Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	484,203	C→G	5.4%	G80A (GGC→GCC)	yeaG ←	protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	484,203	0	C	G	5.4%	294.8 / 7.0	92	G80A (GGC→GCC)	yeaG	protein kinase
Reads supporting (aligned to +/- strand):  ref base C (46/41);  new base G (3/2);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

TTTTCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGA  >  NC_002947/484056‑484349                                                                                                                                                    |                                                                                                                                                   ttttCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTccatgcca                                                                                                                                                  >  2:1956506/1‑150 (MQ=255)        tCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCAGCCGCTTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTTGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATAGAAGt                                                                                                                                           >  2:223382/1‑150 (MQ=255)          aGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGTGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGGCATGGAAgagt                                                                                                                                         >  1:1220991/1‑147 (MQ=255)             tgctTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGTGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGGCATGGAAgagtgca                                                                                                                                      <  2:1220991/150‑7 (MQ=255)               ctTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCaaa                                                                                                                                    >  1:277700/1‑150 (MQ=255)                ttGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGaa                                                                                                                                             <  2:1062384/140‑1 (MQ=255)                ttGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAg                                                                                                                                   >  1:1857427/1‑150 (MQ=255)                ttGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGACCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGag                                                                                                                                             >  1:1062384/1‑139 (MQ=255)                 tGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAgg                                                                                                                                  <  2:350237/150‑1 (MQ=255)                              aaGCGAGGACTCGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAgcggcg                                                                                                                     >  2:2205082/1‑150 (MQ=255)                                gCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGGCATGGAAgagt                                                                                                                                         <  1:2180606/128‑4 (MQ=255)                                gCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGGCATGGAAgagt                                                                                                                                         >  2:2180606/1‑125 (MQ=255)                                gCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGa                                                                                                                   >  1:2174084/1‑150 (MQ=255)                                gCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGa                                                                                                                   >  2:159561/1‑150 (MQ=255)                                 cGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGAt                                                                                                                  <  2:277700/150‑1 (MQ=255)                                   aGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCTGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATaa                                                                                                                >  2:1508564/1‑150 (MQ=255)                                   aGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATaa                                                                                                                >  1:13170/1‑150 (MQ=255)                                        ttGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACAACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCttg                                                                                                           >  1:1096711/1‑150 (MQ=255)                                             gccgccTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGga                                                                                                      <  2:1733852/150‑1 (MQ=255)                                                   tACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGAt                                                                                                >  1:1728822/1‑150 (MQ=255)                                                     ccGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCGTCCGGTCCTTGGGCGGCGTGGACGCAGTACCGCTTAAGGTGGGCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATgc                                                                                              <  1:1571782/150‑1 (MQ=255)                                                           ccAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACa                                                                                        >  2:116390/1‑150 (MQ=255)                                                             aggagATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGc                                                                                      >  1:2331692/4‑150 (MQ=255)                                                             agcagATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGc                                                                                      >  1:615926/1‑150 (MQ=255)                                                                agATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGa                                                                                                                   <  2:1664036/118‑1 (MQ=255)                                                                agATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGa                                                                                                                   >  1:1664036/1‑118 (MQ=255)                                                                agATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTg                                                                                   <  2:1857427/150‑1 (MQ=255)                                                                  aTAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGa                                                                                 >  2:445131/1‑150 (MQ=255)                                                                  aTAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGa                                                                                 <  2:247886/150‑1 (MQ=255)                                                                     gagGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGtt                                                                              >  1:1001362/1‑150 (MQ=255)                                                                       ggATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTgg                                                                            <  1:445131/150‑1 (MQ=255)                                                                       ggATCTGTTTCTTCTCTTCCAGGCCTTCGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTgg                                                                            <  2:882112/150‑1 (MQ=255)                                                                         aTCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGGAGTACGAGACGATCTGGGCGAGGCACTCTTCCATGCCACGGAAGTCGGCAAAGGCCGGATAGCGGCGGATGACATTGTTGTAAAAGATGCGAGACAGCCTGGAGATGGTa                                                                          >  2:900239/1‑150 (MQ=255)                                                                         aTCTGTTTCTCCGCTCCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTa                                                                          <  1:900239/150‑1 (MQ=255)                                                                           cTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGGAGATGCGCGACAGCCTGGAGTTgg                                                                            >  2:18798/1‑146 (MQ=255)                                                                           cTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGGAGATGCGCGACAGCCTGGAGTTgg                                                                            <  1:18798/146‑1 (MQ=255)                                                                             gTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGaa                                                                                                                                             >  2:2196363/1‑79 (MQ=255)                                                                             gTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGaa                                                                                                                                             <  1:2196363/79‑1 (MQ=255)                                                                             gTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTgg                                                                            >  2:32822/1‑144 (MQ=255)                                                                             gTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCAGGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTgg                                                                            <  1:32822/144‑1 (MQ=255)                                                                              tttCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTg                                                                             >  1:1537850/1‑142 (MQ=255)                                                                              tttCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTg                                                                             <  2:1537850/142‑1 (MQ=255)                                                                              tttCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgt                                                                     >  1:1302358/1‑150 (MQ=255)                                                                              tttCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgt                                                                     <  2:1302358/150‑1 (MQ=255)                                                                              tttCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgt                                                                     >  1:209953/1‑150 (MQ=255)                                                                                  ttctcttcCAGGCCTTGGGCGGCGTGGCGGAATTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCg                                                                 <  2:1555361/150‑1 (MQ=255)                                                                                   tctcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGGGACAGCCTGGAGTTGGTGGAGgcgttgg                                                                >  1:344897/1‑147 (MQ=255)                                                                                    ctcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGAt                                                               >  2:531273/1‑150 (MQ=255)                                                                                    ctcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGAt                                                               <  2:2331692/150‑1 (MQ=255)                                                                                     tcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATc                                                              <  2:2040356/150‑1 (MQ=255)                                                                                     tcttcCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATc                                                              <  1:1956506/150‑1 (MQ=255)                                                                                         ccgggCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGgaga                                                                                                    <  1:2325667/105‑1 (MQ=255)                                                                                         ccgggCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGgaga                                                                                                    >  2:2325667/4‑108 (MQ=255)                                                                                            ggCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTg                                                       <  2:2174084/150‑1 (MQ=255)                                                                                               cTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgtg                                                                    <  1:2333909/134‑1 (MQ=255)                                                                                               cTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGgtg                                                                    >  2:2333909/1‑134 (MQ=255)                                                                                                    gcggcgTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCg                                               >  1:1183950/1‑150 (MQ=255)                                                                                                      ggcgTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGAt                                             <  1:116390/150‑1 (MQ=255)                                                                                                       gcgTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATg                                            <  2:1183950/150‑1 (MQ=255)                                                                                                         gTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGc                                          <  2:1728822/150‑1 (MQ=255)                                                                                                         gTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGc                                          >  2:2076935/1‑150 (MQ=255)                                                                                                            gCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGt                                                                           <  2:605087/114‑1 (MQ=255)                                                                                                            gCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGt                                                                           >  1:605087/1‑114 (MQ=255)                                                                                                               gAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCaa                                    <  1:748530/150‑1 (MQ=255)                                                                                                                 aGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACa                                  <  1:1791456/150‑1 (MQ=255)                                                                                                                  gTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAg                                 <  1:2076935/150‑1 (MQ=255)                                                                                                                      gACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTa                                                                          >  1:1529738/1‑105 (MQ=255)                                                                                                                      gACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTa                                                                          <  2:1529738/105‑1 (MQ=255)                                                                                                                      gACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGt                             >  2:111303/1‑150 (MQ=255)                                                                                                                        ccatggttGGCCGATGCACTCTTACATACCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGCTCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTc                           <  2:1001362/143‑1 (MQ=255)                                                                                                                        cacGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTc                           <  2:209953/150‑1 (MQ=255)                                                                                                                           gATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACa                                                                                        <  2:1747835/86‑1 (MQ=255)                                                                                                                           gATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACa                                                                                        >  1:1747835/1‑86 (MQ=255)                                                                                                                             tCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGgccg                      >  1:126999/1‑150 (MQ=255)                                                                                                                                ggTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATg                                            >  2:2051733/1‑125 (MQ=255)                                                                                                                                ggTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATg                                            <  1:2051733/125‑1 (MQ=255)                                                                                                                                  tCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGAt                 >  2:2282285/1‑150 (MQ=255)                                                                                                                                    gATGCACTCTTCCATGCGATGGAAGTCGGCAAAGGCGGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATAAGCTCTGGCTCAACGATGGCAAGCAATAGAAGTTCGGCCGATGATGc               >  2:995708/1‑150 (MQ=255)                                                                                                                                    gATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGc               >  2:1235831/1‑150 (MQ=255)                                                                                                                                    gATGCACTCTTCCATGCCACGGAAGTCGACAAAGGCCGGATAGCGGCGGATAACCTTGTTCGAGAAGATGCCCTACAGCCTCGAGTTGGTATAGCTGTCCCTCAGCTCTGGCTCACCGTTGGCCAGCAACAGCCGTTCGGCCGCCGATGc               <  2:1025760/150‑1 (MQ=255)                                                                                                                                      tGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGt             >  1:1237491/1‑150 (MQ=255)                                                                                                                                      tGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGt             >  2:1045507/1‑150 (MQ=255)                                                                                                                                        cACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACCGCCGTTCGGCCGCCGATGCGTAg           <  2:1096711/150‑1 (MQ=255)                                                                                                                                         aCTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGc                                                     >  1:751623/1‑107 (MQ=255)                                                                                                                                         aCTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGc                                                     <  2:751623/107‑1 (MQ=255)                                                                                                                                         aCTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGt                             >  2:1218721/1‑131 (MQ=255)                                                                                                                                         aCTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGt                             <  1:1218721/131‑1 (MQ=255)                                                                                                                                         aCTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAgg          >  1:1409880/1‑150 (MQ=255)                                                                                                                                         aCTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCACCAACAGCCGTTCGGCCGCCGATGCGTAgg          >  2:294937/1‑150 (MQ=255)                                                                                                                                           actTCCATGGCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCATCAACAGCCGTTCGGCCGCCGATGCGTAGGCa        >  2:226411/2‑150 (MQ=255)                                                                                                                                             ttCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGc               <  2:291385/141‑1 (MQ=255)                                                                                                                                             ttCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGc               >  1:291385/1‑141 (MQ=255)                                                                                                                                              tCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTg     <  2:13170/150‑1 (MQ=255)                                                                                                                                                 atgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGa  >  2:1794471/1‑150 (MQ=255)                                                                                                                                                 atgccatgGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTAGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGa  >  2:2266200/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTTTCCATCAGCTGCTTGAGTTTTTCCGCAAGCGAGGACTTGCCGCCGCCTACCGGGCCCAGCAGATAGAGGATCTGTTTCTTCTCTTCCAGGCCTTGGGCGGCGTGGCGGAAGTACGACACGATCTGGTCGATGCACTCTTCCATGCCATGGAAGTCGGCAAAGGCCGGATAGCGGCGGATAACCTTGTTGGAGAAGATGCGCGACAGCCTGGAGTTGGTAGAGGTGTCGATCAGCTCTGGCTCACCGATGGCCAGCAACAGCCGTTCGGCCGCCGATGCGTAGGCACTGCGA  >  NC_002947/484056‑484349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑