Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,272	A→G	intergenic (+509/+244)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,272	0	A	G	100.0%	48.3 / NA	60	intergenic (+509/+244)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/23);  total (37/23)

TTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTC  >  NC_002947/4741185‑4741386                                                                                           |                                                                                                                    ttGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcgg                                                                                >  1:4667579/1‑125 (MQ=255)  tGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTCAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                                                               >  1:4667515/1‑125 (MQ=255)    aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                             <  1:4667320/125‑1 (MQ=255)     aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGcc                                                                            >  1:4667718/1‑125 (MQ=255)      cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                           <  2:4667322/125‑1 (MQ=255)      cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                           <  1:4667324/125‑1 (MQ=255)       tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                          >  2:4667648/1‑125 (MQ=255)       tGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                          >  2:4667693/1‑125 (MQ=255)         ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGa                                                                        <  2:4667326/125‑1 (MQ=255)          gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                           >  2:4667584/1‑121 (MQ=255)           tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                      <  1:4667328/125‑1 (MQ=255)           tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                      <  2:4667327/125‑1 (MQ=255)            ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                     <  1:4667329/125‑1 (MQ=255)             taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                    >  2:4667368/1‑125 (MQ=255)             taGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                    >  2:4667678/1‑125 (MQ=255)               ggTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                   <  2:4667330/124‑1 (MQ=255)                gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACgg                                                                  >  2:4667864/1‑124 (MQ=255)                gTCAAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                   >  2:4667554/1‑123 (MQ=255)                 tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                 <  2:4667331/124‑1 (MQ=255)                 tCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                >  2:4667652/1‑125 (MQ=255)                  ccAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                               <  1:4667355/125‑1 (MQ=255)                   cAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                               >  1:4667472/1‑124 (MQ=255)                   cAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcac                                                              <  2:4667375/125‑1 (MQ=255)                    aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcac                                                              >  2:4667491/1‑124 (MQ=255)                    aGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACAAGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcac                                                              >  2:4667716/1‑124 (MQ=255)                    aGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTcac                                                              >  1:4667604/1‑124 (MQ=255)                     gCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                            >  2:4667814/1‑125 (MQ=255)                     gCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                            <  1:4667429/125‑1 (MQ=255)                     gCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                            >  2:4667653/1‑125 (MQ=255)                      ctctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                            <  2:4667414/124‑1 (MQ=255)                      ctctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                           >  1:4667592/1‑125 (MQ=255)                      ctctGGTCACCTCCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCacac                                                            <  1:4667381/124‑1 (MQ=255)                       tctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                           >  1:4667534/1‑124 (MQ=255)                        ctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                         <  1:4667428/125‑1 (MQ=255)                        ctGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                         >  2:4667612/1‑125 (MQ=255)                         tGGTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                         >  2:4667611/1‑124 (MQ=255)                          ggTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                       <  1:4667420/125‑1 (MQ=255)                           gTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                                      <  1:4667379/125‑1 (MQ=255)                           gTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                                      >  1:4667757/1‑125 (MQ=255)                           gTCACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGAt                                                      >  1:4667469/1‑125 (MQ=255)                           gTCACCTTCGTGCTTGAG‑CA‑GCTGCTGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                       <  2:4667380/124‑1 (MQ=255)                             cACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                    <  1:4667423/125‑1 (MQ=255)                             cACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                    <  1:4667394/125‑1 (MQ=255)                             cACCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAg                                                    >  2:4667713/1‑125 (MQ=255)                              aCCTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                   >  2:4667528/1‑125 (MQ=255)                               ccTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAATCCAGGCAC‑AGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                  <  1:4667389/125‑1 (MQ=255)                               ccTTCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                  >  1:4667545/1‑125 (MQ=255)                                 ttCGTGCTTGAGCAG‑CTTCTGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                >  1:4667573/1‑125 (MQ=255)                                                          acttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAcc                            >  2:4667885/7‑122 (MQ=255)                                                           cttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCAc                             >  2:4667591/6‑120 (MQ=255)                                                            ttgcaTTCGTGAATCCAGGCAC‑AGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                        >  1:4667802/5‑124 (MQ=255)                                                                aTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCa                         >  1:4667813/1‑119 (MQ=255)                                                                              ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc      >  2:4667599/6‑125 (MQ=255)                                                                              ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc      >  2:4667620/6‑125 (MQ=255)                                                                              ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc      >  1:4667665/6‑125 (MQ=255)                                                                              ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATc       <  2:4667372/119‑1 (MQ=255)                                                                                 acaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcg    <  1:4667424/122‑1 (MQ=255)                                                                                  caGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc  >  1:4667658/2‑125 (MQ=255)                                                                                   aGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcg    >  1:4667886/1‑122 (MQ=255)                                                                                    ggCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCAcgtc  >  2:4667909/1‑123 (MQ=255)                                                                                           |                                                                                                                    TTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCCGCCAACTGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTC  >  NC_002947/4741185‑4741386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑