Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,475,342	C→A	5.4%	D80Y (GAC→TAC)	PP_3964 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,475,342	0	C	A	5.4%	232.3 / 2.7	74	D80Y (GAC→TAC)	PP_3964	transposase
Reads supporting (aligned to +/- strand):  ref base C (34/36);  new base A (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

ACAGACGCACTGGGTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCTCATCGC  >  NC_002947/4475193‑4475489                                                                                                                                                      |                                                                                                                                                    acaGACGCACTGGGTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTc                                                                                                                                                     >  2:1076663/1‑150 (MQ=255)  caGACGCACTGGGTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCt                                                                                                                                                    <  1:223096/150‑1 (MQ=255)    gACGCACTGGGTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCtgt                                                                                                                                                  >  2:1010182/1‑150 (MQ=255)       gCACTGGGTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTc                                                                                                                                               <  2:674889/150‑1 (MQ=255)         aCTGGGTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGa                                                                                                                                             >  2:686061/1‑150 (MQ=255)             ggTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAg                                                                                                                                         <  1:686061/150‑1 (MQ=255)                gtTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTc                                                                                                                                      <  2:1798937/150‑1 (MQ=255)                 ttttCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTTGATAGCTTCAAGGTCTGTGTCGAGCAGGTCa                                                                                                                                     <  1:2057205/150‑1 (MQ=255)                    tCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATcc                                                                                                                                  >  1:777744/1‑150 (MQ=255)                     cGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCa                                                                                                                                 <  1:1689979/150‑1 (MQ=255)                                 gAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCttt                                                                                                                     >  2:801614/1‑150 (MQ=255)                                    tCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTCGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTgcg                                                                                                                  >  2:550715/1‑150 (MQ=255)                                     cACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTgcgc                                                                                                                 <  2:1656467/150‑1 (MQ=255)                                               ggAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCttt                                                                                                                     >  2:960781/1‑136 (MQ=255)                                               ggAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCttt                                                                                                                     <  1:960781/136‑1 (MQ=255)                                                gAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAATCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGAttt                                                                                                      >  2:1948302/1‑150 (MQ=255)                                                gAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCTAGCAAGTTGCTTTGCGCCGGGCTGAttt                                                                                                      <  1:1010182/150‑1 (MQ=255)                                                  aaCTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGc                                                                                                    <  2:308053/150‑1 (MQ=255)                                                    cTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTc                                                                                                  >  2:1427645/1‑150 (MQ=255)                                                           ggcAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATACCGCACGTTGCCTTGCGCCGGGCTGATTTGCTCGCTgcgc                                                                                           <  1:1221069/150‑1 (MQ=255)                                                            gcAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCt                                                                                          >  1:2091596/1‑150 (MQ=255)                                                             cAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCTGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCtt                                                                                         <  1:1678924/150‑1 (MQ=255)                                                              aaaGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTg                                                                                        >  2:1848502/1‑150 (MQ=255)                                                              aaaGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTg                                                                                        >  1:239581/1‑150 (MQ=255)                                                              aaaGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTg                                                                                        <  1:1611287/150‑1 (MQ=255)                                                                      aCGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACtt                                                                                <  2:1106264/150‑1 (MQ=255)                                                                        gTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTtgt                                                                              <  1:572359/150‑1 (MQ=255)                                                                         tttCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAATCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTtgtg                                                                             <  1:1948302/150‑1 (MQ=255)                                                                         tttCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTtgtg                                                                             >  1:473032/1‑150 (MQ=255)                                                                          ttCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGc                                                                            <  1:1427645/150‑1 (MQ=255)                                                                              gggATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTg                                                                                        >  2:1204989/1‑134 (MQ=255)                                                                              gggATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTg                                                                                        <  1:1204989/134‑1 (MQ=255)                                                                                 aTTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGa                                                                     <  2:239581/150‑1 (MQ=255)                                                                                 aTTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGATTGGCAAACTTGTGCCGTTTGa                                                                     <  1:2059739/150‑1 (MQ=255)                                                                                      cGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAgg                                                                <  1:1076663/150‑1 (MQ=255)                                                                                      cGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAgg                                                                <  1:1848502/150‑1 (MQ=255)                                                                                       gTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAggg                                                               >  1:881829/1‑150 (MQ=255)                                                                                        tGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGAACGGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGc                                                              <  1:840726/150‑1 (MQ=255)                                                                                               gCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATc                                                                                                                                   >  1:281532/1‑74 (MQ=255)                                                                                               gCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATc                                                                                                                                   <  2:281532/74‑1 (MQ=255)                                                                                                 cTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTGGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGaa                                                     <  2:141722/150‑1 (MQ=255)                                                                                                 cTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGaa                                                     >  2:1826557/1‑150 (MQ=255)                                                                                                    tgGCGCATCTGGGAGAAGCTGTTTTGGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACtt                                                  >  1:465995/1‑150 (MQ=255)                                                                                                             tGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAgg                                                                                                                                        >  2:674262/1‑55 (MQ=255)                                                                                                             tGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAgg                                                                                                                                        <  1:674262/55‑1 (MQ=255)                                                                                                             tGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTc                                         >  2:1342422/1‑150 (MQ=255)                                                                                                             tGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGCCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTc                                         >  1:2231340/1‑150 (MQ=255)                                                                                                              gggAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTATGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGt                                                                         <  1:1868045/117‑1 (MQ=255)                                                                                                              gggAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTATGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGt                                                                         <  1:1868283/117‑1 (MQ=255)                                                                                                              gggAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTATGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGt                                                                         >  2:1868045/1‑117 (MQ=255)                                                                                                              gggAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTATGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGt                                                                         >  2:1868283/1‑117 (MQ=255)                                                                                                                gagaAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGAt                                      >  2:316421/1‑150 (MQ=255)                                                                                                                     gCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTg                                                 <  1:1438373/134‑1 (MQ=255)                                                                                                                     gCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTg                                                 >  2:1438373/1‑134 (MQ=255)                                                                                                                              gCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGt                        <  1:316421/150‑1 (MQ=255)                                                                                                                               cTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGAAGCTCAAAAACATCGTCATCTGGCGGCGGGCTGAGTTGGTGGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGtt                       >  1:989721/1‑150 (MQ=14)                                                                                                                                 cGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCt                     <  2:979532/150‑1 (MQ=255)                                                                                                                                 cGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCt                     <  2:473032/150‑1 (MQ=255)                                                                                                                                 cGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTCCATCGTTCt                     <  2:2231340/150‑1 (MQ=255)                                                                                                                                     cTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAAt                 <  2:881829/150‑1 (MQ=255)                                                                                                                                        gATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCtt              >  2:237211/1‑150 (MQ=255)                                                                                                                                         aTAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTc             <  2:465995/150‑1 (MQ=255)                                                                                                                                           aGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTTGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCt           >  1:162484/1‑150 (MQ=255)                                                                                                                                             cTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGc         >  2:2038452/1‑150 (MQ=255)                                                                                                                                              ttCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTc             >  2:1841158/1‑145 (MQ=255)                                                                                                                                              ttCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTc             <  1:1841158/145‑1 (MQ=255)                                                                                                                                              ttCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCt        >  2:1445792/1‑150 (MQ=255)                                                                                                                                              ttCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCt        >  1:1672888/1‑150 (MQ=255)                                                                                                                                              ttCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAAAGGGTGCCAGTTGAGCAGGTCGATTGCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCt        >  2:1432610/1‑150 (MQ=17)                                                                                                                                                cAAGGTCTGTTTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCTCa      >  2:1240178/1‑150 (MQ=255)                                                                                                                                                 aaGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTc             >  2:194084/1‑142 (MQ=255)                                                                                                                                                 aaGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGCGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTc             <  1:194084/142‑1 (MQ=255)                                                                                                                                                   ggTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATc                <  2:1118713/137‑1 (MQ=255)                                                                                                                                                   ggTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATc                >  1:1118713/1‑137 (MQ=255)                                                                                                                                                   ggTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTT‑GATGAGGATTTCATCGTTCTGAATCTTCCTGCTCATCGc  >  1:2167588/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                    ACAGACGCACTGGGTGTTTTCGGGTTCGTGACGAATCACCGTGCGTGGAAACTGCGGCGGCAAAGGCGCACGTTTCGGGGATTGTCGTGGTTCGGCCTGTGGCGCATCTGGGAGAAGCTGTTTTAGCTCGGCCTCGATAGCTTCAAGGTCTGTGTCGAGCAGGTCATCCAGCAAGTTGCTTTGCGCCGGGCTGATTTGCTCGCTGCGCTTGGCAAACTTGTGCCGTTTGAGCAGGGCGATTTCGAACTTGAACTGTTCGATGAGGATTTCATCGTTCTGAATCTTCCTGCTCATCGC  >  NC_002947/4475193‑4475489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑