| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 341,380 | C→T | 14.8% | intergenic (‑271/‑107) | PP_5438 ← / → gabP‑I | hypothetical protein/gamma‑aminobutyrate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 341,380 | 0 | C | T | 14.8% | 66.4 / 4.5 | 27 | intergenic (‑271/‑107) | PP_5438/gabP‑I | hypothetical protein/gamma‑aminobutyrate permease |
| Reads supporting (aligned to +/- strand): ref base C (9/14); new base T (2/2); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
TTTGTAAGTGGGTATTTCAGTAATCCGCTGATTTGAGGTCCTTTCGGTCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTAACAATTCCAATGACCTCGC > NC_002947/341252‑341515 | tttGTAAGTGGGTATTTCAGTAATCCGCTGATTTGAGGTCCTTTCGGGCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTAtt > 2:316435/1‑136 (MQ=255) gTAAGTGGGTATTTCAGTAATCCGCTGATTTGAGGTCCTTTCGGTCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGcc > 1:150303/1‑136 (MQ=255) gTAAGTGGGTATTTCAGTAATCCGCTGATTTGAGGTCCTTTCGGTCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGcc > 2:486990/1‑136 (MQ=255) tCCGCTGATTTGAGGTCCTTTCGGTCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGc < 2:443838/136‑1 (MQ=255) tCCGCTGATTTGAGGTCCTTTCGGTCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGACGCGCTTATTGCCAGTTGGAAACCGCCGATTGc < 2:364431/136‑1 (MQ=255) cccGCTGTTTTGTGGCCCTTGCGGTCTTGCAAGCGCCTAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGc < 2:644625/135‑1 (MQ=255) ttttGTGGTCCTTTCGGAATTGCATGGGCCTAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACg < 1:68497/135‑1 (MQ=255) tCCTTTCGGTCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTc > 1:584724/1‑136 (MQ=255) ttttCGGCCTTCCAATGGCCTAACGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCAt < 2:646402/135‑1 (MQ=255) ccAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGgcgcg < 2:342113/135‑1 (MQ=255) aaTGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGct > 2:417056/1‑136 (MQ=255) gAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTAcc > 1:345557/1‑136 (MQ=255) cTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTc < 2:584492/136‑1 (MQ=255) cTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTc < 2:150303/136‑1 (MQ=255) tCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTTATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCg < 2:74333/136‑1 (MQ=255) cAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGTGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGc > 2:501651/1‑136 (MQ=255) cAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGTGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGc > 2:504806/1‑136 (MQ=255) cGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGTGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGt < 1:504806/136‑1 (MQ=255) cGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGTGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGt < 1:501651/136‑1 (MQ=255) aTCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTTGAAACCGCCGATTGCAGCGACGCCTAGGTCAt > 1:577055/1‑98 (MQ=255) aTCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTTGAAACCGCCGATTGCAGCGACGCCTAGGTCAt < 2:577055/98‑1 (MQ=255) gCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACaa < 1:417056/136‑1 (MQ=255) gAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGG‑ACACAACTTACAAAAGTAAtt > 2:455308/1‑136 (MQ=255) cGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCg > 2:65492/1‑136 (MQ=255) tGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTaa < 2:620537/136‑1 (MQ=255) atatGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTAACAATTCCAAt < 2:495234/136‑1 (MQ=255) tatGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTAACAATTCCAATg < 1:316435/136‑1 (MQ=255) cgcTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTAACAATTCCAATGACCTCGc < 2:145047/136‑1 (MQ=255) cgcTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTAACAATTCCAATGACCTCGc < 2:584724/136‑1 (MQ=255) cgcTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTAACAATTCCAATGACCTCGc < 1:390489/136‑1 (MQ=255) | TTTGTAAGTGGGTATTTCAGTAATCCGCTGATTTGAGGTCCTTTCGGTCATGCAATGGCCGAAAGCCCTCCAGGCCGCATCCTAAAAGGCCTGCAGAATTTTCTGACGAGTGCATTCGTCATATGGCGCGCTTATTGCCAGTTGGAAACCGCCGATTGCAGCGACGCCTAGGTCATCCCCTGGCGCGCTCTGTACCCGCAGTCGGCGTGGTCCACTCCTTACAAAGGTAGTTTCAATGAGCGGTAACAATTCCAATGACCTCGC > NC_002947/341252‑341515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |