BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,498	1 bp→CG	intergenic (+75/‑89)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,497	1	.	C	76.8%	168.5 / 118.3	142	intergenic (+74/‑90)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand): ref base . (1/32); new base C (61/48); total (62/80)
Fisher's exact test for biased strand distribution p-value = 9.66e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_002947	1,499,498	0	T	G	77.5%	118.3 / 97.7	142	intergenic (+75/‑89)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (61/49); minor base C (1/31); total (62/80)
Fisher's exact test for biased strand distribution p-value = 2.32e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 152 total aligned reads displayed.

TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA  >  NC_002947/1499383‑1499621

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   agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCAc                                                                                                                        <  1:1192104/121‑1 (MQ=21)

   agagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCAcg                                                                                                                       >  2:1192334/1‑122 (MQ=25)

      gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCAcg‑c‑ccg                                                                                                                 >  2:1192259/1‑121 (MQ=21)

           aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCca                                                                                                            >  1:1192291/1‑124 (MQ=18)

             cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCcac                                                                                                           >  2:1192294/1‑123 (MQ=18)

               aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGa                                                                                                        <  2:1192106/124‑1 (MQ=18)

               aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGa                                                                                                        >  1:1192275/1‑124 (MQ=18)

                aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGa                                                                                                        >  1:1192252/1‑123 (MQ=18)

                aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGAc                                                                                                       <  1:1192108/124‑1 (MQ=18)

                  ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCAc                                                                                                                        <  2:1192110/106‑1 (MQ=18)

                   cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAg                                                                                                         >  2:1192387/1‑119 (MQ=17)

                    ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGa                                                                                                        >  2:1192416/1‑119 (MQ=17)

                         accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCa                                                                                              <  1:1192112/124‑1 (MQ=18)

                           cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCAt                                                                                             <  1:1192113/123‑1 (MQ=18)

                            acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCAt                                                                                             >  1:1192326/1‑122 (MQ=17)

                              agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGGCTGATTGCCAtt                                                                                            >  2:1192327/1‑121 (MQ=17)

                              agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTaa                                                                                          <  1:1192116/123‑1 (MQ=18)

                                cagGACTGAGGCTGCCAACCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCg                                                                                       <  1:1192120/124‑1 (MQ=17)

                                cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCAt                                                                                             <  2:1192121/118‑1 (MQ=17)

                                cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTa                                                                                           <  2:1192118/120‑1 (MQ=17)

                                cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAg                                                                                         <  1:1192117/122‑1 (MQ=18)

                                 agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                      >  2:1192318/1‑124 (MQ=18)

                                 agGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCg                                                                                       <  1:1192122/123‑1 (MQ=18)

                                  ggACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                      >  1:1192388/1‑123 (MQ=18)

                                   gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                                    <  2:1192125/124‑1 (MQ=18)

                                     cTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGc                                                                                        >  1:1192292/1‑118 (MQ=17)

                                      tGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                                 <  1:1192127/124‑1 (MQ=18)

                                      tGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCATTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                                 <  2:1192128/124‑1 (MQ=18)

                                       gAGGCTGCCAAACGGTGGGAGCGGGCATGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTa                                                                                 <  1:1192130/123‑1 (MQ=18)

                                        aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc                                                                               >  2:1192289/1‑124 (MQ=18)

                                          gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                             <  1:1192135/124‑1 (MQ=18)

                                          gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                             <  2:1192133/124‑1 (MQ=18)

                                          gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGc                                                                             <  2:1192134/124‑1 (MQ=18)

                                            tGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                              >  2:1192394/1‑121 (MQ=17)

                                              ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                                           <  2:1192137/122‑1 (MQ=17)

                                                aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa                                                                         <  2:1192140/122‑1 (MQ=18)

                                                aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAg                                                                        <  1:1192138/123‑1 (MQ=18)

                                                 aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt                                                                       >  1:1192444/1‑123 (MQ=18)

                                                 aaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt                                                                       >  1:1192407/1‑123 (MQ=18)

                                                   cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCGCAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa                                                                     <  1:1192141/123‑1 (MQ=18)

                                                     gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTAC‑G‑CGGGCACG‑CTCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg                                                                  <  2:1192143/124‑1 (MQ=18)

                                                     gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg                                                                  >  2:1192255/1‑124 (MQ=18)

                                                     gTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg                                                                  <  1:1192144/124‑1 (MQ=18)

                                                        ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                                >  1:1192277/1‑123 (MQ=18)

                                                          aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGt                                                             <  1:1192146/124‑1 (MQ=18)

                                                          aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                                <  2:1192147/121‑1 (MQ=18)

                                                           gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc                                                               >  1:1192307/1‑121 (MQ=18)

                                                            cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt                                                            <  2:1192149/123‑1 (MQ=18)

                                                              ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACCGc                                                                 >  1:1192304/1‑116 (MQ=18)

                                                              ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc                                                                <  2:1192150/117‑1 (MQ=18)

                                                               gCGAGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                        <  2:1192152/124‑1 (MQ=21)

                                                                cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg                                                       <  1:1192153/124‑1 (MQ=21)

                                                                 gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                      >  2:1192248/1‑124 (MQ=21)

                                                                 gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                      >  1:1192348/1‑124 (MQ=21)

                                                                  tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                      <  2:1192154/123‑1 (MQ=21)

                                                                    cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CTCTCCCACAGACTGATTTCCATTAAGCGGGCCTATCGCTAGAGTGACGGCCCg                                                              <  1:1192157/113‑1 (MQ=18)

                                                                    cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGc                                                   >  2:1192377/1‑124 (MQ=21)

                                                                      cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCAt                                                                                             <  2:1192159/80‑1 (MQ=11)

                                                                      cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                                 <  1:1192158/124‑1 (MQ=21)

                                                                      cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                                 >  2:1192299/1‑124 (MQ=25)

                                                                      cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGCTTTTTGTTGCCt                                                 >  1:1192432/1‑124 (MQ=21)

                                                                      cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                                 <  2:1192160/124‑1 (MQ=25)

                                                                       gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                                 >  1:1192352/1‑123 (MQ=25)

                                                                       gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt                                                 >  1:1192354/1‑123 (MQ=25)

                                                                        cgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg                                                                  >  1:1192240/1‑105 (MQ=18)

                                                                          aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCt                                             >  1:1192320/1‑124 (MQ=25)

                                                                          aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                              <  2:1192163/123‑1 (MQ=25)

                                                                           aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa                                            >  2:1192427/1‑124 (MQ=25)

                                                                           aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                      <  2:1192164/114‑1 (MQ=21)

                                                                            aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt                                                     <  1:1192166/114‑1 (MQ=25)

                                                                            aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                           <  1:1192165/124‑1 (MQ=25)

                                                                             cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                           <  1:1192168/123‑1 (MQ=25)

                                                                              aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa                                           <  1:1192169/122‑1 (MQ=25)

                                                                                gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGaa                                       <  2:1192171/124‑1 (MQ=25)

                                                                                 cgccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTCTTTGTTGCCTCGCTAACGAAt                                      <  2:1192172/124‑1 (MQ=25)

                                                                                   ccgcGGTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACg                                         >  2:1192443/1‑119 (MQ=25)

                                                                                        gTGCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc                                <  2:1192174/123‑1 (MQ=25)

                                                                                          gCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTa                                            <  2:1192176/109‑1 (MQ=25)

                                                                                          gCATGGCACCGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc                             >  1:1192329/1‑124 (MQ=37)

                                                                                             tggcaccGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt                          <  2:1192180/118‑1 (MQ=37)

                                                                                              ggcaccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt                          >  1:1192404/6‑123 (MQ=37)

                                                                                               gcaccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt                               <  1:1192182/113‑1 (MQ=37)

                                                                                               gcaccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc                            >  1:1192345/5‑120 (MQ=37)

                                                                                               gcaccGGCTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                           <  2:1192184/117‑1 (MQ=37)

                                                                                                 accGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                      >  1:1192463/3‑124 (MQ=38)

                                                                                                  ccGGCTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                      >  1:1192335/2‑123 (MQ=38)

                                                                                                      cTTTGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                 <  1:1192186/124‑1 (MQ=38)

                                                                                                      cTTTGCCGGTGT‑T‑CGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                 <  2:1192185/124‑1 (MQ=38)

                                                                                                        ttGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                <  2:1192188/123‑1 (MQ=38)

                                                                                                         tGCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa                 >  1:1192531/1‑121 (MQ=39)

                                                                                                          gCCGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa              >  1:1192386/1‑123 (MQ=39)

                                                                                                           ccGGTGT‑TCG‑CGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                         <  2:1192189/111‑1 (MQ=38)

                                                                                                                 tgttcgcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTCACGAATCATGACTCCCTTAGAACGCTATCAAGCa          >  2:1192260/7‑122 (MQ=255)

                                                                                                                 tgttcgcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg         >  1:1192532/7‑123 (MQ=255)

                                                                                                                 tgttcgcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTCTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc           >  1:1192451/7‑121 (MQ=255)

                                                                                                                  gttcgcGGGCACGCC‑CGCTCCCACAGACTGATTGTCATTAAGCGGGCCTATTGCTACAGTGACGGCCCTTTTTTTGTTGCCTCGCTAACGAATCATTACTCCCTTAGAACGCTATCAAGCAGa        >  2:1192376/6‑123 (MQ=255)

                                                                                                                   ttcgcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg            <  2:1192194/114‑1 (MQ=255)

                                                                                                                   ttcgcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc      <  2:1192193/120‑1 (MQ=255)

                                                                                                                      gcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg    <  1:1192195/122‑1 (MQ=255)

                                                                                                                       cGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa  >  1:1192414/1‑124 (MQ=255)

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TTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGT‑T‑T‑CGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAA  >  NC_002947/1499383‑1499621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`