Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 367,023 G→T 6.8% R99S (CGC→AGC)  PP_5442 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947367,0230GT 6.8% 229.1 / 3.7 74R99S (CGC→AGC) PP_5442hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (34/35);  new base T (3/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

ATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGAGGCTGGGT  >  NC_002947/366875‑367170
                                                                                                                                                    |                                                                                                                                                   
aTCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGa                                                                                                                                                    <  2:1512211/150‑1 (MQ=255)
       ccGGGACCTGGTCCCCCTCATGGTCCATTTCGGACAAGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCTTTTTTCGCGAGGCcggg                                                                                                                                             >  1:1716420/1‑150 (MQ=255)
       ccGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTCCGTGCCGACTGCAGAGCGGCTTGCACCCTTTAGTTCGGGCGCGTGAACCAAGGCCCTTTTTCGCGAGGCgggg                                                                                                                                             >  1:521051/1‑150 (MQ=255)
       ccGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCgggg                                                                                                                                             <  1:310897/150‑1 (MQ=255)
          ggACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTa                                                                                                                                          <  1:1464144/150‑1 (MQ=255)
             ccTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGaa                                                                                                                                       >  1:742140/1‑150 (MQ=255)
              cTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGaaa                                                                                                                                      >  1:1903657/1‑150 (MQ=255)
               tGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAATAGGCGCTCATGACGGTGTAGGGACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAg                                                                                                                                     <  2:1716420/150‑1 (MQ=255)
                  tCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCa                                                                                                                                  <  1:1752719/150‑1 (MQ=255)
                    ccccTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACt                                                                                                                                <  1:326080/150‑1 (MQ=255)
                      ccTCATGGTCCATTTCGATACTTAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGt                                                                                                                              >  2:318529/1‑150 (MQ=255)
                      ccTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGt                                                                                                                              >  1:967525/1‑150 (MQ=255)
                       cTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTa                                                                                                                             <  1:761478/150‑1 (MQ=255)
                        tCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTac                                                                                                                            >  2:14114/1‑150 (MQ=255)
                             gTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGa                                                                                                                                                    <  2:591457/121‑1 (MQ=255)
                             gTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGa                                                                                                                                                    >  1:591457/1‑121 (MQ=255)
                                cATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCt                                                                                                                    <  2:1902545/150‑1 (MQ=255)
                                    tCGATACTTAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAAt                                                                                                                <  1:318529/150‑1 (MQ=255)
                                    tCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAAt                                                                                                                <  2:2142836/150‑1 (MQ=255)
                                        tACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATgcaa                                                                                                            <  1:112904/150‑1 (MQ=255)
                                        tACTGAGATGTGAAGAGGCGTTCATGACGCTGTAGGTTCGTGTTCCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGTAAGCCACTGTACACATCGCTTAATgcaa                                                                                                            <  2:775091/150‑1 (MQ=255)
                                           tGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATgcaagca                                                                                                         >  2:1921173/1‑150 (MQ=255)
                                            gagaTGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATgcaagcaa                                                                                                        >  1:2166576/1‑150 (MQ=255)
                                            gagaTGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATgcaagcaa                                                                                                        >  1:954244/1‑150 (MQ=255)
                                               aTGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTg                                                                                                     >  2:1901489/1‑150 (MQ=255)
                                                 gtgAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCgg                                                                                                                                               >  2:1587914/1‑106 (MQ=255)
                                                 gtgAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCgg                                                                                                                                               <  1:1587914/106‑1 (MQ=255)
                                                  tgAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCgg                                                                                                  <  2:1321516/150‑1 (MQ=255)
                                                   gAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAgg                                                                                                                                                  <  2:1134460/101‑1 (MQ=255)
                                                   gAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAgg                                                                                                                                                  >  1:1134460/1‑101 (MQ=255)
                                                      gagGCGTGCAGGACGGTGAAGGTACGCTTACCGGCCGACTGCAGAGCGGCTTGAACATGTTCGTAAAAAGTTTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCa                                                                                              <  1:1210504/150‑1 (MQ=255)
                                                           gTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAGTGCAAGCAAGTGCGGCTCATacac                                                                                         >  2:792327/1‑150 (MQ=255)
                                                             tCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACGCaa                                                                                       <  2:1903657/150‑1 (MQ=255)
                                                                    ggTGAAGGTACGCGTACCTGCCGACTGCCGAGCGGCTTGAACATTTTCGTCAAGGCTCTTGAACCAAGGACCCAGTTCGCGAGGTGGGGGAAGAAAGCCACAGTACACATAGTTTAATGCAAGAAAGTGCGGATCATACAAAATGGtttt                                                                                >  1:1106966/1‑150 (MQ=255)
                                                                    ggTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGc                                                                                                                                    >  1:616442/1‑98 (MQ=255)
                                                                    ggTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGc                                                                                                                                    <  2:616442/98‑1 (MQ=255)
                                                                        aaGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAGTGCAAGCAAGTGCGGCTCATACACAATGGTTTTAtgt                                                                            <  1:792327/150‑1 (MQ=255)
                                                                           gTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTa                                                                         >  1:1089813/1‑150 (MQ=255)
                                                                               gcgTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGtt                                                                     >  2:1743359/1‑150 (MQ=255)
                                                                                cgTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTc                                                                    <  2:2181464/150‑1 (MQ=255)
                                                                                cgTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTc                                                                    <  2:521051/150‑1 (MQ=255)
                                                                                 gTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCg                                                                   >  2:2255398/1‑150 (MQ=255)
                                                                                       gCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAgg                                                             <  1:14114/150‑1 (MQ=255)
                                                                                             tGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAAt                                                       >  1:62975/1‑150 (MQ=255)
                                                                                                  agCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCaa                                                  <  2:62975/150‑1 (MQ=255)
                                                                                                   gCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAAt                                                 <  1:2255398/150‑1 (MQ=255)
                                                                                                      gCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATGCACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGAt                                              >  2:1418046/1‑150 (MQ=255)
                                                                                                      gCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGAt                                              >  2:172629/1‑150 (MQ=255)
                                                                                                       cTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTTTTTTTTTTTTTATAGAAAAACACTGTACACATCACTTAAAGCAAGCAAGTGCGGCTCATACTCAATTGTTTTTTGTTTATGTTCGATTAGGGTGAATTTCAATGAtt                                             >  2:273819/1‑150 (MQ=255)
                                                                                                           aaCATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTc                                                                    <  1:1459142/123‑1 (MQ=255)
                                                                                                           aaCATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTc                                                                    >  2:1459142/1‑123 (MQ=255)
                                                                                                            aCATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAg                                        >  2:730574/1‑150 (MQ=255)
                                                                                                              aTTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCTAGGCGGGGGTAGAAAGCCACTGTACACATCGCt                                                                                                                    >  2:1027788/1‑72 (MQ=255)
                                                                                                              aTTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCTAGGCGGGGGTAGAAAGCCACTGTACACATCGCt                                                                                                                    <  1:1027804/72‑1 (MQ=255)
                                                                                                              aTTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCTAGGCGGGGGTAGAAAGCCACTGTACACATCGCt                                                                                                                    <  1:1027788/72‑1 (MQ=255)
                                                                                                              aTTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCTAGGCGGGGGTAGAAAGCCACTGTACACATCGCt                                                                                                                    >  2:1027804/1‑72 (MQ=255)
                                                                                                              aTTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGt                                      >  2:1185104/1‑150 (MQ=255)
                                                                                                                     tcgAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATa                               >  1:119087/1‑150 (MQ=255)
                                                                                                                       gAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATac                                                                                           <  2:1558214/88‑1 (MQ=255)
                                                                                                                       gAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATac                                                                                           >  1:1558214/1‑88 (MQ=255)
                                                                                                                       gAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCCGGCAGGGTGCGTTTTAAAGAGAGCAAGGTGCCGAGAGc                             >  2:1666824/1‑150 (MQ=255)
                                                                                                                                gAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATATTTAATGGTTTTATGTGTATGTTCGAGGAGGGTGAATTTCAATGATTGCTAGGTGGCGATAGCGACAGTGac                    <  2:1106966/150‑2 (MQ=255)
                                                                                                                                gAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGaa                    <  1:2133632/150‑1 (MQ=255)
                                                                                                                                 aaCCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAAc                   <  1:1743359/150‑1 (MQ=255)
                                                                                                                                    cAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCAt                >  2:1623140/1‑150 (MQ=255)
                                                                                                                                       ggCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGTGACAGTGAACCATTGc             <  1:1217764/150‑1 (MQ=255)
                                                                                                                                         cccTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGTCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTg           >  2:258767/1‑150 (MQ=255)
                                                                                                                                          ccTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGAtt                                             >  1:1053362/1‑115 (MQ=255)
                                                                                                                                          ccTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGAtt                                             <  2:1053362/115‑1 (MQ=255)
                                                                                                                                          ccTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGa          >  2:2194270/1‑150 (MQ=255)
                                                                                                                                          ccTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATAACTGGATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGa          >  1:1448985/1‑150 (MQ=255)
                                                                                                                                            tttttCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGAgg        <  1:1666824/150‑1 (MQ=255)
                                                                                                                                                tCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGAGGCTgg    <  1:2194270/150‑1 (MQ=255)
                                                                                                                                                tCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGAGGCTgg    <  2:2166576/150‑1 (MQ=255)
                                                                                                                                                  gcgAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATgtgt                                                                          <  1:756697/78‑1 (MQ=255)
                                                                                                                                                  gcgAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATgtgt                                                                          >  2:756697/1‑78 (MQ=255)
                                                                                                                                                  gcgAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGt                       <  2:2218260/129‑1 (MQ=255)
                                                                                                                                                  gcgAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGt                       >  1:2218260/1‑129 (MQ=255)
                                                                                                                                                  gcgAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGAGGCTGGGt  >  2:1361124/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCGCAACCGGGACCTGGTCCCCCTCATGGTCCATTTCGATACTGAGATGTGAAGAGGCGTTCATGACGGTGAAGGTACGCGTACCTGCCGACTGCAGAGCGGCTTGAACATTTTCGTCGAGGCTCTTGAACCAAGGCCCTTTTTCGCGAGGCGGGGGTAGAAAGCCACTGTACACATCGCTTAATGCAAGCAAGTGCGGCTCATACACAATGGTTTTATGTGTATGTTCGAGCAGGGTGAATTTCAATGATTGCAAGGTGGCGATAGCGACAGTGAACCATTGCTGAGGCTGGGT  >  NC_002947/366875‑367170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑