| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 313,191 | C→T | 10.8% | C8Y (TGC→TAC) | fdhD ← | protein sulfur transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 313,191 | 0 | C | T | 10.8% | 106.1 / 3.7 | 37 | C8Y (TGC→TAC) | fdhD | protein sulfur transferase |
| Reads supporting (aligned to +/- strand): ref base C (16/17); new base T (2/2); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
TTGAGGCCGTTATAGACAATGGCCAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGTCTATGCCTTGATAGACCGCGTCGATTGGTCGCGCAG > NC_002947/313059‑313325 | ttGAGGCCGTTATAGACAATGGCCAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGa > 2:606743/1‑136 (MQ=255) gTTATAGACAATGGCCAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGc < 2:6591/136‑1 (MQ=255) taGACAATGGCCAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGTAGACCGGAGGCTTGc > 2:361755/1‑136 (MQ=255) ccAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCgg > 1:156202/1‑136 (MQ=255) ccAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCgg > 1:655031/1‑136 (MQ=255) cAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGTCAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCggg < 2:413813/136‑1 (MQ=255) cAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCggg < 2:310252/136‑1 (MQ=255) cACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTg > 1:443871/1‑136 (MQ=255) cACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCGGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCCTGGACAACGGCCATGAGGCCGCGCAGACCGGAGGCGTGCTGTTCATCGGGGCGCTg > 1:533450/1‑136 (MQ=255) ccTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATAc > 2:536520/1‑136 (MQ=255) ccTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATAc > 1:566779/1‑136 (MQ=255) cTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATAcc < 2:158204/136‑1 (MQ=255) cTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATAcc < 1:606743/136‑1 (MQ=255) cTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATAcc < 1:98969/136‑1 (MQ=255) ggCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACcggc < 2:655031/136‑1 (MQ=255) gCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGCCGTAGTCGTAGGGGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGGTTGGGGTTGATCCGGGCGCGGAAAACcggcg > 2:248786/1‑136 (MQ=255) ccAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACcggcgg > 1:464487/1‑136 (MQ=255) ccAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACcggcgg > 1:7578/1‑136 (MQ=255) cTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAg < 1:471244/136‑1 (MQ=255) cTGTCGCGCTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAg < 1:440400/136‑1 (MQ=255) gTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGTAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGca > 2:56316/1‑136 (MQ=255) ggCGTCGCTGAGCTGGACGTAGTAGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCCGAGGCTTGCTGTTAATAGGGGCGCTGAATACCGGCGGCTTTATTATATCACAAAccaaa > 2:229789/1‑133 (MQ=255) cGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGgc < 2:33186/136‑1 (MQ=255) tAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGt < 2:566779/136‑1 (MQ=255) tAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATc < 1:536520/136‑1 (MQ=255) aGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGTAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCg < 1:56316/136‑1 (MQ=255) gtgtTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCt > 1:646378/1‑136 (MQ=255) gtgtTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCt > 1:585721/1‑136 (MQ=255) gCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGt < 2:7578/136‑1 (MQ=255) gCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGt < 2:464487/136‑1 (MQ=255) gCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGt < 2:156202/136‑1 (MQ=255) tGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGgcggcg < 2:512945/119‑1 (MQ=255) tGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGgcggcg > 1:512945/1‑119 (MQ=255) gggCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGTCTATGCCtt > 2:355906/1‑136 (MQ=255) gggCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCAAGGGTGTATGCCtt > 2:225401/1‑136 (MQ=255) ggACAAGGGCGATGAGGCCGCGTAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGTCTATGCCTTGATAGa < 1:361755/136‑1 (MQ=255) ggCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGTCTATGCCTTGATAGACCGCGTCGATTGGTc < 2:646378/136‑1 (MQ=255) gcgcAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATAGCTAGGGTGTATTGCTTGATATACCGCGTCGATTGGTagcgg > 1:405211/1‑135 (MQ=255) gcAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGTCTATGCCTTGATAGACCGCGTCGATTGGTCGCGCAg > 2:374238/1‑136 (MQ=255) | TTGAGGCCGTTATAGACAATGGCCAGGGCCACTTCCTCGGCCAGCGGGGTGCTGTCGCGTTTGGCGTCGCTGAGCTGGACGTAGTCGTAGGTGTTGCTGGCGGCGGGCATGGACAAGGGCGATGAGGCCGCGCAGACCGGAGGCTTGCTGTTCATCGGGGCGCTGAATACCGGCGGCTTTCTGATAGCACAAGCCTAGGCGCGTGGGCCGGCGGCGTCTAATCGCTAGGGTCTATGCCTTGATAGACCGCGTCGATTGGTCGCGCAG > NC_002947/313059‑313325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |