Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,298	C→T	intergenic (+535/+218)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,298	0	C	T	100.0%	159.9 / NA	54	intergenic (+535/+218)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/17);  total (37/17)

TTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCT  >  NC_002947/4741185‑4741388                                                                                                                      |                                                                                           ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgc                                                                                    >  2:3518499/1‑123 (MQ=255)    aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggcg                                                                                >  1:3518414/1‑124 (MQ=255)    aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcg                                                                                   <  2:3518359/121‑1 (MQ=255)     aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTGTGCGACTTCCTTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                               >  2:3518590/1‑124 (MQ=255)     aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                               >  2:3518475/1‑124 (MQ=255)       tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTAGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                                                                 >  2:3518473/1‑120 (MQ=255)        gggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                             <  2:3518360/123‑1 (MQ=255)         ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCAGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                             <  2:3518361/122‑1 (MQ=255)         ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                            <  2:3518362/123‑1 (MQ=255)           tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                         <  1:3518364/124‑1 (MQ=255)            ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                         >  2:3518681/1‑123 (MQ=255)             taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                        <  2:3518365/123‑1 (MQ=255)             taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                       >  2:3518525/1‑124 (MQ=255)             taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                       >  2:3518497/1‑124 (MQ=255)               ggTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                     >  1:3518582/1‑124 (MQ=255)               ggTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                     >  1:3518804/1‑124 (MQ=255)                 tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                       <  2:3518367/120‑1 (MQ=255)                 tCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAg                                                                                     >  2:3518565/1‑106 (MQ=255)                   cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 >  1:3518544/1‑124 (MQ=255)                   cAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 >  2:3518649/1‑124 (MQ=255)                    aGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                 >  1:3518529/1‑123 (MQ=255)                     gCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGCTCACg                                                                     >  1:3518588/1‑118 (MQ=255)                      ctctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                              <  2:3518374/124‑1 (MQ=255)                      ctctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcaca                                                               <  2:3518390/123‑1 (MQ=255)                        ctGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                                            >  1:3518619/1‑124 (MQ=255)                         tGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                           >  2:3518566/1‑124 (MQ=255)                         tGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcg                                                            <  2:3518414/123‑1 (MQ=255)                          ggTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                           <  2:3518416/123‑1 (MQ=255)                            tCACCTTCGTGCTTGAG‑CA‑GCTTCTACGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgcg                                                          >  2:3518501/1‑122 (MQ=255)                              aCCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                         >  1:3518656/1‑121 (MQ=255)                              aCCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                      >  1:3518609/1‑124 (MQ=255)                               ccTTCGTGCTTGAG‑CA‑GCTTCTTCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                     >  2:3518615/1‑124 (MQ=255)                               ccTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                     >  2:3518600/1‑124 (MQ=255)                               ccTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                     >  2:3518587/1‑124 (MQ=255)                               ccTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                     >  1:3518571/1‑124 (MQ=255)                                 ttCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                    >  2:3518560/1‑123 (MQ=255)                                                          acttgcaTTCGTGAATCCAGGCAC‑AGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTc                            <  2:3518410/118‑1 (MQ=255)                                                          acttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                             >  2:3518591/7‑123 (MQ=255)                                                           cttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                           >  1:3518773/6‑124 (MQ=255)                                                            ttgcaTTCGTGAATCCAGGCAC‑AGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                          <  2:3518394/120‑1 (MQ=255)                                                            ttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                          <  2:3518413/120‑1 (MQ=255)                                                             tgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACt                         >  2:3518586/4‑124 (MQ=255)                                                               caTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACt                         <  2:3518385/121‑1 (MQ=255)                                                                aTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAg                      <  2:3518393/124‑1 (MQ=255)                                                                 ttCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACt                         >  2:3518550/1‑120 (MQ=255)                                                                               gcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                             <  1:3518387/99‑1 (MQ=255)                                                                               gcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                             >  2:3518387/5‑103 (MQ=255)                                                                                 acaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATccc       >  1:3518433/3‑123 (MQ=255)                                                                                  caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc        >  2:3518462/2‑121 (MQ=255)                                                                                    ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                           >  2:3518594/1‑100 (MQ=255)                                                                                    ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc    >  1:3518700/1‑123 (MQ=255)                                                                                    ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc    >  1:3518502/1‑123 (MQ=255)                                                                                    ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc    >  2:3518687/1‑123 (MQ=255)                                                                                     gCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct  >  1:3518734/1‑124 (MQ=255)                                                                                                                      |                                                                                           TTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCT  >  NC_002947/4741185‑4741388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑