Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,276	G→C	intergenic (+513/+240)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,276	0	G	C	100.0%	70.2 / NA	59	intergenic (+513/+240)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (32/27);  total (32/27)

TATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCT  >  NC_002947/4741183‑4741388                                                                                                 |                                                                                                                  tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgc                                                                                    >  1:5850560/1‑125 (MQ=255)   ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcgg                                                                                  >  1:5850532/1‑125 (MQ=255)   ttGAACTGGGTATAGGACCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcgg                                                                                  <  1:5850327/125‑1 (MQ=255)       aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGcc                                                                              <  1:5850331/125‑1 (MQ=255)       aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGcc                                                                              >  1:5850468/1‑125 (MQ=255)       aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGcc                                                                              <  1:5850332/125‑1 (MQ=255)        cTGGGTATAGGTCCACCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                             <  1:5850335/125‑1 (MQ=255)         tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                             <  1:5850336/124‑1 (MQ=255)         tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                            >  1:5850793/1‑125 (MQ=255)         tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                            >  1:5850610/1‑125 (MQ=255)           ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTGGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                                          <  1:5850338/125‑1 (MQ=255)            gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                         <  1:5850340/125‑1 (MQ=255)            gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                         >  1:5850691/1‑125 (MQ=255)            gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                         >  1:5850731/1‑125 (MQ=255)             tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCTTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                         >  1:5850728/1‑124 (MQ=255)              ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                       <  1:5850344/125‑1 (MQ=255)              ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                        >  1:5850809/1‑124 (MQ=255)               taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGCACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                      <  1:5850346/125‑1 (MQ=255)                aGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                     <  1:5850347/125‑1 (MQ=255)                  gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGTCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                   >  1:5850756/1‑125 (MQ=255)                  gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                    >  1:5850639/1‑124 (MQ=255)                    ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 <  1:5850440/125‑1 (MQ=255)                    ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 <  1:5850352/125‑1 (MQ=255)                    ccAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 >  1:5850375/1‑125 (MQ=255)                     cAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCAGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcac                                                                >  1:5850749/1‑125 (MQ=255)                      aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                               <  1:5850438/125‑1 (MQ=255)                       gCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 <  1:5850435/122‑1 (MQ=255)                       gCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                              <  1:5850430/125‑1 (MQ=255)                       gCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                              >  1:5850494/1‑125 (MQ=255)                        ctctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 <  1:5850384/121‑1 (MQ=255)                        ctctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                             >  1:5850572/1‑125 (MQ=255)                         tctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                             >  1:5850699/1‑124 (MQ=255)                          ctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                                            <  1:5850368/124‑1 (MQ=255)                          ctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                                            <  1:5850436/124‑1 (MQ=255)                             gTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                                        <  1:5850411/125‑1 (MQ=255)                             gTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                                        <  1:5850451/125‑1 (MQ=255)                              tCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                       <  1:5850467/125‑1 (MQ=255)                               cACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                      <  1:5850372/125‑1 (MQ=255)                               cACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGGACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                       <  1:5850363/124‑1 (MQ=255)                                aCCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAAGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                          >  1:5850865/1‑120 (MQ=255)                                aCCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                          >  1:5850766/1‑120 (MQ=255)                                 ccTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                    >  1:5850613/1‑125 (MQ=255)                                 ccTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCC‑GCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                                   >  1:5850838/1‑125 (MQ=255)                                                            acttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTc                            >  1:5850719/7‑124 (MQ=255)                                                            acttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTc                            >  1:5850786/7‑124 (MQ=255)                                                            acttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCCAGTTAGGCCTTGTACTTGGACCCGGCCACCTc                            >  1:5850814/7‑124 (MQ=255)                                                             cttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCAGGCCACCTc                            >  1:5850945/6‑123 (MQ=255)                                                              ttgcaTTCGTGAATCCAGGCAC‑AGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACt                         <  1:5850390/121‑1 (MQ=255)                                                                gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGa                       >  1:5850418/3‑125 (MQ=255)                                                                  aTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAgg                     >  1:5850707/1‑125 (MQ=255)                                                                   ttCGTGAATCCAGGCAC‑AGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAggg                    >  1:5850834/1‑125 (MQ=255)                                                                   ttCGTGAA‑TCCAGCTACAGGCCCCGGACACGTCCTCCTTCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTAGGACCCGGCCACCTCACt                         >  1:5851161/1‑120 (MQ=255)                                                                                ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATc         <  1:5850443/119‑1 (MQ=255)                                                                                 gcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATccc       >  1:5850479/5‑125 (MQ=255)                                                                                   acaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATccc       <  1:5850426/121‑1 (MQ=255)                                                                                    caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc    >  1:5850876/2‑125 (MQ=255)                                                                                    caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc    >  1:5851097/2‑125 (MQ=255)                                                                                      ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct  >  1:5850782/1‑125 (MQ=255)                                                                                      ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct  <  1:5850421/125‑1 (MQ=255)                                                                                                 |                                                                                                                  TATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCT  >  NC_002947/4741183‑4741388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑