGTTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTAGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAgg                                                                                        >  2:82651/1‑150 (MQ=255)
                                                                     cAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATg                                                                                  <  1:800798/150‑1 (MQ=255)
                                                                          gccgATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACGGATCGCGCTTGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGaa                                                                             <  1:2157575/150‑1 (MQ=255)
                                                                           ccgATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCtgt                                                                                                                                     >  1:2281706/1‑93 (MQ=255)
                                                                           ccgATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCtgt                                                                                                                                     <  2:2281706/93‑1 (MQ=255)
                                                                              aTCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCa                                                                         <  2:441238/150‑1 (MQ=255)
                                                                                   cGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCTGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGaa                                                                    <  2:2328762/150‑1 (MQ=255)
                                                                                    gCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAAt                                                                   <  1:1378650/150‑1 (MQ=255)
                                                                                      cccGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCa                                                                 <  1:131314/150‑1 (MQ=255)
                                                                                      cccGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGACGCCGCTCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCa                                                                 <  1:735287/150‑1 (MQ=255)
                                                                                       ccGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAg                                                                <  2:2089765/150‑1 (MQ=255)
                                                                                       ccGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAg                                                                >  1:993082/1‑150 (MQ=255)
                                                                                       ccGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAg                                                                <  1:456292/150‑1 (MQ=255)
                                                                                             gcgcCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTtggt                                                          <  1:185464/150‑1 (MQ=255)
                                                                                                  tGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgccgc                                                                                                                                                  >  1:288092/1‑57 (MQ=255)
                                                                                                  tGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAgccgc                                                                                                                                                  <  2:288092/57‑1 (MQ=255)
                                                                                                    gCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCTGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGTCCCGTCCCAGTCGCGGGTCTATGCCGAACGCATCGACTCCGGTTGGTGGGATgg                                                   <  2:1248640/150‑1 (MQ=255)
                                                                                                      aTTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAAc                                                                            <  1:214292/123‑1 (MQ=255)
                                                                                                      aTTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAAc                                                                            >  2:214292/1‑123 (MQ=255)
                                                                                                      aTTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATggcg                                                 <  1:1271326/150‑1 (MQ=255)
                                                                                                           gCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAAc                                                                            <  1:769255/118‑1 (MQ=255)
                                                                                                           gCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAAc                                                                            >  2:769255/1‑118 (MQ=255)
                                                                                                           gCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGAt                                            <  2:1317439/150‑1 (MQ=255)
                                                                                                           gCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGAt                                            <  2:103528/150‑1 (MQ=255)
                                                                                                            cGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGAACCGGGCAGCCGGCCGGGGTGGCTGTTGTCGGCACCTTTCGAGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGAtg                                           >  1:1693059/1‑150 (MQ=255)
                                                                                                             ggAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCAATGCCGAACGCCTCTAATCAGGTTGGTGGGAGGGCGGCGAtgt                                          <  2:1436916/150‑1 (MQ=255)
                                                                                                               aGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGc                                        <  2:1174512/150‑1 (MQ=255)
                                                                                                                gCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAg                                                                                         <  2:69746/100‑1 (MQ=255)
                                                                                                                gCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAg                                                                                         >  1:69746/1‑100 (MQ=255)
                                                                                                                   gggTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTcg                                    >  2:480033/1‑150 (MQ=255)
                                                                                                                   gggTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTcg                                    <  1:480033/150‑1 (MQ=255)
                                                                                                                    ggTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGt                                                              >  2:1056728/1‑123 (MQ=255)
                                                                                                                    ggTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGt                                                              <  1:1056728/123‑1 (MQ=255)
                                                                                                                    ggTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATAGTATCATGTTGGTGGGATGGCGGCGATGTGCGTcgc                                   <  1:450662/150‑1 (MQ=255)
                                                                                                                        cccAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGact                               >  2:1395234/1‑150 (MQ=255)
                                                                                                                         ccAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGacta                              >  2:394734/1‑150 (MQ=255)
                                                                                                                           aaGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGactact                            >  2:665469/1‑150 (MQ=255)
                                                                                                                             ggCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACtactac                          >  2:1732162/1‑150 (MQ=255)
                                                                                                                              gCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTc                                     <  2:773291/138‑1 (MQ=255)
                                                                                                                              gCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTc                                     >  1:773291/1‑138 (MQ=255)
                                                                                                                                   ctGCCCGGTGCAACCGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGc                                        <  1:1937000/129‑1 (MQ=255)
                                                                                                                                       cGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGaaa                <  1:394734/150‑1 (MQ=255)
                                                                                                                                        ggTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAAc               <  2:312160/150‑1 (MQ=255)
                                                                                                                                        ggTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTAGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAAc               <  2:1252339/150‑1 (MQ=255)
                                                                                                                                         gTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCa                                                                                          <  1:1048213/74‑1 (MQ=255)
                                                                                                                                         gTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCa                                                                                          >  2:1048213/1‑74 (MQ=255)
                                                                                                                                          ttGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAcccc             <  2:583424/150‑1 (MQ=255)
                                                                                                                                          ccaccccGGGCGGCTGCCCGGTGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAAccc             >  1:792357/6‑150 (MQ=255)
                                                                                                                                             ccccGGGCGGCTGCCCGGTGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTcgc                                   <  2:2241033/123‑1 (MQ=255)
                                                                                                                                             ccccGGGCGGCTGCCCGGTGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTcgc                                   >  1:2241033/3‑125 (MQ=255)
                                                                                                                                              cccGGGCGGCTGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTcgc                                   <  1:650256/123‑1 (MQ=255)
                                                                                                                                              cccGGGCGGCTGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTcgc                                   >  2:650256/2‑124 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATgg                                                   >  2:2298603/1‑108 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATgg                                                   <  1:2298603/108‑1 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATg                                                    >  2:2298563/1‑107 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATg                                                    <  1:2298563/107‑1 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGa         >  2:2103372/1‑150 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGa         >  2:1293890/1‑150 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTAGAGACTACTACCGCATCGAAACCCGTGa         >  2:54784/1‑150 (MQ=255)
                                                                                                                                              aCCGGGCAGCCGCCCGGGGTGGCTGTTGCCGGAACAGCTTGGGATAGTGGCCTTCCGGAAATAGGTGAAAAGCAAGGCCGAACGCATCGAATCAGGTTGGTTGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGa         >  1:634107/1‑150 (MQ=255)
                                                                                                                                                 gggCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGACgg      >  1:1307493/1‑150 (MQ=255)
                                                                                                                                                  ggCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGACGGc     >  1:1683066/1‑150 (MQ=255)
                                                                                                                                                     aGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGACGGCCTg  >  1:198740/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCCTGGGCGCAATTGCGCGAGCGGTTGCGCGCCCGCCTGGGGGATGAGGCCGTCAAGGGGCTTGGCGCAGCCGCCGATCACCGCCCCGAATGCGCCTGGCATTTGGCGGAGCAGGGTGCCCAAGGCAACATGCCGGTTGCACCGGGCAGCCGCCCGGGGTGGCTGTTGTCGGCACCGATCGCGCTGGATGACGCCGCCTACCAGGTGCAGGGCTATGCCGAACGCATCGAATCAGGTTGGTGGGATGGCGGCGATGTGCGTCGCGACTACTACCGCATCGAAACCCGTGACGGCCTG  >  NC_002947/3527887‑3528185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑