Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	527,931:1	+T	16.4%	noncoding (1166/2902 nt)	PP_23SC →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	527,931	1	.	T	16.4%	153.9 / 45.9	61	noncoding (1166/2902 nt)	PP_23SC	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base . (20/31);  new base T (3/7);  total (23/38)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01

TAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAG  >  NC_002947/527801‑528058                                                                                                                                     |                                                                                                                                 tAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑Cgatg                                                                                                                            <  1:299042/136‑1 (MQ=32)    aCAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGatgatg                                                                                                                         <  1:419982/136‑1 (MQ=32)          acgatgTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCAACGGGTATCA‑TCTTA‑‑CGATGATGCGGTag                                                                                                                   >  1:460286/6‑136 (MQ=12)           ggatgTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:642119/1‑136 (MQ=255)           ggatgTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:627790/1‑136 (MQ=255)           ggatgTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑Cgatg                                                                                                                            <  2:160880/125‑1 (MQ=11)           ggatgTTGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑Cgatg                                                                                                                            >  1:160880/1‑125 (MQ=11)           ggatgTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:163685/1‑136 (MQ=17)           ggatgTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:226471/1‑136 (MQ=17)           ggaggTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTaga                                                                                                                  >  1:562375/1‑136 (MQ=32)                 tGGCTTAGAAGCAGCCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCg                                                                                                            >  1:117511/1‑136 (MQ=12)                     ttAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCt                                                                                                        >  1:135122/1‑136 (MQ=32)                        taaGCAGCCACCCTTTAAAGAAAGCGTATTAGCTCACTAGTCGAGTCGGGCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTa                                                                                                     <  1:79475/135‑1 (MQ=12)                          agcagcCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACAGGTATCA‑TCTTA‑‑CGATGATGCTGTACAGGATCGTTCTGTAAg                                                                                                   >  2:270513/1‑136 (MQ=255)                             agcCATCATTTAAAGAAAGCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCt                                                                                                >  1:273469/1‑136 (MQ=12)                                 aCCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGa                                                                                            >  2:57000/1‑136 (MQ=32)                                 aCCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGa                                                                                            >  2:460001/1‑136 (MQ=32)                                            aaagCGTAATAGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTgtgctg                                                                                  >  1:303388/1‑131 (MQ=0)                                             aagCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAATAATGTGAAGGTGAGTTgag                                                                                <  1:135315/136‑1 (MQ=12)                                                 gTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGc                                                                            >  1:281630/1‑136 (MQ=32)                                                      aGCTCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       <  2:117511/136‑1 (MQ=17)                                                      aGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       <  2:579762/136‑1 (MQ=32)                                                      aGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCt                                                                       >  1:391247/1‑136 (MQ=32)                                                         tCACTGGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTgg                                                                     >  1:551397/1‑136 (MQ=0)                                                                     tcgGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGt                                                        >  1:460044/1‑136 (MQ=32)                                                                     tcgGCCTGCGCGGAAGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAg                                                         >  2:650262/1‑136 (MQ=0)                                                                          cTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGaa                                                   >  2:354611/1‑136 (MQ=32)                                                                               cgGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTg                                              <  1:140452/136‑1 (MQ=32)                                                                                 gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:661182/136‑1 (MQ=32)                                                                                 gAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:454076/136‑1 (MQ=32)                                                                                  aaGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGAc                                            <  2:30331/136‑1 (MQ=0)                                                                                   tgATGTAACGGGGCTCAAACCTTGCTCCGAAGGTACGGGTATGA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:627790/135‑1 (MQ=255)                                                                                   aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCT‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:358307/136‑1 (MQ=17)                                                                                   aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:511246/136‑1 (MQ=32)                                                                                   aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:546473/136‑1 (MQ=32)                                                                                   aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:163685/136‑1 (MQ=32)                                                                                   aGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:416454/136‑1 (MQ=32)                                                                                   aGATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACa                                           <  2:544602/136‑1 (MQ=0)                                                                                    gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGTATGCTGACATg                                         <  2:415240/136‑1 (MQ=17)                                                                                    gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                         <  2:226471/136‑1 (MQ=32)                                                                                    gATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATg                                         <  2:562375/136‑1 (MQ=32)                                                                                    gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTACTGTGAGGTATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:623273/136‑1 (MQ=0)                                                                                    gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGATGTATCAGAAGTGCGAATGCTGACAt                                          <  2:24214/136‑1 (MQ=0)                                                                                    gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:21523/136‑1 (MQ=0)                                                                                    gATGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAt                                          <  2:231097/136‑1 (MQ=0)                                                                                     aTGTAACGGGGCT‑AAACCATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTG‑GGTAGGGGAGCGTTCTGTAAGCCTGCGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACAta                                         <  2:233666/136‑2 (MQ=0)                                                                                               gCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACaa                              <  2:339759/136‑1 (MQ=32)                                                                                                 tCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATg                            >  1:262908/1‑136 (MQ=32)                                                                                                  cAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGc                           >  2:262715/1‑136 (MQ=32)                                                                                                            aCCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGaaaaa                 >  1:480716/1‑136 (MQ=32)                                                                                                                    tACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACg         <  2:135122/136‑1 (MQ=32)                                                                                                                         ttATCA‑TTTTA‑‑CGATTATGCGGTTGTGTAGGTTTCTTTAAGCCTGTTATGGTGTGTTTTGAAGCTTGCTGGTGTTATCAGAAGTGCGAATGCTGACATTTGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:460286/135‑1 (MQ=2)                                                                                                                         ttATCA‑TCTTA‑‑CTATTATGCGGTTGAGGAGGGTTCTGTATGCCTGTGAAGGTTTGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGGTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:133794/135‑1 (MQ=11)                                                                                                                         ttATCA‑TCTTA‑‑CGATGATGCGGTAGAGGTGCGTTCTGTAAGCCTGTGATGGTGTGTTGAGAAGCTTGCTGTAGGTATCTGATGTGCGAATGCTGACATGTGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:642119/135‑1 (MQ=11)                                                                                                                         gtttca‑tcTTA‑‑CGATGATGGGGTAGAGGAGGGTTGTGTAAGCCTGTGATGGTGTGTTGATAAGCTTGCTGGAGGTATCAGATGTGCTAATGCTGACATGAGTAACGACAATGCGTGTGAAAAACTCGCACGCCGaa    <  2:360482/133‑1 (MQ=11)                                                                                                                         gtttca‑taTTA‑‑CGATTTTGCGTTAGAGGAGCGTTCTGTAAGCCTGTGATGGTGAGTTGAGATGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:486053/133‑1 (MQ=11)                                                                                                                         gTATCT‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTTTGAAGGTGTGTTGAGATGCTTGCGGGAGGTATCAGAAGTGCGAATGCTGACATGATTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:455972/136‑1 (MQ=11)                                                                                                                         gTATCA‑TCTTA‑‑CGATGTGGCGGTAGAGGAGCGTTCTGTAAGCCTGTGATGGTGAGTTGAGAAGCTTGCTGGAGTTATCAGAAGTGCTAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:404425/136‑1 (MQ=11)                                                                                                                         gTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGaa    <  2:496150/136‑1 (MQ=32)                                                                                                                         gTATCA‑TATTA‑‑CGTTTATGCGGTAGAGGAGCGTTCTTTAAGCCTGTTTAGGTTTGTTGATAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGTGTGAAAACCTCGCACGCCGaa    <  2:130845/136‑1 (MQ=11)                                                                                                                           atca‑tcTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAg  <  2:435294/136‑1 (MQ=32)                                                                                                                                     |                                                                                                                                 TAGACAGCTAGGAGGTTGGCTTAGAAGCAGCCACCCTTTAAAGAAAGCGTAATAGCTCACTAGTCGAGTCGGCCTGCGCGGAAGATGTAACGGGGCTCAAACCATGCACCGAAGCTACGGGTATCA‑TCTTA‑‑CGATGATGCGGTAGAGGAGCGTTCTGTAAGCCTGTGAAGGTGAGTTGAGAAGCTTGCTGGAGGTATCAGAAGTGCGAATGCTGACATGAGTAACGACAATGCGAGTGAAAAACTCGCACGCCGAAAG  >  NC_002947/527801‑528058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑