Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,063,045 C→G 5.3% E446D (GAG→GAC) ‡ PP_3581 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,063,0450CG 5.3% 290.4 / 7.0 94E446D (GAG→GAC) ‡PP_3581membrane protein
Reads supporting (aligned to +/- strand):  ref base C (47/42);  new base G (3/2);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

GCCCGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTGG  >  NC_002947/4062897‑4063192
                                                                                                                                                    |                                                                                                                                                   
gCCCGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTAGTAATGCAAGCGGAACTGGCgctgct                                                                                                                                                    >  2:956620/1‑150 (MQ=255)
 cccGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTc                                                                                                                                                   >  1:559014/1‑150 (MQ=255)
  ccGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTcc                                                                                                                                                  <  1:596129/150‑1 (MQ=255)
   cGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTc                                                                                                                                                   >  1:116276/1‑148 (MQ=255)
   cGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTc                                                                                                                                                   <  2:116276/148‑1 (MQ=255)
     gTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAgc                                                                                                                                               >  2:1091449/1‑150 (MQ=255)
        ttttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTTGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGcc                                                                                                                                            >  2:1188918/1‑150 (MQ=255)
        ttttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGcc                                                                                                                                            <  2:1499873/150‑1 (MQ=255)
        ttttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGATGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGcc                                                                                                                                            >  1:1371243/1‑150 (MQ=255)
          ttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCACCaacaaaag                                                                                                                                          <  2:1629354/150‑8 (MQ=255)
            ccGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGGTGCGGGTGCTCCCAGCGTAGCAACGCTGCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGgc                                                                                                                                        <  2:1017530/150‑1 (MQ=255)
              gCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGgcgc                                                                                                                                      >  2:947592/1‑150 (MQ=255)
                      gATGAAGCGGTCAGGCAGCAGCAGGCCCTGATGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTg                                                                                                                              <  2:1371243/150‑1 (MQ=255)
                          aaGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTgcagc                                                                                                                          >  2:663332/1‑150 (MQ=255)
                           aGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTgcagca                                                                                                                         <  1:1091449/150‑1 (MQ=255)
                               gTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGTGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTg                                                                                                                     >  1:539750/1‑150 (MQ=255)
                                  aGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGTGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCaa                                                                                                                  <  2:539750/150‑1 (MQ=255)
                                    gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTg                                                                                                                              >  1:774375/1‑136 (MQ=255)
                                    gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTg                                                                                                                              <  2:774375/136‑1 (MQ=255)
                                    gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAAct                                                                                                                >  2:738954/1‑150 (MQ=255)
                                    gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACAGATCTGCGGGCAGGCCTAGGCGTCGAACTTGTGCTGGTAATGCAAGCGACAGCGGCACTGCTCCAGCGCCAGGCGCAAGTCCTGCGGCAGCTGCAAct                                                                                                                >  2:1398945/1‑150 (MQ=255)
                                      agcagcagGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAActgc                                                                                                              >  1:2066514/1‑150 (MQ=255)
                                       gcagcagGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAActgct                                                                                                             >  1:391883/1‑150 (MQ=255)
                                        cagcagGCCCTGCTGCCGGTGCTCCGAGCGCAGCAGCGCATCGTCGCGGATCGGTTCGCAGGCCTTGTCCTCGAACTTTGGCTGTCCAGGCAAGCATAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAActgctg                                                                                                            <  2:391883/150‑1 (MQ=255)
                                            agGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGgcgc                                                                                                        >  2:2000931/1‑150 (MQ=255)
                                             ggCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                          <  2:481732/115‑1 (MQ=255)
                                             ggCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                          >  1:481732/1‑115 (MQ=255)
                                               gccTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCa                                                                                                     >  2:2032529/2‑150 (MQ=255)
                                               cccTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTAGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCa                                                                                                     <  1:956620/150‑1 (MQ=255)
                                                  tgctgcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTg                                                                                                  >  1:2168046/1‑150 (MQ=255)
                                                   gctgcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCCGgcgcgctggtt                                                                                                 <  1:1273686/150‑6 (MQ=255)
                                                     tgcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTg                                                                                               <  1:2032529/150‑1 (MQ=255)
                                                      gcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGt                                                                                              <  2:1506573/150‑1 (MQ=255)
                                                           tGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                         <  1:1325967/150‑1 (MQ=255)
                                                           tGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                         <  1:455542/150‑1 (MQ=255)
                                                             cacccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTTTTGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCgg                                                                                       <  1:735481/148‑2 (MQ=255)
                                                               cccAGAGCAGCCACGGTTCAGCAACGAAAGGTGGGCAGGCCGGGGTGTCGAACTTTGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGaca                                                                                     <  2:504466/150‑1 (MQ=255)
                                                                ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTg                                                                                                                     <  1:1703073/117‑1 (MQ=255)
                                                                ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTg                                                                                                                     >  2:1703073/1‑117 (MQ=255)
                                                                ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGacac                                                                                    >  2:1457378/1‑150 (MQ=255)
                                                                ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGacac                                                                                    >  1:492881/1‑150 (MQ=255)
                                                                ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGacac                                                                                    >  1:333610/1‑150 (MQ=255)
                                                                  aGCGCCGCAGCGCGTCGGCACGGGTCGGTTGGCAGGCCTGGGCGTCGAACTTTGGCTGGGAATGCAAGCGGAGCTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACAt                                                                                  >  2:89440/1‑150 (MQ=255)
                                                                  aGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACAt                                                                                  <  1:1107937/150‑1 (MQ=255)
                                                                    cgcAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCg                                                                                <  1:663332/150‑1 (MQ=255)
                                                                      cagcaACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCgaa                                                                              <  2:1916605/150‑1 (MQ=255)
                                                                      cagcaACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCgaa                                                                              <  1:2000931/150‑1 (MQ=255)
                                                                       agcaACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCgaag                                                                             >  1:1408424/1‑150 (MQ=255)
                                                                          aaCGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGaagaag                                                                          >  2:1185871/1‑150 (MQ=255)
                                                                            cGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCt                                                                        <  1:442787/150‑1 (MQ=255)
                                                                            cGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCt                                                                        <  2:2168046/150‑1 (MQ=255)
                                                                             gCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTg                                                                       >  2:2053751/1‑150 (MQ=255)
                                                                              cTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                      >  1:427129/1‑150 (MQ=255)
                                                                              cTTCGGCACCGATCGGTTGGCAGGCATGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                      <  1:452827/150‑1 (MQ=255)
                                                                               ttCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                          <  1:2217686/81‑1 (MQ=255)
                                                                               ttCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                          >  2:2217686/1‑81 (MQ=255)
                                                                                   gCACCGATCGGTTGGCAGGCCTGGGCGTCGACATTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAAct                                                                                                                <  1:755072/103‑1 (MQ=255)
                                                                                   gCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAAct                                                                                                                >  2:755072/1‑103 (MQ=255)
                                                                                     aCCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGtt                                                               <  1:1357121/150‑1 (MQ=255)
                                                                                      ccGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTc                                                              <  1:947592/150‑1 (MQ=255)
                                                                                             gTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCgg                                                       >  2:592286/1‑150 (MQ=255)
                                                                                             gTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCgg                                                       >  2:1472753/1‑150 (MQ=255)
                                                                                                   aGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCTGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCttt                                                 >  1:1353561/1‑150 (MQ=255)
                                                                                                   aGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCttt                                                 >  2:152510/1‑150 (MQ=255)
                                                                                                     gCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTg                                               <  2:427129/150‑1 (MQ=255)
                                                                                                         gggCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                         <  1:1083260/104‑1 (MQ=255)
                                                                                                         gggCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                         >  2:1083260/1‑104 (MQ=255)
                                                                                                         gggCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCAt                                           <  2:2066514/150‑1 (MQ=255)
                                                                                                              tCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGAAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAAACGTTCTTGCCGGCGCTTTTGGCATGGTAc                                      >  1:566366/1‑150 (MQ=255)
                                                                                                                gAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACAt                                    >  2:2028499/1‑150 (MQ=255)
                                                                                                                 aaCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATg                                   >  1:2081776/1‑150 (MQ=255)
                                                                                                                 aaCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATg                                   >  2:533396/1‑150 (MQ=255)
                                                                                                                  aCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATgg                                  >  1:2329141/1‑150 (MQ=255)
                                                                                                                  aCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATgg                                  >  1:2215089/1‑150 (MQ=255)
                                                                                                                  aCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATgg                                  <  2:2329141/150‑1 (MQ=255)
                                                                                                                        gCTGGTAATTCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGaca                                                                                     <  1:978026/93‑1 (MQ=255)
                                                                                                                        gCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGaca                                                                                     >  2:978026/1‑93 (MQ=255)
                                                                                                                        gCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGt                            >  2:1416922/1‑150 (MQ=255)
                                                                                                                        gCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCCATGCTCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGt                            <  1:122430/150‑1 (MQ=255)
                                                                                                                         cTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTc                           >  2:1840624/1‑150 (MQ=255)
                                                                                                                            gTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGc                        <  1:2053751/150‑1 (MQ=255)
                                                                                                                             tAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCg                       >  1:1445403/1‑150 (MQ=255)
                                                                                                                                   aaGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACgc                 >  1:1934347/1‑150 (MQ=255)
                                                                                                                                     gCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGcc                                                         >  1:117571/1‑108 (MQ=255)
                                                                                                                                     gCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGcc                                                         <  2:117571/108‑1 (MQ=255)
                                                                                                                                       ggAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACgcagca             <  1:738954/150‑1 (MQ=255)
                                                                                                                                        gAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTc                                                              <  2:340006/100‑1 (MQ=255)
                                                                                                                                        gAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTc                                                              >  1:340006/1‑100 (MQ=255)
                                                                                                                                        gAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGcagcag            >  2:1285859/1‑150 (MQ=255)
                                                                                                                                         aaCTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCagcagc           >  2:675817/1‑150 (MQ=255)
                                                                                                                                           cTGGCGCTGGAGCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTAc                   >  2:372387/1‑140 (MQ=255)
                                                                                                                                            tGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCg        >  1:318193/1‑150 (MQ=255)
                                                                                                                                             ggCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGt       <  1:1457378/150‑1 (MQ=255)
                                                                                                                                              gcgcTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTg      <  1:372318/150‑1 (MQ=255)
                                                                                                                                               cgcTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGc     <  1:1138757/150‑1 (MQ=255)
                                                                                                                                                gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                      <  1:1552989/84‑1 (MQ=255)
                                                                                                                                                gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                      >  2:1552989/1‑84 (MQ=255)
                                                                                                                                                gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTc         <  2:1480034/145‑1 (MQ=255)
                                                                                                                                                gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTc         >  1:1480034/1‑145 (MQ=255)
                                                                                                                                                  tgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTgg  >  1:180251/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCCGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTGG  >  NC_002947/4062897‑4063192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑