BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,063,047	C→G	5.3%	E446Q (GAG→CAG) ‡	PP_3581 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,063,047	0	C	G	5.3%	302.6 / 6.3	95	E446Q (GAG→CAG) ‡	PP_3581	membrane protein
Reads supporting (aligned to +/- strand): ref base C (48/42); new base G (3/2); total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 101 total aligned reads displayed.

CCCGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTGGTCCT  >  NC_002947/4062898‑4063196

cccGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTc                                                                                                                                                       >  1:559014/1‑150 (MQ=255)

 ccGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTcc                                                                                                                                                      <  1:596129/150‑1 (MQ=255)

  cGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTc                                                                                                                                                       >  1:116276/1‑148 (MQ=255)

  cGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTc                                                                                                                                                       <  2:116276/148‑1 (MQ=255)

    gTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAgc                                                                                                                                                   >  2:1091449/1‑150 (MQ=255)

       ttttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTTGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGcc                                                                                                                                                >  2:1188918/1‑150 (MQ=255)

       ttttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGcc                                                                                                                                                <  2:1499873/150‑1 (MQ=255)

       ttttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGATGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGcc                                                                                                                                                >  1:1371243/1‑150 (MQ=255)

         ttCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCACCaacaaaag                                                                                                                                              <  2:1629354/150‑8 (MQ=255)

           ccGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGGTGCGGGTGCTCCCAGCGTAGCAACGCTGCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGgc                                                                                                                                            <  2:1017530/150‑1 (MQ=255)

             gCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGgcgc                                                                                                                                          >  2:947592/1‑150 (MQ=255)

                     gATGAAGCGGTCAGGCAGCAGCAGGCCCTGATGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTg                                                                                                                                  <  2:1371243/150‑1 (MQ=255)

                         aaGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTgcagc                                                                                                                              >  2:663332/1‑150 (MQ=255)

                          aGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTgcagca                                                                                                                             <  1:1091449/150‑1 (MQ=255)

                              gTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGTGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTg                                                                                                                         >  1:539750/1‑150 (MQ=255)

                                 aGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGTGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCaa                                                                                                                      <  2:539750/150‑1 (MQ=255)

                                   gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTg                                                                                                                                  >  1:774375/1‑136 (MQ=255)

                                   gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTg                                                                                                                                  <  2:774375/136‑1 (MQ=255)

                                   gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAAct                                                                                                                    >  2:738954/1‑150 (MQ=255)

                                   gcagcagcaGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACAGATCTGCGGGCAGGCCTAGGCGTCGAACTTGTGCTGGTAATGCAAGCGACAGCGGCACTGCTCCAGCGCCAGGCGCAAGTCCTGCGGCAGCTGCAAct                                                                                                                    >  2:1398945/1‑150 (MQ=255)

                                     agcagcagGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAActgc                                                                                                                  >  1:2066514/1‑150 (MQ=255)

                                      gcagcagGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAActgct                                                                                                                 >  1:391883/1‑150 (MQ=255)

                                       cagcagGCCCTGCTGCCGGTGCTCCGAGCGCAGCAGCGCATCGTCGCGGATCGGTTCGCAGGCCTTGTCCTCGAACTTTGGCTGTCCAGGCAAGCATAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAActgctg                                                                                                                <  2:391883/150‑1 (MQ=255)

                                           agGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGgcgc                                                                                                            >  2:2000931/1‑150 (MQ=255)

                                            ggCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                              >  1:481732/1‑115 (MQ=255)

                                            ggCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                              <  2:481732/115‑1 (MQ=255)

                                              gccTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCa                                                                                                         >  2:2032529/2‑150 (MQ=255)

                                              cccTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTAGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCa                                                                                                         <  1:956620/150‑1 (MQ=255)

                                                 tgctgcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTg                                                                                                      >  1:2168046/1‑150 (MQ=255)

                                                  gctgcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCCGgcgcgctggtt                                                                                                     <  1:1273686/150‑6 (MQ=255)

                                                    tgcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTg                                                                                                   <  1:2032529/150‑1 (MQ=255)

                                                     gcGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGt                                                                                                  <  2:1506573/150‑1 (MQ=255)

                                                          tGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                             <  1:455542/150‑1 (MQ=255)

                                                          tGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                             <  1:1325967/150‑1 (MQ=255)

                                                            cacccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTTTTGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCgg                                                                                           <  1:735481/148‑2 (MQ=255)

                                                              cccAGAGCAGCCACGGTTCAGCAACGAAAGGTGGGCAGGCCGGGGTGTCGAACTTTGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGaca                                                                                         <  2:504466/150‑1 (MQ=255)

                                                               ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTg                                                                                                                         >  2:1703073/1‑117 (MQ=255)

                                                               ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTg                                                                                                                         <  1:1703073/117‑1 (MQ=255)

                                                               ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGacac                                                                                        >  1:492881/1‑150 (MQ=255)

                                                               ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGacac                                                                                        >  2:1457378/1‑150 (MQ=255)

                                                               ccAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGacac                                                                                        >  1:333610/1‑150 (MQ=255)

                                                                 aGCGCCGCAGCGCGTCGGCACGGGTCGGTTGGCAGGCCTGGGCGTCGAACTTTGGCTGGGAATGCAAGCGGAGCTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACAt                                                                                      >  2:89440/1‑150 (MQ=255)

                                                                 aGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACAt                                                                                      <  1:1107937/150‑1 (MQ=255)

                                                                   cgcAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCg                                                                                    <  1:663332/150‑1 (MQ=255)

                                                                     cagcaACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCgaa                                                                                  <  2:1916605/150‑1 (MQ=255)

                                                                     cagcaACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCgaa                                                                                  <  1:2000931/150‑1 (MQ=255)

                                                                      agcaACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCgaag                                                                                 >  1:1408424/1‑150 (MQ=255)

                                                                         aaCGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGaagaag                                                                              >  2:1185871/1‑150 (MQ=255)

                                                                           cGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCt                                                                            <  1:442787/150‑1 (MQ=255)

                                                                           cGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCt                                                                            <  2:2168046/150‑1 (MQ=255)

                                                                            gCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTg                                                                           >  2:2053751/1‑150 (MQ=255)

                                                                             cTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                          >  1:427129/1‑150 (MQ=255)

                                                                             cTTCGGCACCGATCGGTTGGCAGGCATGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                          <  1:452827/150‑1 (MQ=255)

                                                                              ttCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                              <  1:2217686/81‑1 (MQ=255)

                                                                              ttCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGGAGCAGCGCCAg                                                                                                                                              >  2:2217686/1‑81 (MQ=255)

                                                                                  gCACCGATCGGTTGGCAGGCCTGGGCGTCGACATTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAAct                                                                                                                    <  1:755072/103‑1 (MQ=255)

                                                                                  gCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAAct                                                                                                                    >  2:755072/1‑103 (MQ=255)

                                                                                    aCCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGtt                                                                   <  1:1357121/150‑1 (MQ=255)

                                                                                     ccGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTc                                                                  <  1:947592/150‑1 (MQ=255)

                                                                                            gTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCgg                                                           >  2:592286/1‑150 (MQ=255)

                                                                                            gTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCgg                                                           >  2:1472753/1‑150 (MQ=255)

                                                                                                  aGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCTGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCttt                                                     >  1:1353561/1‑150 (MQ=255)

                                                                                                  aGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCttt                                                     >  2:152510/1‑150 (MQ=255)

                                                                                                    gCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTg                                                   <  2:427129/150‑1 (MQ=255)

                                                                                                        gggCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                             <  1:1083260/104‑1 (MQ=255)

                                                                                                        gggCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATc                                                                                             >  2:1083260/1‑104 (MQ=255)

                                                                                                        gggCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCAt                                               <  2:2066514/150‑1 (MQ=255)

                                                                                                             tCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGAAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAAACGTTCTTGCCGGCGCTTTTGGCATGGTAc                                          >  1:566366/1‑150 (MQ=255)

                                                                                                               gAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACAt                                        >  2:2028499/1‑150 (MQ=255)

                                                                                                                aaCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATg                                       >  2:533396/1‑150 (MQ=255)

                                                                                                                aaCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATg                                       >  1:2081776/1‑150 (MQ=255)

                                                                                                                 aCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATgg                                      >  1:2215089/1‑150 (MQ=255)

                                                                                                                 aCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATgg                                      <  2:2329141/150‑1 (MQ=255)

                                                                                                                 aCTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATgg                                      >  1:2329141/1‑150 (MQ=255)

                                                                                                                       gCTGGTAATTCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGaca                                                                                         <  1:978026/93‑1 (MQ=255)

                                                                                                                       gCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGt                                >  2:1416922/1‑150 (MQ=255)

                                                                                                                       gCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCCATGCTCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGt                                <  1:122430/150‑1 (MQ=255)

                                                                                                                        cTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTc                               >  2:1840624/1‑150 (MQ=255)

                                                                                                                           gTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGc                            <  1:2053751/150‑1 (MQ=255)

                                                                                                                            tAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCg                           >  1:1445403/1‑150 (MQ=255)

                                                                                                                                  aaGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACgc                     >  1:1934347/1‑150 (MQ=255)

                                                                                                                                    gCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGcc                                                             >  1:117571/1‑108 (MQ=255)

                                                                                                                                    gCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGcc                                                             <  2:117571/108‑1 (MQ=255)

                                                                                                                                      ggAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACgcagca                 <  1:738954/150‑1 (MQ=255)

                                                                                                                                       gAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTc                                                                  <  2:340006/100‑1 (MQ=255)

                                                                                                                                       gAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTc                                                                  >  1:340006/1‑100 (MQ=255)

                                                                                                                                       gAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGcagcag                >  2:1285859/1‑150 (MQ=255)

                                                                                                                                        aaCTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCagcagc               >  2:675817/1‑150 (MQ=255)

                                                                                                                                          cTGGCGCTGGAGCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTAc                       >  2:372387/1‑140 (MQ=255)

                                                                                                                                           tGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCg            >  1:318193/1‑150 (MQ=255)

                                                                                                                                            ggCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGt           <  1:1457378/150‑1 (MQ=255)

                                                                                                                                             gcgcTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTg          <  1:372318/150‑1 (MQ=255)

                                                                                                                                              cgcTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGc         <  1:1138757/150‑1 (MQ=255)

                                                                                                                                               gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                          <  1:1552989/84‑1 (MQ=255)

                                                                                                                                               gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGt                                                                          >  2:1552989/1‑84 (MQ=255)

                                                                                                                                               gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTc             >  1:1480034/1‑145 (MQ=255)

                                                                                                                                               gctgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTc             <  2:1480034/145‑1 (MQ=255)

                                                                                                                                                 tgctCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTgg      >  1:180251/1‑150 (MQ=255)

                                                                                                                                                     ccAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTGGTCCt  >  1:1058530/1‑150 (MQ=255)

                                                                                                                                                     ccAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTGGTCCt  >  1:1919813/1‑150 (MQ=255)

CCCGGTCTTTTCCGCCAGGCCGATGAAGCGGTCAGGCAGCAGCAGGCCCTGCTGCGGGTGCTCCCAGCGCAGCAACGCTTCGGCACCGATCGGTTGGCAGGCCTGGGCGTCGAACTTGGGCTGGTAATGCAAGCGGAACTGGCGCTGCTCCAGCGCCAGGCGCAAGTCCTGCAGCAGCTGCAACTGCTGGCGCGCATTGCTGTTCATCGACACATCGAAGAAGCTGTAACCGTTCTTGCCGGCGCTTTTGGCATGGTACATGGCAGCGTCGGCGTTACGCAGCAGCTCGTGCTGGTCCT  >  NC_002947/4062898‑4063196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`