Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,778,856	C→A	9.7%	A189A (GCC→GCA)	PP_5743 →	TonB‑dependent receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,778,856	0	C	A	9.7%	137.1 / 2.6	41	A189A (GCC→GCA)	PP_5743	TonB‑dependent receptor protein
Reads supporting (aligned to +/- strand):  ref base C (13/24);  new base A (2/2);  total (15/26)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

AGCCGCAGAAGTTCGGCAACACCTTCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGCAGCCTGCGCGGCGTAATGGCTTAGCAG  >  NC_002947/3778729‑3778989                                                                                                                                |                                                                                                                                      agccgcagAAGTTCGGCAACACCTTCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGAt                                                                                                                               >  1:123763/1‑136 (MQ=255) agccgcagAAGTTCGGCAACACCTTCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCAAGCCTGAt                                                                                                                               >  1:260567/1‑136 (MQ=255) agccgcagAAGTTCGGCAACACCTTCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCAAGCCTGAt                                                                                                                               >  1:581822/1‑136 (MQ=255)             tCGGCAACACCTTCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAgg                                                                                                                   >  1:404221/1‑136 (MQ=255)                gCAACACCTTCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGa                                                                                                                >  1:510058/1‑136 (MQ=255)                        ttCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACt                                                                                                        >  2:545807/1‑136 (MQ=255)                         tCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTa                                                                                                       <  2:64907/136‑1 (MQ=255)                         tCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTa                                                                                                       <  2:448074/136‑1 (MQ=255)                         tCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTa                                                                                                       <  2:147113/136‑1 (MQ=255)                           gcgGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTAcc                                                                                                     <  1:318211/136‑1 (MQ=255)                                     aCCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAg                                                                                           >  2:571241/1‑136 (MQ=255)                                       cTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCTACTACCACACACCCAGGc                                                                                         <  2:490842/136‑1 (MQ=255)                                       cTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGc                                                                                         <  1:471126/136‑1 (MQ=255)                                           cGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGTGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGt                                                                                     <  1:582148/136‑1 (MQ=255)                                           cGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGt                                                                                     <  1:608893/136‑1 (MQ=255)                                           cGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGt                                                                                     <  2:140740/136‑1 (MQ=255)                                            gCCCAAGGTTGCCGAGCATGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTg                                                                                    >  1:352088/1‑136 (MQ=255)                                                         gAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATggg                                                                       <  1:254941/136‑1 (MQ=255)                                                          aGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGTCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGc                                                                      >  2:605501/1‑136 (MQ=255)                                                          aGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGc                                                                      >  2:602643/1‑136 (MQ=255)                                                                  gACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCAt                                                              >  2:506625/1‑136 (MQ=255)                                                                    cAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATAc                                                            >  2:641468/1‑136 (MQ=255)                                                                     aaCCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACt                                                           <  1:331514/136‑1 (MQ=255)                                                                      accaccATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTg                                                          <  1:266639/136‑1 (MQ=255)                                                                       ccaccaTGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGc                                                         <  2:481175/136‑1 (MQ=255)                                                                            aTGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACgg                                                    >  2:629959/1‑136 (MQ=255)                                                                            aTGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACgg                                                    >  2:490854/1‑136 (MQ=255)                                                                             tGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGt                                                   <  1:510284/136‑1 (MQ=255)                                                                             tGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGt                                                   <  1:108665/136‑1 (MQ=255)                                                                             tGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGt                                                   <  1:329933/136‑1 (MQ=255)                                                                             tGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGt                                                   <  1:326642/136‑1 (MQ=255)                                                                             tGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGt                                                   <  2:123763/136‑1 (MQ=255)                                                                             tGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGt                                                   <  1:263625/136‑1 (MQ=255)                                                                                       cAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTa                                         >  1:401482/1‑136 (MQ=255)                                                                                         aCTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAAc                                       <  1:641468/136‑1 (MQ=255)                                                                                                   ggcAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGc                             <  2:352088/136‑1 (MQ=255)                                                                                                        cTTCCGCACGGCCACCGTACTAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATTCTGCCTCGGTTTCTGGTGTAACGGTCTGTCGCAGCCt                        <  1:92111/136‑1 (MQ=255)                                                                                                              cACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGCAGCCTGCgcgg                  <  2:603169/136‑1 (MQ=255)                                                                                                                         aaCAAGCAAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGCAGCCTGCGCGGCGTAATGGCtt       <  2:581822/136‑1 (MQ=255)                                                                                                                         aaCAAGCAAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGCAGCCTGCGCGGCGTAATGGCtt       <  2:260567/136‑1 (MQ=255)                                                                                                                          aCAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGCAGCCTGCGCGGCGTAATGGCTTa      <  1:605542/136‑1 (MQ=255)                                                                                                                              gccagccTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGCAGCCTGCGCGGCGTAATGGCTTAGCAg  <  1:377184/136‑1 (MQ=255)                                                                                                                                cagccTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTa                                         >  2:437837/1‑95 (MQ=255)                                                                                                                                cagccTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTa                                         <  1:437837/95‑1 (MQ=255)                                                                                                                                |                                                                                                                                      AGCCGCAGAAGTTCGGCAACACCTTCGCGGCCATCGACCTGACGCCCAAGGTTGCCGAGCAGGGCGACAACCACCATGGGGAGCTGCAACTCACCGGCGGCAGCTTCCGCACGGCCACCGAACAAGCCAGCCTGATTGGCCGCGAAGGCGATTTCGACTACCACACACCCAGGCACGTGCCCATGACATGGGCGTCTGCATACTGCCACGGTTTCTGGTGTAACGGTCTGTCGCAGCCTGCGCGGCGTAATGGCTTAGCAG  >  NC_002947/3778729‑3778989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑