Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,649,261	T→A	19.1%	L378Q (CTG→CAG)	PP_4108 →	4‑aminobutyrate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,649,261	0	T	A	19.1%	38.0 / 5.9	21	L378Q (CTG→CAG)	PP_4108	4‑aminobutyrate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (6/11);  new base A (2/2);  total (8/13)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

TGAGCCCGTACATCGGCCGCCTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGATATCCTCGAGCAGTGCCTGGCAGAACTGA  >  NC_002947/4649129‑4649374                                                                                                                                     |                                                                                                                  tGAGCCCGTACATCGGCCGCCTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGAt                                                                                                                <  2:223401/136‑1 (MQ=255)    gCCCGTACATCGGCCGCCTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGcc                                                                                                             >  1:95008/1‑136 (MQ=255)     cccGTACATCGGCCGCCTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGccc                                                                                                            >  2:524833/1‑136 (MQ=255)                 ccgccTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGgcg                                                                                                >  2:428806/1‑136 (MQ=255)                   gccTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCTATTTTAATTCTGCTGATGCCCTGCGGCTAggattt                                                                                              <  1:524833/136‑5 (MQ=255)                   gccTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGcgcg                                                                                              <  2:61885/136‑1 (MQ=255)                            gCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCAcatcat                                                                                     <  1:601479/136‑1 (MQ=255)                               tGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCg                                                                                  <  2:138328/136‑1 (MQ=255)                                  gcgcCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCAGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCct                                                                               >  1:472174/1‑136 (MQ=255)                                  gcgcCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCAGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCct                                                                               >  1:475543/1‑136 (MQ=255)                                                      ttCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCg                                                           <  1:517987/136‑1 (MQ=255)                                                         gCCAAAGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGCgg                                                        <  1:142849/136‑1 (MQ=255)                                                           cAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCTAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCAGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGcc                                                      <  2:472174/136‑1 (MQ=255)                                                           cAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCGAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCAGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGcc                                                      <  2:475543/136‑1 (MQ=255)                                                                     ggCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCg                                            <  2:319579/136‑1 (MQ=255)                                                                                   ggcacAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGat                              <  2:123920/136‑1 (MQ=255)                                                                                     cacaACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGatat                            <  1:628794/136‑1 (MQ=255)                                                                                         aCTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGATATCCTc                        <  1:257852/136‑1 (MQ=255)                                                                                                          aGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGATATCCTCGAGCAGTGCCTGGCAga       >  1:666127/1‑136 (MQ=255)                                                                                                               gccCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGATATCCTCGAGCAGTGCCTGGCAgaactga  >  1:599615/1‑136 (MQ=255)                                                                                                               gccCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGATATCCTCGAGCAGTGCCTGGCAgaactga  >  1:265938/1‑136 (MQ=255)                                                                                                                                     |                                                                                                                  TGAGCCCGTACATCGGCCGCCTGACCGGCGTGGGCGCCATGCGAGGGATCGAGTTCGCCAATGCCGATGGCAGCCCGGCACCGGCACAACTGGCCAAGGTGATGGAGGCCGCCCGAGCCAGGGGCCTGCTGCTGATGCCCAGCGGCAAGGCGCGGCACATCATTCGCCTGCTGGCACCGCTGACTATCGAGGCCGAGGTGCTCGAGGAGGGGTTGGATATCCTCGAGCAGTGCCTGGCAGAACTGA  >  NC_002947/4649129‑4649374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑