Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,592,486	C→G	6.5%	S127R (AGC→AGG)	PP_4920 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,592,486	0	C	G	6.5%	291.8 / 10.5	93	S127R (AGC→AGG)	PP_4920	lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (44/43);  new base G (3/3);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

ACCGAAGACAATCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTCGAAGAATCGCACCGT  >  NC_002947/5592337‑5592633                                                                                                                                                      |                                                                                                                                                    aCCGAAGACAATCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCACCGAGTTGTCCAGc                                                                                                                                                     <  1:1584279/150‑1 (MQ=255) aCCGAAGACAATCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCAAGACGGACTGGTGATCATCGAGTTGTCCAGc                                                                                                                                                     >  2:1089784/1‑150 (MQ=255)     aaGACAATCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTAcc                                                                                                                                                 >  1:1313974/1‑150 (MQ=255)       gACAATCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTg                                                                                                                                               <  2:149367/150‑1 (MQ=255)          aaTCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGAc                                                                                                                                            >  1:211683/1‑150 (MQ=255)          aaTCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGAc                                                                                                                                            >  2:1985535/1‑150 (MQ=255)          aaTCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGAc                                                                                                                                            >  1:1375761/1‑150 (MQ=255)              aGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGGTAGCTGGACaact                                                                                                                                        <  1:232667/150‑4 (MQ=255)                   ggCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGgc                                                                                                                                   >  2:1005641/1‑150 (MQ=255)                    gcaccgcaccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGgcg                                                                                                                                  >  1:157159/1‑150 (MQ=255)                     caccgcaccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGCTGTCCAGGTAGCTGGACaactcga                                                                                                                                     >  2:324719/1‑140 (MQ=255)                     caccgcaccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGCTGTCCAGGTAGCTGGACaactcga                                                                                                                                     <  1:324719/146‑7 (MQ=255)                      accgcaccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGcc                                                                                                                                >  1:143624/1‑150 (MQ=255)                      accgcaccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGcc                                                                                                                                >  1:1124904/1‑150 (MQ=255)                        cgcaccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCa                                                                                                                              <  1:958659/150‑1 (MQ=255)                          caccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACg                                                                                                                            >  2:1410810/1‑150 (MQ=255)                           accAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACgg                                                                                                                           <  1:1410810/150‑1 (MQ=255)                            ccAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGc                                                                                                                          >  1:1707578/1‑150 (MQ=255)                             cAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCa                                                                                                                         >  1:605978/1‑150 (MQ=255)                             cAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCa                                                                                                                         >  1:572291/1‑150 (MQ=255)                              aGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCAt                                                                                                                        <  2:211683/150‑1 (MQ=255)                                 cACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATAATCGAATTGTCCAGCTACCTGATCAGCGTTGGCGCCCACGGCATGcc                                                                                                                     >  2:1776480/1‑150 (MQ=255)                                  aCCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGccc                                                                                                                    <  2:570188/150‑1 (MQ=255)                                    ccTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGAAAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCgg                                                                                                                  <  1:1776480/150‑1 (MQ=255)                                          ggCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCgg                                                                                                            <  2:1025330/150‑1 (MQ=255)                                            ccTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCt                                                                                                          <  1:431897/150‑1 (MQ=255)                                             cTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCGGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCtt                                                                                                         >  1:578572/1‑150 (MQ=255)                                              tACGAACAGCAATACCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTtc                                                                                                        <  2:430749/150‑1 (MQ=255)                                                  aaCAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTtcatca                                                                                                    >  1:1876981/1‑150 (MQ=255)                                                  aaCAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAACCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTtcatca                                                                                                    >  2:1560475/1‑150 (MQ=255)                                                   aCAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCaa                                                                                                   >  2:912729/1‑150 (MQ=255)                                                     aGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACt                                                                                                 >  1:182392/1‑150 (MQ=255)                                                     aGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACt                                                                                                 >  1:654710/1‑150 (MQ=255)                                                       cAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTAt                                                                                               >  1:1460077/1‑150 (MQ=255)                                                         atatCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGTCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTc                                                                                             <  2:286392/150‑1 (MQ=255)                                                          tatCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCa                                                                                            <  2:605978/150‑1 (MQ=255)                                                                 ccAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAAc                                                                                     <  1:1560475/150‑1 (MQ=255)                                                                  cAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTg                                                                                                                                     <  2:1412479/101‑1 (MQ=255)                                                                  cAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTg                                                                                                                                     >  1:1412479/1‑101 (MQ=255)                                                                    gTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTgg                                                                                  <  1:912729/150‑1 (MQ=255)                                                                     tGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGa                                                                                 >  1:1361136/1‑150 (MQ=255)                                                                     tGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGAGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGa                                                                                 <  2:654710/150‑1 (MQ=255)                                                                           cAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGt                                                                           >  2:1434153/1‑150 (MQ=255)                                                                            aaaCGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTg                                                                          >  2:1642495/1‑150 (MQ=255)                                                                            aaaCGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTg                                                                          >  1:628182/1‑150 (MQ=255)                                                                                gTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGa                                                                      <  1:1005641/150‑1 (MQ=255)                                                                                  aaCAGCGGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGAcc                                                                    <  2:578572/150‑1 (MQ=255)                                                                                   aCAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGAccc                                                                   <  2:1460077/150‑1 (MQ=255)                                                                                       cagcTACTTGCAAGCCAAGGTACGCGAGCAGGATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCa                                                               >  2:1437784/1‑150 (MQ=255)                                                                                       cagcTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTCACCCTGCa                                                               <  2:182392/150‑1 (MQ=255)                                                                                        agcTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAg                                                              <  1:1434153/150‑1 (MQ=255)                                                                                           tACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGAc                                                           <  1:1437784/150‑1 (MQ=255)                                                                                              ttGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCa                                                               >  1:876361/1‑143 (MQ=255)                                                                                              ttGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCa                                                               <  2:876361/143‑1 (MQ=255)                                                                                               tGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGAccc                                                                   >  1:2113480/1‑138 (MQ=255)                                                                                               tGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGAccc                                                                   <  2:2113480/138‑1 (MQ=255)                                                                                               tGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGc                                                       <  2:628182/150‑1 (MQ=255)                                                                                               tGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGc                                                       <  2:157159/150‑1 (MQ=255)                                                                                                gCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCt                                                      >  1:266587/1‑150 (MQ=255)                                                                                                  aaGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTgg                                                    >  1:279107/1‑150 (MQ=255)                                                                                                   aGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGt                                                   >  1:1438600/1‑150 (MQ=255)                                                                                                    gCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAg                                                                             <  1:787284/123‑1 (MQ=255)                                                                                                    gCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAg                                                                             >  2:787284/1‑123 (MQ=255)                                                                                                     ccAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGCCAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCACCTATTCACGCAAACTGGCCCAGGTGCTGACCCTGCAGGCCATGCGGgcgc                                                 >  2:1050657/1‑150 (MQ=255)                                                                                                      cAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCggg                                                                                                                 >  2:1370384/1‑85 (MQ=255)                                                                                                      cAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCggg                                                                                                                 <  1:1370384/85‑1 (MQ=255)                                                                                                      cAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGcc                                                >  2:472916/1‑150 (MQ=255)                                                                                                       aaGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGa                                                                      >  2:829085/1‑127 (MQ=255)                                                                                                       aaGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGa                                                                      <  1:829085/127‑1 (MQ=255)                                                                                                              gcgAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAgg                                        <  1:1620435/150‑1 (MQ=255)                                                                                                                  gcggcaGGACGGACTGGTGATCATCGAGTTGTCCAGCGACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACGGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGa                                    <  1:1050657/147‑1 (MQ=255)                                                                                                                  gcagcaTGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGa                                    <  2:1707578/150‑1 (MQ=255)                                                                                                                      caTGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTTCCCGGCCAGGAAGACAcc                                <  2:1375761/150‑1 (MQ=255)                                                                                                                          aCGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCa                                          >  2:494675/1‑136 (MQ=255)                                                                                                                          aCGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCa                                          <  1:494675/136‑1 (MQ=255)                                                                                                                           cGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTg                           <  2:1321446/150‑1 (MQ=255)                                                                                                                           cGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAAATATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTg                           <  1:1985535/150‑1 (MQ=255)                                                                                                                                  gTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTg                                                                          >  2:977500/1‑96 (MQ=255)                                                                                                                                  gTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTg                                                                          <  1:977500/96‑1 (MQ=255)                                                                                                                                    gATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGt                  <  1:1642495/150‑1 (MQ=255)                                                                                                                                     atcttcGAGTTGTCCATCTACCTGGATAGCGGGGGCGCCCACGTCATGACCGGGCGCGCGTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCTGGACTTGCGGGGCCCGGGGGAGGAAAACACCCTCTGGAAAACCGTc                 <  2:1098123/150‑1 (MQ=255)                                                                                                                                       cgtcGAGTTCTCCAGCTACCTGGACAGCTGTGGCTCCTGGGGCATGCCCGGGCGCGGCTTCATCGACTATTCACGCAAACTGGACGAGGTGCTGACCCTGCAGGACATGCTGTTGCCCGGCCAGGAAGACACCTCCTGGAAAACCTTCga               >  2:363312/3‑150 (MQ=255)                                                                                                                                        atcGAGTTGTCCAGCTACCTTGACAGTGGTGAACCTGGCTGCCTGCCCGGGCGCGCCTGCATCAACTACTCGCGCAAGCCGGACAAGGTGCTGACCCTCCAGGACAAGCTGGTGCCGGGCCAGGAAAACACCTTCTGGAGAGCCGTCgaa              <  2:777273/150‑1 (MQ=255)                                                                                                                                         tcGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGc                                            >  1:679648/1‑119 (MQ=255)                                                                                                                                         tcGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGc                                            <  2:679648/119‑1 (MQ=255)                                                                                                                                         tcGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTc                 <  1:297749/146‑1 (MQ=255)                                                                                                                                         tcGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTc                 >  2:297749/1‑146 (MQ=255)                                                                                                                                           ccgctGTCCAGGTAGCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTCgaagaa           >  1:1329760/5‑150 (MQ=255)                                                                                                                                            cgctGTCCAGGTAGCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGAcc                                                                    >  1:1463118/4‑92 (MQ=255)                                                                                                                                            cgctGTCCAGGTAGCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGAcc                                                                    <  2:1463118/89‑1 (MQ=255)                                                                                                                                             gTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTCGAAGAATc         <  2:1313974/150‑1 (MQ=255)                                                                                                                                              ttGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTCGAAGAATCg        <  2:572291/150‑1 (MQ=255)                                                                                                                                                gTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTTTGGAAAACCGTCGAAGAATCGCa      >  1:1944470/1‑150 (MQ=255)                                                                                                                                                   cAGCTACCTGGACAGCGGTGGCGCCCACGTCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTCGAAGAATCGCACCg   >  1:1592853/1‑150 (MQ=255)                                                                                                                                                    aGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGAGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTCGAAGAATCGCACCGt  >  1:1353955/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                    ACCGAAGACAATCAGCATGGCACCGCACCAGCCACCCTGCAGGCCTACGAACAGCAATATCTGGCCAGTGCCGACAAACGTAACAGCAGCTACTTGCAAGCCAAGGTACGCGAGCAGCATGACGGACTGGTGATCATCGAGTTGTCCAGCTACCTGGACAGCGGTGGCGCCCACGGCATGCCCGGGCGCGGCTTCATCAACTATTCACGCAAACTGGACAAGGTGCTGACCCTGCAGGACATGCTGGTGCCCGGCCAGGAAGACACCTTCTGGAAAACCGTCGAAGAATCGCACCGT  >  NC_002947/5592337‑5592633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑