| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,067,659 | C→A | 26.6% | A15E (GCG→GAG) | PP_4460 → | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,067,659 | 0 | C | A | 26.6% | 28.9 / 4.3 | 15 | A15E (GCG→GAG) | PP_4460 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (5/6); new base A (2/2); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
AAGCTTTTGATTAGTAGAAGGAGCTCCCGTAAGCTAAAAAGATCATGTCTTTATGCTATCGGCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACAAGAACTAAGGGGCGCCTGGT > NC_002947/5067541‑5067794 | aaGCTTTTGATTAGTAGAAGGAGCTCCCGTAAGCTAAAAAGATCATGTCTTTATGCTATCGGCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAAt > 1:62936/1‑136 (MQ=255) tagtagAAGGAGCTCCCGTAAGCTAAAAAGATCATGTCTTTATGCTATCGGCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCggg > 2:640165/1‑135 (MQ=255) gAAGGAGCTCCCGTAAGCTAAAAAGATCATGTCTTTATGCTATCGGCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGtgctgc < 1:65746/136‑1 (MQ=255) gAAGGAGCTCCCGTAAGCTAAAAAGATCATGTCTTTATGCTATCGGCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGtgctgc < 2:373675/136‑1 (MQ=255) tCTTTATGCTATCGGCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTc < 1:32539/136‑1 (MQ=255) gCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGAGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGc > 1:16853/1‑136 (MQ=255) gCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGAGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGc > 1:61545/1‑136 (MQ=255) agaACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGAGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAAt > 1:475460/1‑136 (MQ=255) aaTCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTg < 1:504677/136‑1 (MQ=255) ttCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGtt < 1:258954/136‑1 (MQ=255) tCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACa < 1:665641/136‑1 (MQ=255) gAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTATTTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACAAGAACTa > 2:426377/1‑136 (MQ=255) gCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACAAGAACTAAgg > 2:408780/1‑136 (MQ=255) tttCGGGAGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACAAGAACTAAGGGgc < 2:16853/136‑1 (MQ=255) tttCGGGAGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACAAGAACTAAGGGgc < 2:61545/136‑1 (MQ=255) cGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACAAGAACTAAGGGGCGCCTGGt < 1:379462/136‑1 (MQ=255) | AAGCTTTTGATTAGTAGAAGGAGCTCCCGTAAGCTAAAAAGATCATGTCTTTATGCTATCGGCAGGTGGAGTACCATGGAGAACAGAATCACCCTTCGTCACCTAGAAGCGTTTCGGGCGATCATGGTGCGTAAATCCGTCACCGGTGCTGCAGAAATGCTTGAAGTGACCCAACCGGTAGTCACGAGGTTAATTGCCGATCTTGAGGAACGAATAGCACTGACACTGTTTACAAGAACTAAGGGGCGCCTGGT > NC_002947/5067541‑5067794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |