Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,724,029 A→G 5.2% F45L (TTC→CTC)  yigI ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,724,0290AG 5.2% 233.1 / 2.1 76F45L (TTC→CTC) yigIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (37/35);  new base G (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

AAAGTCGATGATCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATCATGCCTTGCCCTCCTTC  >  NC_002947/4723880‑4724176
                                                                                                                                                     |                                                                                                                                                   
aaaGTCGATGATCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCATTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGaa                                                                                                                                                     <  1:680231/150‑1 (MQ=255)
aaaGTCGATGATCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGaa                                                                                                                                                     <  2:1459117/150‑1 (MQ=255)
aaaGTCGATGATCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGaa                                                                                                                                                     <  2:1694514/150‑1 (MQ=255)
     cGATGATCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAacaaca                                                                                                                                                <  1:984419/150‑1 (MQ=255)
     cGATGATCTTCGGAATGCTTGCGCTCTCGCAGTACATCAGTAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTCGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAacaaca                                                                                                                                                <  2:1353189/150‑1 (MQ=255)
      gatgatCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAacac                                                                                                                                               <  1:267900/150‑1 (MQ=255)
      gatgatCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAacac                                                                                                                                               >  2:1964954/1‑150 (MQ=255)
        tgatCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGaacaac                                                                                                                                                 >  1:1310325/1‑146 (MQ=255)
        tgatCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGaacaac                                                                                                                                                 <  2:1310325/146‑1 (MQ=255)
            cTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATc                                                                                                                                         <  2:1271733/150‑1 (MQ=255)
                ggAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGccc                                                                                                                                     <  2:1000231/150‑1 (MQ=255)
                      cTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGc                                                                                                                               >  1:2238327/1‑150 (MQ=255)
                       ttGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCt                                                                                                                              >  2:295433/1‑150 (MQ=255)
                                 gCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGAt                                                                                                                    <  2:1495803/150‑1 (MQ=255)
                                  cTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATg                                                                                                                   >  2:1708502/1‑150 (MQ=255)
                                  cTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATg                                                                                                                   <  1:1708502/150‑1 (MQ=255)
                                   tGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGc                                                                                                                  <  2:1025386/150‑1 (MQ=255)
                                     tAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATc                                                                                                                                         >  2:226238/1‑125 (MQ=255)
                                     tAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATc                                                                                                                                         <  1:226238/125‑1 (MQ=255)
                                       gATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGaa                                                                                                                                                     <  2:1130558/111‑1 (MQ=255)
                                       gATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGaa                                                                                                                                                     >  1:1130558/1‑111 (MQ=255)
                                            gcagGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGcc                                                                                                         >  2:692588/1‑150 (MQ=255)
                                              agGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCg                                                                                                       >  1:359352/1‑150 (MQ=255)
                                                gTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGc                                                                                                     >  2:2041082/1‑150 (MQ=255)
                                                   cAGCGCTGCCGACAGCTCCATGAAGAGCTGGAGCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATa                                                                                                  <  1:1755661/150‑1 (MQ=255)
                                                    aGCGTTGCCGACAGCTCCATGAAGCCGGCGATCACGCCCGCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAg                                                                                                 <  1:122579/150‑1 (MQ=255)
                                                     gcgcTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCAGATAGTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAgg                                                                                                <  1:404028/150‑1 (MQ=255)
                                                      cgcTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATc                                                                                                             >  1:762404/1‑136 (MQ=255)
                                                      cgcTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATc                                                                                                             <  2:762404/136‑1 (MQ=255)
                                                      cgcTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAggg                                                                                               <  2:259922/150‑1 (MQ=255)
                                                         tGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAAt                                                                                            <  1:402424/150‑1 (MQ=255)
                                                           ccGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCa                                                                                          <  2:2238327/150‑1 (MQ=255)
                                                             gACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAgc                                                                                        <  1:1900335/150‑1 (MQ=255)
                                                             gACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAgc                                                                                        <  1:295433/150‑1 (MQ=255)
                                                               cAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAgcgc                                                                                      >  2:429569/1‑150 (MQ=255)
                                                                  ccccATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCGGTTTTTGTCCGGGCGGGCCGGCAGGCGAAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATGGGGAATCAGCCCCAg                                                                                   <  2:1410282/148‑1 (MQ=255)
                                                                     cATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCaa                                                                                >  1:265057/1‑150 (MQ=255)
                                                                     cATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTCGCCGGCGGGCGGAAAAACACGTCATGGCCCGGACGCTCGCATTGGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCaa                                                                                >  2:1431011/1‑150 (MQ=255)
                                                                         aaGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGt                                                                            <  2:1415019/150‑1 (MQ=255)
                                                                             cGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGAGCAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGAt                                                                        >  2:2304052/1‑150 (MQ=255)
                                                                              ggCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATa                                                                       >  1:1663254/1‑150 (MQ=255)
                                                                               gCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAg                                                                      <  2:265057/150‑1 (MQ=255)
                                                                                 gATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTc                                                                    >  1:542450/1‑150 (MQ=255)
                                                                                 gATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTc                                                                    <  1:2041082/150‑1 (MQ=255)
                                                                                    cACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGcc                                                                 <  1:1964954/150‑1 (MQ=255)
                                                                                     aCGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGccc                                                                >  1:791067/1‑150 (MQ=255)
                                                                                         cgccATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGccc                                                                                                                                     <  1:1412664/77‑1 (MQ=255)
                                                                                         cgccATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGccc                                                                                                                                     >  2:1412664/1‑77 (MQ=255)
                                                                                         cgccATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGTCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCatgc                                                            >  2:1599911/1‑147 (MQ=255)
                                                                                          gccATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGc                                                           <  1:692588/150‑1 (MQ=255)
                                                                                             atggatggCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATg                                                        <  2:359352/150‑1 (MQ=255)
                                                                                             atggatggCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGAAGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTGGATAGTCGCCCACCGCATg                                                        >  1:987722/1‑150 (MQ=255)
                                                                                               ggatggCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATggg                                                      <  2:1678132/150‑1 (MQ=255)
                                                                                                      cGGTAGCAGTGGGTTGCCGATATTTTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGtt                                               >  1:714682/1‑150 (MQ=255)
                                                                                                       ggTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGAGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTg                                              <  1:1199923/150‑1 (MQ=255)
                                                                                                        gTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAGCAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGa                                             >  2:969891/1‑150 (MQ=255)
                                                                                                        gTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAGCAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGa                                             >  2:970206/1‑150 (MQ=255)
                                                                                                          aGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAgc                                           <  2:115925/150‑1 (MQ=255)
                                                                                                             aGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCACCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAgctgc                                        <  2:1012256/150‑1 (MQ=255)
                                                                                                              gTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCa                                                               <  1:279344/126‑1 (MQ=255)
                                                                                                              gTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCa                                                               >  2:279344/1‑126 (MQ=255)
                                                                                                                gggTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTgg                                     >  2:2325193/1‑150 (MQ=255)
                                                                                                                gggTTGCCCATATTGTCCGGGCTGGCCGGCAGGCGGAACAACCCGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAGTCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTgg                                     <  2:569019/150‑1 (MQ=255)
                                                                                                                  gTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCg                                   >  1:1756369/1‑150 (MQ=255)
                                                                                                                      ccGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTAcc                               >  1:631746/1‑150 (MQ=255)
                                                                                                                      ccGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTAGATGGGCGGCCACCTCAGTGGCGGCGGTGCGCTGCTGCCATAcc                               >  2:382518/1‑150 (MQ=255)
                                                                                                                       cGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTc                                                                    >  1:1853008/1‑112 (MQ=255)
                                                                                                                       cGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTc                                                                    <  2:1853008/112‑1 (MQ=255)
                                                                                                                        gATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATTAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGtt                                               >  1:1981384/1‑132 (MQ=255)
                                                                                                                        gATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATTAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGtt                                               <  2:1981384/132‑1 (MQ=255)
                                                                                                                        gATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACcgc                             >  1:1026303/1‑150 (MQ=255)
                                                                                                                              gTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCgg                       >  1:312076/1‑150 (MQ=255)
                                                                                                                               tCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGa                      >  2:1235561/1‑150 (MQ=255)
                                                                                                                                 cGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGt                                                >  1:868511/1‑122 (MQ=255)
                                                                                                                                 cGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGt                                                <  2:868511/122‑1 (MQ=255)
                                                                                                                                  gggCTGGCCGGCAGGAGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGCCGTACCGCGTGCGGAATc                   <  1:2304052/150‑1 (MQ=255)
                                                                                                                                     cTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATCATg                >  1:86626/1‑150 (MQ=255)
                                                                                                                                      tGGCCGGCACGGGGGACAACACATCATCGCCCTGACGCTCGCATGCGATGCCGACCAGCCCGGCCTAGGGAATCAGCGCGAGCAACGGTAGATCGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATAATGc               >  2:2234381/1‑150 (MQ=255)
                                                                                                                                       ggCCGGCAGGCGGAGCAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATCATGcc              <  1:969891/150‑1 (MQ=255)
                                                                                                                                       ggCCGGCAGGCGGAGCAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATCATGcc              <  1:970206/150‑1 (MQ=255)
                                                                                                                                         ccGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATCATGCCtt            >  2:1020558/1‑150 (MQ=255)
                                                                                                                                          cGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTAACGCGTGCGGAATCATGCCTTg           >  1:1925717/1‑150 (MQ=255)
                                                                                                                                                  ggAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTCAGCTATCATGCCTTGCCCTCCtt   <  2:1377247/150‑1 (MQ=255)
                                                                                                                                                  ggAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCACCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTCGAGCTGCTGGCGTACCGCGTGCGGAATCATGCCTTGCCCTCCtt   >  2:1683039/1‑150 (MQ=255)
                                                                                                                                                   gAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATCATGCCTTGCCCTCCTTc  >  1:469444/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAAGTCGATGATCTTCGGAATGCTTGCGCTCTCGCTGTAGATCAGCAGGTACAGCGCTGCCGACAGCTCCATGAAGCCGGCGATCACGCCGCCATGGATGGCCGGTAGCAGTGGGTTGCCGATATTGTCCGGGCTGGCCGGCAGGCGGAACAACACGTCATCGCCCTGACGCTCGCATTCGATGCCGATCAGCCCGGCATAGGGAATCAGCGCCAGCAACGGTCGATAGTCGCCCACCGCATGGGCGGCGTTGAGCTGCTGGCGTACCGCGTGCGGAATCATGCCTTGCCCTCCTTC  >  NC_002947/4723880‑4724176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑