Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 418,288 T→C 6.9% V23A (GTG→GCG)  PP_0345 → heptose kinase WapP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947418,2880TC 6.9% 240.9 / 4.1 82V23A (GTG→GCG) PP_0345heptose kinase WapP
Reads supporting (aligned to +/- strand):  ref base T (40/36);  new base C (2/4);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCGCGCTGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGG  >  NC_002947/418143‑418434
                                                                                                                                                 |                                                                                                                                                  
aGCCGCGCTGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTc                                                                                                                                                <  2:899892/150‑1 (MQ=255)
    gcgcTGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCCAGGAATGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGccc                                                                                                                                            <  1:1093179/150‑1 (MQ=255)
    gcgcTGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGccc                                                                                                                                            >  1:251408/1‑150 (MQ=255)
      gctgctCGCCTGGCTGGAGCCCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTc                                                                                                                                          <  1:501784/150‑1 (MQ=255)
        tgctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCa                                                                                                                                        >  2:719585/1‑150 (MQ=255)
          ctCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGCACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                      <  2:161121/150‑1 (MQ=255)
                tggctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAc                                                                                                                                <  2:867775/150‑1 (MQ=255)
                  gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                              <  1:1157944/150‑1 (MQ=255)
                  gctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCCGCAGCCTCGACGCGGCGTTCGCCCTGGAGGGCGAAcac                                                                                                                              <  1:1805227/150‑3 (MQ=255)
                    tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCt                                                                                                                            >  2:1106007/1‑150 (MQ=255)
                            aaGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcg                                                                                                                    >  1:586936/1‑150 (MQ=255)
                              ggCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGGACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTTTTCGCCCTCCAGGGCGAACGGCTgacccgcgac                                                                                                                  <  1:1938427/150‑1 (MQ=255)
                               gCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCt                                                                                                                            <  2:2167620/139‑1 (MQ=255)
                               gCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCt                                                                                                                            >  1:2167620/1‑139 (MQ=255)
                                  cAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccgc                                                                                                              >  1:1790031/1‑150 (MQ=255)
                                       acgctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTTGCGGCGGACTTCGGCAGCCTCGACGCGGCGTTCGCCCTGGAGGGCGAAcac                                                                                                                              <  2:1182102/126‑3 (MQ=255)
                                       aCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTTGCGGCGGACTTCGGCAGCCTCGACGCGGCGTTCGCCATGGAGGGCGAAcac                                                                                                                              >  1:1182102/1‑127 (MQ=255)
                                         tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGaa                                                                                                                                 <  1:286048/124‑1 (MQ=255)
                                         tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGaa                                                                                                                                 >  2:286048/1‑124 (MQ=255)
                                           tACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGCGTTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAg                                                                                                     >  1:850661/1‑150 (MQ=255)
                                             cGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAggg                                                                                                                                     <  2:1349426/116‑1 (MQ=255)
                                             cGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAggg                                                                                                                                     >  1:1349426/1‑116 (MQ=255)
                                                tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACCCTTGCGCCGGTGTACGAACACCTGGCGAAGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTa                                                                                                <  1:1685881/150‑1 (MQ=255)
                                                tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAGGGCTGACTCGCGACCCGCTCAGTGAAGTGGTa                                                                                                >  2:723282/1‑150 (MQ=255)
                                                tCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTa                                                                                                <  1:2121793/150‑1 (MQ=255)
                                                  gcaagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACGTCGGCAGCATCGACGCGGTGTTCGCCCTGCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACg                                                                                              >  1:1323572/1‑150 (MQ=255)
                                                    aagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCa                                                                                            <  2:251408/150‑1 (MQ=255)
                                                      gcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATc                                                                                          <  1:2092653/150‑1 (MQ=255)
                                                          cGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGc                                                                                      <  1:1106007/150‑1 (MQ=255)
                                                          cGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGCGTTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGc                                                                                      <  2:850661/150‑1 (MQ=255)
                                                              aTGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGa                                                                                  <  2:756431/150‑1 (MQ=255)
                                                                       cACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCaa                                                                         >  2:1809372/1‑150 (MQ=255)
                                                                        aCTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                        >  2:2165238/1‑150 (MQ=255)
                                                                         ctctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAact                                                                       <  1:506473/150‑1 (MQ=255)
                                                                         ctctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAact                                                                       >  1:555779/1‑150 (MQ=255)
                                                                          tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAacta                                                                      >  2:476279/1‑150 (MQ=255)
                                                                          tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAacta                                                                      <  1:701823/150‑1 (MQ=255)
                                                                              aTGGCGAGGTGGACACTGGCGCCGGGGTACGAACACCTGGGGGCGGACTGCGGCAGCCTCGAGGCGGTGTTCGCCCTCCAGGGCGAACGGCTAACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACtactac                                                                  >  2:690752/1‑150 (MQ=255)
                                                                                ggCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGt                                                                >  2:203877/1‑150 (MQ=255)
                                                                                 gagAGTTGGATCCTGGCGCCGGGATACGACCTCCTGGCTGCGGAATTCGGCAGCCACGACGCGGTGTCCGCCCTACAGGGCGTACCGCTGACCCGCGACCCGCTCAGTGAAGTGGTACACATCGAGCTTGACGGGATCAACTACTACGTc                                                               <  2:152768/148‑1 (MQ=255)
                                                                                   gAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAacta                                                                      >  2:1988596/1‑141 (MQ=255)
                                                                                   gAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAacta                                                                      <  1:1988596/141‑1 (MQ=255)
                                                                                     gTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAg                                                                                                                                       <  1:1800961/74‑1 (MQ=255)
                                                                                     gTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAg                                                                                                                                       >  2:1800961/1‑74 (MQ=255)
                                                                                         gACACTGGCGCCGGGGTACGAAGACCTGGCGGCGGACTTCGGCAGCCTCGACGGGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGAAAGGATCAAGTACTACGTCAAgagata                                                       >  2:846411/1‑145 (MQ=255)
                                                                                          acacTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTac                                                      >  1:925365/1‑150 (MQ=255)
                                                                                           cacTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGAt                                                                            >  1:1906906/1‑127 (MQ=255)
                                                                                           cacTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGAt                                                                            <  2:1906906/127‑1 (MQ=255)
                                                                                             cTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACAcc                                                   <  2:1790031/150‑1 (MQ=255)
                                                                                              tGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCg                                                  >  2:424357/1‑150 (MQ=255)
                                                                                                 cgcCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCgggg                                               <  1:203877/150‑1 (MQ=255)
                                                                                                        gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCt                                                                                                             >  2:1986600/1‑81 (MQ=255)
                                                                                                        gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCt                                                                                                             <  1:1986600/81‑1 (MQ=255)
                                                                                                         tACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAg                                       >  1:2073470/1‑150 (MQ=255)
                                                                                                            gAACCCCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGTGGCCGGCAAGcac                                    <  1:729612/150‑1 (MQ=255)
                                                                                                                acCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGTGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgc                                >  1:1094965/1‑150 (MQ=255)
                                                                                                                acCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGc                                          <  2:896420/140‑1 (MQ=255)
                                                                                                                acCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGc                                          >  1:896420/1‑140 (MQ=255)
                                                                                                                  cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgcgc                              >  2:2147027/1‑150 (MQ=255)
                                                                                                                  cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGCACGCATCGAGCGTGACGGGATCAACTACTACGTCaa                                                             >  1:1668724/1‑119 (MQ=255)
                                                                                                                  cTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGCACGCATCGAGCGTGACGGGATCAACTACTACGTCaa                                                             <  2:1668724/119‑1 (MQ=255)
                                                                                                                   tGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGc                                                                                             <  2:942368/86‑1 (MQ=255)
                                                                                                                   tGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGc                                                                                             >  1:942368/1‑86 (MQ=255)
                                                                                                                   tGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGcc                             >  2:2047731/1‑150 (MQ=255)
                                                                                                                    ggcggcGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAAGGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCg                            <  1:723282/150‑1 (MQ=255)
                                                                                                                    ggcggcGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGGGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCg                            <  1:719585/150‑1 (MQ=255)
                                                                                                                       ggcggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg                                                >  2:1453286/1‑127 (MQ=255)
                                                                                                                       ggcggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCggg                                                <  1:1453286/127‑1 (MQ=255)
                                                                                                                        gcggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTAc                        <  1:2147027/150‑1 (MQ=255)
                                                                                                                         cggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTAcc                       >  1:1843554/1‑150 (MQ=255)
                                                                                                                          ggACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCt                      >  2:1920777/1‑150 (MQ=255)
                                                                                                                           gACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTa                                                       >  1:1832501/1‑116 (MQ=255)
                                                                                                                           gACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTa                                                       <  2:1832501/116‑1 (MQ=255)
                                                                                                                           gACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTg                     >  1:1594972/1‑150 (MQ=255)
                                                                                                                             cTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTggg                   >  1:628751/1‑150 (MQ=255)
                                                                                                                                 ggtaGCCGCGGCGCGACGTTCCGCCGCCAGGGCTGACTCCTGAGCTGTGACCTGCGTAGTGAAGTTGTACGCATGGTGTGTGACGTGATCAACTTTTACGTCAAGCGCTACACCGGTGCCGGCGAGCACATGCGCCGGTACCTGTggcgg               <  2:172665/147‑1 (MQ=255)
                                                                                                                                       cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATAGAGGGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGc         >  2:2199457/1‑150 (MQ=255)
                                                                                                                                        tCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACTCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCa        >  1:162452/1‑150 (MQ=255)
                                                                                                                                        tCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCa        >  2:1941083/1‑150 (MQ=255)
                                                                                                                                              cgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCTCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg  >  2:1854422/1‑150 (MQ=255)
                                                                                                                                              cgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg  <  2:586936/150‑1 (MQ=255)
                                                                                                                                              cgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg  <  1:1809372/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGCCGCGCTGCTCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGG  >  NC_002947/418143‑418434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑