Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,289	C→G	intergenic (+526/+227)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,289	0	C	G	100.0%	71.1 / NA	59	intergenic (+526/+227)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (32/27);  total (32/27)

GTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCT  >  NC_002947/4741182‑4741388                                                                                                                |                                                                                                    gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCa                                                                                      >  1:3757181/1‑124 (MQ=255) gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAg                                                                                     <  1:3757006/125‑1 (MQ=255) gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAg                                                                                     >  2:3757226/1‑125 (MQ=255)  tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgc                                                                                    <  1:3757008/125‑1 (MQ=255)   aTTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcg                                                                                   <  1:3757009/125‑1 (MQ=255)         cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                             <  1:3757011/125‑1 (MQ=255)         cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                             >  1:3757171/1‑125 (MQ=255)          tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                            <  2:3757012/125‑1 (MQ=255)            ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                                          >  2:3757257/1‑125 (MQ=255)            ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                                          >  1:3757186/1‑125 (MQ=255)             gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                         >  2:3757158/1‑125 (MQ=255)                taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                      >  2:3757235/1‑125 (MQ=255)                taGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                      >  2:3757443/1‑125 (MQ=255)                taGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                      >  1:3757180/1‑125 (MQ=255)                taGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                      <  1:3757014/125‑1 (MQ=255)                  ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                    >  2:3757076/1‑125 (MQ=255)                  ggTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                    >  1:3757206/1‑125 (MQ=255)                  ggTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                    >  2:3757238/1‑125 (MQ=255)                   gTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                   <  1:3757015/125‑1 (MQ=255)                    tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                   <  2:3757016/124‑1 (MQ=255)                       aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                               <  2:3757049/125‑1 (MQ=255)                       aGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                               >  2:3757216/1‑125 (MQ=255)                        gCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                              <  1:3757037/125‑1 (MQ=255)                         ctctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                               <  2:3757063/123‑1 (MQ=255)                           ctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                           >  1:3757189/1‑125 (MQ=255)                           ctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                           <  2:3757035/125‑1 (MQ=255)                           ctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                           <  1:3757027/125‑1 (MQ=255)                            tGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                          <  1:3757025/125‑1 (MQ=255)                            tGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                          >  1:3757296/1‑125 (MQ=255)                             ggTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                         <  1:3757031/125‑1 (MQ=255)                             ggTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                         >  2:3757212/1‑125 (MQ=255)                             ggTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                         <  1:3757047/125‑1 (MQ=255)                              gTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                         >  1:3757266/1‑124 (MQ=255)                               tCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                       >  1:3757199/1‑125 (MQ=255)                               tCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                       <  1:3757017/125‑1 (MQ=255)                               tCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                       <  1:3757029/125‑1 (MQ=255)                               tCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                       >  1:3757164/1‑125 (MQ=255)                                cACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                      >  2:3757229/1‑125 (MQ=255)                                 aCCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                          <  1:3757061/120‑1 (MQ=255)                                  ccTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                     <  1:3757068/124‑1 (MQ=255)                                   cTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                                   >  1:3757439/1‑125 (MQ=255)                                    ttCGTGCTTGAG‑AAGCTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                  <  1:3757062/125‑1 (MQ=255)                                                              cttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                          <  2:3757028/120‑1 (MQ=255)                                                              cttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                          >  2:3757239/6‑125 (MQ=255)                                                               ttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACt                         >  1:3757259/5‑125 (MQ=255)                                                                  caTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAg                      >  2:3757154/2‑125 (MQ=255)                                                                   aTTCGTGAATCCAGGCAC‑AGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAgg                     <  1:3757053/125‑1 (MQ=255)                                                                   aTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAgg                     >  2:3757220/1‑125 (MQ=255)                                                                    ttCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAggg                    >  1:3757224/1‑125 (MQ=255)                                                                    ttCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAggg                    >  1:3757218/1‑125 (MQ=255)                                                                                 ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc        >  1:3757365/6‑125 (MQ=255)                                                                                 ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc        >  2:3757270/6‑125 (MQ=255)                                                                                 ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc        <  2:3757038/120‑1 (MQ=255)                                                                                  gcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATccc       <  1:3757032/121‑1 (MQ=255)                                                                                    acaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATccc       >  1:3757276/3‑123 (MQ=255)                                                                                    acaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcg      >  2:3757302/3‑124 (MQ=255)                                                                                     caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc    >  1:3757262/2‑125 (MQ=255)                                                                                      aGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc    <  1:3757048/124‑1 (MQ=255)                                                                                       ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct  <  1:3757054/125‑1 (MQ=255)                                                                                                                |                                                                                                    GTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCT  >  NC_002947/4741182‑4741388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑