Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,095,237	G→T	17.4%	G99C (GGC→TGC)	gudP →	glucarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,095,237	0	G	T	17.4%	60.7 / 3.7	23	G99C (GGC→TGC)	gudP	glucarate transporter
Reads supporting (aligned to +/- strand):  ref base G (9/10);  new base T (2/2);  total (11/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

GCCTTCGGATGGGCTTACGTGGCTGGCCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAACGCGCGCATCGTCGCCGCATGGTTCCCGACCCAGGA  >  NC_002947/4095105‑4095358                                                                                                                                     |                                                                                                                          gCCTTCGGATGGGCTTACGTGGCTGGCCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGcc                                                                                                                        >  2:666132/1‑136 (MQ=255) gCCTTCGGATGGGCTTACGTGGCTGGCCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGcc                                                                                                                        >  1:341406/1‑136 (MQ=255)   cTTCGGATGGGCTTACGTGGCTGGCCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTg                                                                                                                      <  2:2956/136‑1 (MQ=255)                     gtctggcCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGTGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTg                                                                                                    <  1:214038/134‑1 (MQ=255)                       ctggcCAGATTCCTGGTGTCTGGGTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCTGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTgtt                                                                                                  <  2:512827/136‑3 (MQ=255)                       ctggcCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGt                                                                                                  <  1:184068/136‑1 (MQ=255)                          gcCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCAc                                                                                               >  2:382767/1‑136 (MQ=255)                            cAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCAccc                                                                                             <  2:408703/136‑1 (MQ=255)                                            ggctACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCAGCGCtt                                                                             >  2:642035/1‑136 (MQ=255)                                             gctACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTTGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTc                                                                            >  2:385558/1‑136 (MQ=255)                                             gctACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTTGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTc                                                                            >  2:453157/1‑136 (MQ=255)                                                     aTCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCgg                                                                    <  2:346495/136‑1 (MQ=255)                                                     aTCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCgg                                                                    <  2:42652/136‑1 (MQ=255)                                                                   aaGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGccc                                                      <  2:159182/136‑1 (MQ=255)                                                                       aCGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTc                                                  >  1:479142/1‑136 (MQ=255)                                                                          tCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTTGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCAtt                                               <  1:453157/136‑1 (MQ=255)                                                                          tCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTTGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCAtt                                               <  1:385558/136‑1 (MQ=255)                                                                                      gCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAAcgc                                   >  2:387910/1‑136 (MQ=255)                                                                                             cACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAACGCGCGCAtc                            >  1:67717/1‑136 (MQ=255)                                                                                                   gTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAACGCGCGCATCGTcgcc                      <  1:666132/136‑1 (MQ=255)                                                                                                      gCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAACGCGCGCATCGTCGCCGCa                   >  1:266954/1‑136 (MQ=255)                                                                                                                  gctgcAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAACGCGCGCATCGTCGCCGCATGGTTCCCGAcc       <  2:530434/136‑1 (MQ=255)                                                                                                                       aGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAACGCGCGCATCGTCGCCGCATGGTTCCCGACCCAGGa  >  1:534206/1‑136 (MQ=255)                                                                                                                                     |                                                                                                                          GCCTTCGGATGGGCTTACGTGGCTGGCCAGATTCCTGGTGGCTGGCTACTCGATCGCTTCGGTTCCAAGAACGTCTACGCCTTCAGCATCTTCACCTGGTCGCTGTTCACCCTGCTGCAGGGTTTTGTCGGTGGCCTGCCGGTTGCCTGGGCCGTGGTCACCCTCTTCACCCTGCGCTTCCTGGTCGGTTTCGCCGAAGCCCCGTCATTCCCGGGTAACGCGCGCATCGTCGCCGCATGGTTCCCGACCCAGGA  >  NC_002947/4095105‑4095358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑