Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,906,511	A→T	8.2%	L111Q (CTG→CAG)	PP_4315 ←	epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,906,511	0	A	T	8.2%	214.2 / 10.3	73	L111Q (CTG→CAG)	PP_4315	epimerase
Reads supporting (aligned to +/- strand):  ref base A (32/35);  new base T (3/3);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGAA  >  NC_002947/4906365‑4906659                                                                                                                                                   |                                                                                                                                                     tttCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGc                                                                                                                                                   <  2:223326/150‑1 (MQ=255) tttCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGc                                                                                                                                                   <  2:389824/150‑1 (MQ=255)     gAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATc                                                                                                                                               >  2:184046/1‑150 (MQ=255)       gcagcaTGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCTGCTGGCCAtgga                                                                                                                                             <  1:979773/150‑4 (MQ=255)              ggCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGTGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAgg                                                                                                                                                    <  2:1721795/136‑1 (MQ=255)              ggCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGTGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAgg                                                                                                                                                    >  1:1721795/1‑136 (MQ=255)              ggCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACg                                                                                                                                      <  1:200985/150‑1 (MQ=255)               gCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACgg                                                                                                                                     >  2:1464306/1‑150 (MQ=255)               gCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACgg                                                                                                                                     <  1:357914/150‑1 (MQ=255)                cGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGc                                                                                                                                    <  1:1245457/150‑1 (MQ=255)                          caccacGACATAATCGTCGCTAAGGTACACCGCCTTTGTCTTGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCTATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCaa                                                                                                                          <  2:1354741/150‑1 (MQ=255)                            ccacGACATAATCGTCGCTAAGGTAGACCGCATTAGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACt                                                                                                                        >  1:1332961/1‑150 (MQ=255)                             cacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTc                                                                                                                       >  2:2025346/1‑150 (MQ=255)                             cacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTc                                                                                                                       >  1:1640473/1‑150 (MQ=255)                             cacGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCACTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCATGCCATCAGCGCCACGGCTGACGCGCAACTc                                                                                                                       <  2:486380/150‑1 (MQ=255)                                     aaTCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGc                                                                                                               >  2:367832/1‑150 (MQ=255)                                       tcgtcgCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCgg                                                                                                             <  1:367832/150‑1 (MQ=255)                                       tcgtcgCTAAGGTACAACGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCgg                                                                                                             >  1:580949/1‑150 (MQ=255)                                       tcgtccCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGAAAGATTCCGACCACGACCGCTACGGGTTGTTTGGCGTGGACGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCgg                                                                                                             <  1:1112485/150‑1 (MQ=255)                                             cTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTg                                                                                                       >  1:244330/1‑150 (MQ=255)                                              tAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTga                                                                                                      >  2:992178/1‑150 (MQ=255)                                               aaGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGATGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGTCAGCAGGTCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaa                                                                                                     <  2:1166097/150‑1 (MQ=255)                                                  gTACACCGCCTTCGCGTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcg                                                                                                  >  1:1678182/1‑150 (MQ=255)                                                    acacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaa                                                                                                <  1:2275024/150‑1 (MQ=255)                                                    acacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGCGTATTgaagcgaa                                                                                                >  2:1930457/1‑150 (MQ=255)                                                     cacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                               <  2:244330/150‑1 (MQ=255)                                                     aacCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaag                                                                                               <  2:587825/149‑1 (MQ=255)                                                      acCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTgaagcgaagc                                                                                              >  1:1428215/1‑150 (MQ=255)                                                        cGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGa                                                                                                                                 >  2:1677741/1‑113 (MQ=255)                                                        cGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGa                                                                                                                                 <  1:1677741/113‑1 (MQ=255)                                                            ggcGCCTGGCTCACGCGCCGTGCGCGCAAAAGCCCCTCTGGCATGTCGACCGCTACAGGTTGTTTGGCGGGGAAGTCCATCGCCAGCAGGCCATCAGCGCCACGGCTGACTCGCAACTCGCCACCGCGGGTATTGAAGCGAAGCACTTgc                                                                                        <  2:713240/148‑1 (MQ=255)                                                              cGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTgcgc                                                                                      <  2:1640473/150‑1 (MQ=255)                                                               gCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGcc                                                                                     >  1:103392/1‑150 (MQ=255)                                                                 cTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTg                                                                                   >  2:1829591/1‑150 (MQ=255)                                                                  tGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGc                                                                                  >  2:1555026/1‑150 (MQ=255)                                                                      tCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGGTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCAc                                                                              <  1:2180198/150‑1 (MQ=255)                                                                           cccAGCGCCTGCAAAAGGACCGTTGCGATGACACCCTCAACGCTGGGGGTGGGGTGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGt                                                                         <  2:1423808/150‑1 (MQ=255)                                                                            ccAGCGCCTGCAAAAGGCCCGCTGTCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGtt                                                                        <  2:106166/150‑1 (MQ=255)                                                                            ccAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCt                                                                                 >  1:2066059/1‑141 (MQ=255)                                                                            ccAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCt                                                                                 <  2:2066059/141‑1 (MQ=255)                                                                                cgcCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTc                                                                    <  1:1555026/150‑1 (MQ=255)                                                                                   cTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGaa                                                                 <  2:1332961/150‑1 (MQ=255)                                                                                   cTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTAGCTCGaa                                                                 <  1:1464306/150‑1 (MQ=255)                                                                                      cAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAg                                                              >  1:874116/1‑150 (MQ=255)                                                                                          aGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACg                                                          >  1:724173/1‑150 (MQ=255)                                                                                              ccGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAg                                                      <  1:1160752/150‑1 (MQ=255)                                                                                                  tGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCCCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGa                                                  <  1:2306852/150‑1 (MQ=255)                                                                                                    gCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTAtt                                                                                                        <  2:884350/94‑1 (MQ=255)                                                                                                    gCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTAtt                                                                                                        >  1:884350/1‑94 (MQ=255)                                                                                                      aTGTCGACCGCTACGGGTTGTTTGGCGGTGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTc                                                                    <  1:549476/128‑1 (MQ=255)                                                                                                      aTGTCGACCGCTACGGGTTGTTTGGCGGTGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTc                                                                    >  2:549476/1‑128 (MQ=255)                                                                                                        gTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCaa                                            >  1:1029454/1‑150 (MQ=255)                                                                                                                cTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCAt                                    <  1:1829591/150‑1 (MQ=255)                                                                                                                          tttGGCGGTGAATTTCATGGTTAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGACACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGTTCACCCAGTTGCTCGAACAGCACGTAAGCGGAGGCCAAGATAGCATGGCCGCACAg                          >  2:923951/1‑150 (MQ=255)                                                                                                                                     aGTCCATGGCAAGCAGGCCATCAGCGCCACGGCTGCGGCGCAACTCACCACAACGGGAATTGCAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCAAACAGCACGTGAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCa               >  2:1853751/1‑150 (MQ=255)                                                                                                                                      gctgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAg                                                 >  1:1052986/5‑115 (MQ=255)                                                                                                                                      gctgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAg                                                 <  2:1052986/111‑1 (MQ=255)                                                                                                                                      gTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCAc              >  2:2299207/1‑150 (MQ=255)                                                                                                                                       tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg             >  1:853545/1‑150 (MQ=255)                                                                                                                                       tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg             >  1:671040/1‑150 (MQ=255)                                                                                                                                       tCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACg             >  2:353989/1‑150 (MQ=255)                                                                                                                                       ctgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACtt                                                                                          <  2:903953/70‑1 (MQ=255)                                                                                                                                       ctgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACtt                                                                                          >  1:903953/4‑73 (MQ=255)                                                                                                                                        tgaTGGCCTGCTGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACgg            >  2:1510639/3‑150 (MQ=255)                                                                                                                                         cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAg                                                 <  2:1672677/112‑1 (MQ=255)                                                                                                                                         cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAg                                                 >  1:1672677/1‑112 (MQ=255)                                                                                                                                         cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGt           >  1:1758390/1‑150 (MQ=255)                                                                                                                                         cATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGt           <  1:184046/150‑1 (MQ=255)                                                                                                                                             gCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTgggg       <  2:874116/150‑1 (MQ=255)                                                                                                                                             gCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTgggg       <  1:2310030/150‑1 (MQ=255)                                                                                                                                             gCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTACGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTgggg       <  2:846003/150‑1 (MQ=255)                                                                                                                                             cccaccaGGCCATCAGCCCCACGGCTGACGCGCACCTCCCCACTGCCCGTATTGAACCGAAGCATTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTACTCCGAGGCCAAGGTAGCATGGCCCCACAGGTCGACTTCCACGGTgggg       <  1:923951/149‑1 (MQ=255)                                                                                                                                              ccAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTg          <  1:848639/146‑1 (MQ=255)                                                                                                                                              ccAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTg          >  2:848639/1‑146 (MQ=255)                                                                                                                                               cagcagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGt     >  2:939319/1‑150 (MQ=255)                                                                                                                                                agcagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAg                                       <  2:516983/115‑1 (MQ=255)                                                                                                                                                agcagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAg                                       >  1:516983/1‑115 (MQ=255)                                                                                                                                                  tagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGaa  <  2:1572134/149‑1 (MQ=255)                                                                                                                                                  cagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGTGTGaa  <  2:580949/150‑1 (MQ=255)                                                                                                                                                  cagGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGaa  <  2:103392/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     TTTCGAGCAGCATGGCGTCATCGATCACCACGACATAATCGTCGCTAAGGTACACCGCCTTCGCCTGGCTCAAGCCCAGCGCCTGCAAAAGGCCCGCTGGCATGTCGACCGCTACGGGTTGTTTGGCGGGGAAGTCCATGGCCAGCAGGCCATCAGCGCCACGGCTGACGCGCAACTCGCCACTGCGGGTATTGAAGCGAAGCACTTGCGCCTGCTCACCCAGTTGCTCGAACAGCACGTAAGCCGAGGCCAAGGTAGCATGGCCGCACAGGTCGACTTCCACGGTGGGGGTGAA  >  NC_002947/4906365‑4906659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑