Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,942,7600AT37.5% 10.4 / 14.3 16L429L (CTT→CTA) PP_2570transposase
Reads supporting (aligned to +/- strand):  ref base A (5/5);  new base T (2/4);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GATCGGGATATCACTCACACCTGAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGCTTC  >  NC_002947/2942612‑2942798
                                                                                                                                                    |                                      
gATCGGGATATCACTCACACCTGAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGaa                                       <  2:85908/150‑1 (MQ=255)
gATCGGAATATACCTAGCCCCTGAAGGTAGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGaa                                       <  2:719319/150‑1 (MQ=17)
         atCACTCACACCTGAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGAACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCTTCGCAAAGccc                              <  2:1100012/150‑1 (MQ=18)
                   ccTGAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTGTCCGGGCTTTGCTTCGCAAAGCCCGGaaactggg                    <  2:1144604/150‑8 (MQ=17)
                    cTGAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCTTCGCAAAGCCCg                             <  1:376328/140‑1 (MQ=255)
                    cTGAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCTTCGCAAAGCCCg                             >  2:376328/1‑140 (MQ=255)
                      gAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTc                 >  1:957031/1‑150 (MQ=255)
                        aGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCtt               <  1:754939/150‑1 (MQ=255)
                        aGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCtt               <  1:446585/150‑1 (MQ=255)
                        aGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCtt               <  2:957031/150‑1 (MQ=255)
                         ggTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCTTCGCAAAGCCCg                             <  1:661756/135‑1 (MQ=255)
                         ggTCGAGTTTGCCTGTGACGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCTTCGCAAAGCCCg                             >  2:661756/1‑135 (MQ=255)
                               gTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGcc                               <  1:821814/127‑1 (MQ=255)
                               gTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGcc                               >  2:821814/1‑127 (MQ=255)
                               gTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTtggcttct        >  2:396514/1‑150 (MQ=255)
                               gTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTtg              >  1:646147/1‑144 (MQ=255)
                               gTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTtg              <  2:646147/144‑1 (MQ=255)
                                     cggtgCCTGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGTGCTTTGCGAAGCAAAGCCCTGACCTGCCGGTCTtggcttctggcttt  >  1:185245/3‑149 (MQ=255)
                                                                                                                                                    |                                      
GATCGGGATATCACTCACACCTGAAGGTCGAGTTTGCCTGTGCCGGCCCCTTCGCGGGTGAACCCGCTCTCATCAAACAAAATGCAACCCATTGTTTTCTAAGCACTCAAGCGGCCACCAGTTGATACCCCAGTTTCCGGGCTTTGCGAAGCAAAGCCCGGACCTGCCGGTCTTGGCTTCTGGCTTC  >  NC_002947/2942612‑2942798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑