Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,740,530 C→A 18.8% intergenic (+39/+32) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,5300CA18.8% 179.6 / 27.1 79intergenic (+39/+32)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (30/34);  new base A (8/7);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

GTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGT  >  NC_002947/5740381‑5740679
                                                                                                                                                     |                                                                                                                                                     
gTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGcc                                                                                                                                                       >  1:2063130/1‑150 (MQ=255)
       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCATCCCTTCGCGGGCTTGCACGCGAAg                                                                                                                                                <  1:470269/150‑1 (MQ=39)
       aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAg                                                                                                                                                <  2:1733316/150‑1 (MQ=255)
          tgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAgggc                                                                                                                                             <  1:814827/150‑2 (MQ=38)
           gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                            <  2:13225/150‑1 (MQ=255)
           gATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                            <  1:1442059/150‑1 (MQ=255)
            aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTg                                                                                                                                           >  1:1914649/1‑150 (MQ=255)
                  aaTGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaacgc                                                                                                                                     <  1:1487278/150‑1 (MQ=255)
                   aTGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaacaca                                                                                                                                    >  2:442311/1‑147 (MQ=255)
                     gggCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTgcaacgcaac                                                                                                                                  >  1:761766/1‑150 (MQ=255)
                                 gtgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTc                                                                                                                      >  1:2205737/1‑150 (MQ=255)
                                    cgcCAGGGGCTGCATGCCCAGGAAGATGAAAGCGGGAGGGTGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCTTCCCCAAAAAACTCAGt                                                                                                                   <  1:1191810/150‑1 (MQ=255)
                                    cgcCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGt                                                                                                                   <  2:194191/150‑1 (MQ=255)
                                      ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTgg                                                                                                                 >  1:377516/1‑149 (MQ=255)
                                      ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAg                                                                                                                                                >  1:853575/1‑119 (MQ=38)
                                      ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCGAAg                                                                                                                                                <  2:853575/119‑1 (MQ=37)
                                         ggggCGGCATGCCCAGGAATCTGATAGCCCGCGGGCGTTTGCCAAGGTGTTGCGCTAGCTTTTGGGCTGAGCGTATCGGGCTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCCACCCCAAAAAACTCAGTGCGtt                                                                                                              <  1:1504/150‑1 (MQ=255)
                                                    cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCACCGCAACCCCAAAAACCTCAGTGCATTTTTACTATTTg                                                                                                   <  1:1635499/150‑1 (MQ=255)
                                                    cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                                   <  1:1634213/150‑1 (MQ=255)
                                                    cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                                   <  2:2063130/150‑1 (MQ=255)
                                                    cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGCTTGCACGCGAAgggct                                                                                                                                            <  2:627956/109‑3 (MQ=37)
                                                    cccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGGGCTTGCACGCGAAgggct                                                                                                                                            >  1:627956/1‑107 (MQ=37)
                                                     ccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGt                                                                                                                   >  2:1269772/1‑133 (MQ=255)
                                                     ccAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGt                                                                                                                   <  1:1269772/133‑1 (MQ=255)
                                                         gAAGATGAAAGCGGGCGGGCGTTTGCCCAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGAt                                                                                              <  2:1443534/150‑1 (MQ=255)
                                                          aaGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGAGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                             <  1:693768/150‑1 (MQ=255)
                                                             aTGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGa                                                                                          <  2:1577176/150‑1 (MQ=255)
                                                                aaaGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgc                                                                                       >  1:687446/1‑150 (MQ=255)
                                                                     gggcgggcGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgcccgcc                                                                                  >  1:209434/1‑150 (MQ=255)
                                                                       gcgggcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGCGGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTGGCAACGCAAACCCAAAAAACCCAGTGCATTTTTACTATTTGCTGATCGCTCGCCCGCAAg                                                                                >  2:2142480/1‑150 (MQ=255)
                                                                         gggcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGAt                                                                              >  2:1219044/1‑150 (MQ=255)
                                                                             gattGCCTTGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGGTGCGTTGCAGCCCTTCGCGTGCAAGCCTGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTAATATTTGCTGATCGGAGGCCCGCCAGATAAAg                                                                          >  2:1161279/3‑150 (MQ=255)
                                                                                 gCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                                      <  2:2205737/150‑1 (MQ=255)
                                                                                 gCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGc                                                                      >  2:1028813/1‑150 (MQ=255)
                                                                                      ggTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgtg                                                                 >  2:1186709/1‑150 (MQ=255)
                                                                                          ttGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGNAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCAt                                                             >  1:1887418/1‑150 (MQ=255)
                                                                                              gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCACCCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGAt                                                                              <  2:2142238/129‑1 (MQ=255)
                                                                                              gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGAt                                                                              >  1:2142238/1‑129 (MQ=255)
                                                                                               ccAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcccc                                                        >  1:2062967/1‑150 (MQ=255)
                                                                                               ccAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcccc                                                        >  1:1667427/1‑150 (MQ=255)
                                                                                                cAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGaa                                                                                                                                                 <  1:373802/60‑1 (MQ=255)
                                                                                                cAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGaa                                                                                                                                                 >  2:373802/1‑60 (MQ=255)
                                                                                                   cTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgct                                                    >  2:2175736/1‑150 (MQ=255)
                                                                                                     tttGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCAGTGAGTGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTCTTACGTTCCGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCtgctgc                                                  <  1:1591609/150‑1 (MQ=255)
                                                                                                           cTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGcc                                            >  1:85444/1‑150 (MQ=255)
                                                                                                             gAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                                          <  2:2062967/150‑1 (MQ=255)
                                                                                                              aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                         <  2:1914649/150‑1 (MQ=255)
                                                                                                              aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                         <  1:1028813/150‑1 (MQ=255)
                                                                                                               gCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAg                                                                                                                    <  2:1052743/74‑1 (MQ=255)
                                                                                                               gCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAg                                                                                                                    >  1:1052743/1‑74 (MQ=255)
                                                                                                               gCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGa                                                                                          >  2:968565/1‑100 (MQ=255)
                                                                                                               gCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGa                                                                                          <  1:968565/100‑1 (MQ=255)
                                                                                                                  tACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                         >  1:2121386/1‑114 (MQ=255)
                                                                                                                  tACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGc                                                                         <  2:2121386/114‑1 (MQ=255)
                                                                                                                     cGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                                  <  2:209434/150‑1 (MQ=255)
                                                                                                                       gtgttgcgttgcAGACCTTCGCGTGCAAGTCCGCGAACGGTTGCAACGCCGCCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAgg                                <  1:2142480/148‑1 (MQ=255)
                                                                                                                        ggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                                  <  2:2243668/147‑1 (MQ=255)
                                                                                                                        ggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                                  >  1:2243668/1‑147 (MQ=255)
                                                                                                                             cgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                          <  2:1321995/150‑1 (MQ=255)
                                                                                                                              gttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                                           <  2:674721/116‑1 (MQ=25)
                                                                                                                              gttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                                           >  1:674721/1‑116 (MQ=25)
                                                                                                                               ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                             >  2:1633280/1‑81 (MQ=255)
                                                                                                                               ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                             <  1:1633280/81‑1 (MQ=255)
                                                                                                                               ttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgtgt                                                                <  1:573843/110‑1 (MQ=25)
                                                                                                                               ttgcAACCCTTCGAGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGgtgt                                                                >  2:573843/1‑110 (MQ=25)
                                                                                                                                  caacccTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcg                                                                                        <  2:232701/80‑1 (MQ=17)
                                                                                                                                  caacccTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcg                                                                                        >  1:232701/4‑83 (MQ=17)
                                                                                                                                  cAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACACAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc                     <  1:442311/150‑1 (MQ=255)
                                                                                                                                   aacccTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                    >  2:1570913/3‑150 (MQ=38)
                                                                                                                                   aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                    <  2:85444/150‑1 (MQ=255)
                                                                                                                                   aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAAATCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc                    <  1:1094222/150‑1 (MQ=255)
                                                                                                                                    acccTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTccc                   >  2:12824/2‑150 (MQ=38)
                                                                                                                                      ccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg                 >  1:1516633/1‑150 (MQ=255)
                                                                                                                                      ccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg                 >  1:77756/1‑150 (MQ=255)
                                                                                                                                       cTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAgg                >  1:403517/1‑150 (MQ=255)
                                                                                                                                        ttCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGc               >  1:1157271/1‑150 (MQ=255)
                                                                                                                                             gTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGATCCCAGGCCGGct          >  1:34464/1‑149 (MQ=255)
                                                                                                                                               gCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgt        <  2:2128647/150‑1 (MQ=255)
                                                                                                                                                cAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgtg       >  2:1690640/1‑150 (MQ=255)
                                                                                                                                                   gCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCg    <  1:2247546/150‑1 (MQ=255)
                                                                                                                                                     ccGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGTGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgt  <  1:2175736/150‑1 (MQ=255)
                                                                                                                                                     ccGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                                   <  1:313951/117‑1 (MQ=255)
                                                                                                                                                     ccGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagc                                   >  2:313951/1‑117 (MQ=255)
                                                                                                                                                     ccGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGgt  <  1:1690640/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGGT  >  NC_002947/5740381‑5740679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑