Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,785,727 C→A 8.1% R2194L (CGG→CTG)  pvdJ ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,785,7270CA 8.1% 168.5 / 2.3 49R2194L (CGG→CTG) pvdJnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base C (14/31);  new base A (2/2);  total (16/33)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

AGCGCCTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGG  >  NC_002947/4785595‑4785859
                                                                                                                                    |                                                                                                                                    
agcGCCTGCGGATCCTGCGCGACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCa                                                                                                                                   <  2:534113/136‑1 (MQ=255)
agcGCCTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCa                                                                                                                                   <  2:38657/136‑1 (MQ=255)
  cgcCTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAgg                                                                                                                                 <  1:55495/136‑1 (MQ=255)
  cgcCTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAgg                                                                                                                                 >  2:119715/1‑136 (MQ=255)
  cgcCTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAgg                                                                                                                                 <  1:245810/136‑1 (MQ=255)
    ccTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGcc                                                                                                                               <  1:5981/136‑1 (MQ=255)
    ccTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGcc                                                                                                                               >  2:356985/1‑136 (MQ=255)
     cTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGccc                                                                                                                              >  1:105477/1‑136 (MQ=255)
      tGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCa                                                                                                                             >  2:306299/1‑136 (MQ=255)
      tGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCa                                                                                                                             >  1:515640/1‑136 (MQ=255)
      tGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCa                                                                                                                             >  1:228559/1‑136 (MQ=255)
       gCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCaa                                                                                                                            <  1:131991/136‑1 (MQ=255)
          gATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCAGCAGGCCCAAGTa                                                                                                                         >  1:558334/1‑136 (MQ=255)
          gATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCAGCAGGCCCAAGTa                                                                                                                         >  1:130417/1‑136 (MQ=255)
              cTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGt                                                                                                                     <  1:384101/136‑1 (MQ=255)
              cTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGt                                                                                                                     <  2:620775/136‑1 (MQ=255)
                gcgcCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAg                                                                                                                   >  1:370604/1‑136 (MQ=255)
                  gcCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGt                                                                                                                          <  2:585212/127‑1 (MQ=255)
                  gcCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGt                                                                                                                          >  1:585212/1‑127 (MQ=255)
                       ggCGTCGTGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTc                                                                                                            <  1:558931/136‑1 (MQ=255)
                       ggCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTc                                                                                                            <  1:404148/136‑1 (MQ=255)
                        gCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCAGCAGGCCCAAGTACGGTAGCTTGCTCa                                                                                                           <  2:558334/136‑1 (MQ=255)
                        gCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCAGCAGGCCCAAGTACGGTAGCTTGCTCa                                                                                                           <  2:130417/136‑1 (MQ=255)
                              gggAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGgt                                                                                                     >  1:538898/1‑136 (MQ=255)
                               ggAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGgtt                                                                                                    <  1:599161/136‑1 (MQ=255)
                                 aaGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGgttgt                                                                                                  <  2:378858/136‑1 (MQ=255)
                                 aaGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGgttgt                                                                                                  <  2:176522/136‑1 (MQ=255)
                                 aaGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACAGTAGCTTGCTCAGCTGgttgt                                                                                                  <  2:55467/136‑1 (MQ=255)
                                      ttCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGc                                                                                             >  2:340079/1‑136 (MQ=255)
                                           cgcgGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGcc                                                                                        <  1:356985/136‑1 (MQ=255)
                                           cgcgGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGcc                                                                                        <  1:37617/136‑1 (MQ=255)
                                                cAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCTGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCt                                                                                   <  1:167798/136‑1 (MQ=255)
                                                     acacaGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCg                                                                              >  1:540538/1‑136 (MQ=255)
                                                     acacaGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCg                                                                              >  1:357084/1‑136 (MQ=255)
                                                         aGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGGCTGTTCGACCa                                                                          <  1:342360/136‑1 (MQ=255)
                                                         aGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCa                                                                          <  2:171088/136‑1 (MQ=255)
                                                         aGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCa                                                                          <  1:369016/136‑1 (MQ=255)
                                                         aGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCa                                                                          <  1:41269/136‑1 (MQ=255)
                                                         aGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCa                                                                          <  1:343348/136‑1 (MQ=255)
                                                         aGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCa                                                                          <  2:475659/136‑1 (MQ=255)
                                                             ccgccgTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGAc                                                                      <  1:531253/136‑1 (MQ=255)
                                                                   tCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCttt                                                                >  2:163793/1‑136 (MQ=255)
                                                                                      gATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCa                                             <  1:555467/136‑1 (MQ=255)
                                                                                          tCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAg                                         >  1:398648/1‑136 (MQ=255)
                                                                                            aaaGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTg                                       <  2:673261/136‑1 (MQ=255)
                                                                                             aaGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTGa                                      <  2:515640/136‑1 (MQ=255)
                                                                                             aaGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTGa                                      <  2:228559/136‑1 (MQ=255)
                                                                                             aaGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTGa                                      <  1:163793/136‑1 (MQ=255)
                                                                                                                           tCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCAt        <  2:110895/136‑1 (MQ=255)
                                                                                                                                 ccccGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATgg  <  2:538898/136‑1 (MQ=255)
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AGCGCCTGCGGATCCTGCGCCACGGCGTCGGGGAAGATTTCCACGCGGCAACCACACAGGGCCGCCGTCAGCAATTGCCAGACGCTGATATCAAAGCTCTGCGAGGCCGTCTGGGCGATCACGTCGCTGCCCCGCAGGCCCAAGTACGGTAGCTTGCTCAGCTGGTTGTTGAGCATGCCGGCCTGTTCGACCATGACGCCTTTAGGCACACCGGTCGAACCAGAAGTGAAGATCACGTAGGCCAGCAGCTTGGCGGCATTGATGG  >  NC_002947/4785595‑4785859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑