| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,328,603 | G→T | 28.5% | K66N (AAG→AAT) | PP_5631 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,328,603 | 0 | G | T | 28.5% | 26.2 / 4.2 | 14 | K66N (AAG→AAT) | PP_5631 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (5/5); new base T (2/2); total (7/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTGCAGGTACTTCTCTCGACCTACGCCAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCTTGCACAGCCAACGACATACCAACTTTTAGTGAGCCTCAACGTGAAAACATCCCTG > NC_002947/4328470‑4328716 | cGGTGCAGGTACTTCTCTCGACCTACGCCAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAATGc > 2:43953/1‑136 (MQ=255)cGGTGCAGGTACTTCTCTCGACCTACGCCAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAATGc > 2:43955/1‑136 (MQ=255) gCAGGTACTTCTCTCGACCTACGCCAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTc < 1:561773/136‑1 (MQ=255) ctcGACCTACGCCAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCa > 1:626595/1‑136 (MQ=255) ccGACCTACGCCAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGTGACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAg < 2:620500/135‑1 (MQ=255) ccAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTATGCGCCCTTTAGGCATAAGGAATTCGACAGCAATTCAGCAATACTCCa > 2:478534/1‑136 (MQ=255) cAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCaa > 2:263162/1‑136 (MQ=255) cGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCtt < 1:263162/136‑1 (MQ=255) cGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCtt < 2:596526/136‑1 (MQ=255) ggAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAATGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCTTGCACAGCCAACGACAt < 1:43953/136‑1 (MQ=255) ggAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAATGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCTTGCACAGCCAACGACAt < 1:43955/136‑1 (MQ=255) ggAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCTTGCACAGCCAACGACAt > 2:148663/1‑136 (MQ=255) atCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCTTGCACAGCCAACGACATACCAACTTTTAGTGAGCCTCAACGTGAAAAc < 1:273533/136‑1 (MQ=255) aCTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCTTGCACAGCCAACGACATACCAACTTTTAGTGAGCCTCAACGTGAAAACATCCCTg > 1:661570/1‑136 (MQ=255) | CGGTGCAGGTACTTCTCTCGACCTACGCCAAGTGGTTGAATTCTGCCAACGATTGGACGGAACTGGCGAAGCTGGAAAAGAACGTAATGGGTACAGGATTAAATATCGTTCACTTTGCGCCCTTTAGGCATAAGGCATTCGACAGCAATTCAGCCATACTCCAAAATGCATCGGTTTTAGGGGGAAAACCCTTGCACAGCCAACGACATACCAACTTTTAGTGAGCCTCAACGTGAAAACATCCCTG > NC_002947/4328470‑4328716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |